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Q97F65 (KITH_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:tdk
Ordered Locus Names:CA_C2887
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length195 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00124.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 195195Thymidine kinase HAMAP-Rule MF_00124
PRO_0000174967

Regions

Nucleotide binding15 – 228ATP HAMAP-Rule MF_00124
Nucleotide binding57 – 582ATP HAMAP-Rule MF_00124
Nucleotide binding88 – 914ATP By similarity

Sites

Active site891Proton acceptor Potential
Metal binding1451Zinc
Metal binding1481Zinc
Metal binding1831Zinc
Metal binding1861Zinc
Binding site231ATP
Binding site1201Substrate; via amide nitrogen By similarity
Binding site1791Substrate By similarity

Secondary structure

.................................... 195
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q97F65 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 6EDB551986B203BA

FASTA19522,303
        10         20         30         40         50         60 
MYRPKDHGWV EVIVGPMYSG KSEELIRRIR RAKIAKQKIQ VFKPEIDNRY SKEDVVSHMG 

        70         80         90        100        110        120 
EKEQAVAIKN SREILKYFEE DTEVIAIDEV QFFDDEIVEI VNKIAESGRR VICAGLDMDF 

       130        140        150        160        170        180 
RGKPFGPIPE LMAIAEFVDK IQAICVVCGN PATRTQRLIN GKPAFYDDPV VLIGAMESYE 

       190 
ARCRKCHVVP QKKEV 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]"X-ray structure of clostridium acetobutylicum genomics target CAR26."
Northeast structural genomics consortium (NESG)
Submitted (FEB-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-191 IN COMPLEX WITH ZINC IONS AND ADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK80830.1.
PIRC97255.
RefSeqNP_349490.1. NC_003030.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XX6X-ray2.00A/B1-191[»]
ProteinModelPortalQ97F65.
SMRQ97F65. Positions 2-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C2887.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK80830; AAK80830; CA_C2887.
GeneID1119070.
KEGGcac:CA_C2887.
PATRIC32040185. VBICloAce74127_3071.

Phylogenomic databases

eggNOGCOG1435.
HOGENOMHOG000076390.
KOK00857.
OMAKVHAICV.
OrthoDBEOG69D3J2.
ProtClustDBPRK04296.

Enzyme and pathway databases

BioCycCACE272562:GJIH-2970-MONOMER.
BRENDA2.7.1.21. 1452.

Family and domain databases

HAMAPMF_00124. Thymidine_kinase.
InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ97F65.

Entry information

Entry nameKITH_CLOAB
AccessionPrimary (citable) accession number: Q97F65
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references