Q97F65 (KITH_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	CA_C2887

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 195

195

Thymidine kinase HAMAP-Rule MF_00124

PRO_0000174967

Regions

Nucleotide binding

15 – 22

8

ATP HAMAP-Rule MF_00124

Nucleotide binding

57 – 58

2

ATP HAMAP-Rule MF_00124

Nucleotide binding

88 – 91

4

ATP By similarity

Sites

Active site

89

1

Proton acceptor Potential

Metal binding

145

1

Zinc

Metal binding

148

1

Zinc

Metal binding

183

1

Zinc

Metal binding

186

1

Zinc

Binding site

23

1

ATP

Binding site

120

1

Substrate; via amide nitrogen By similarity

Binding site

179

1

Substrate By similarity

Secondary structure

1

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195

Helix Strand Turn

Details...

Beta strand

9 – 14

6

Helix

21 – 34

14

Beta strand

39 – 44

6

Beta strand

54 – 56

3

Beta strand

62 – 64

3

Beta strand

66 – 71

6

Helix

73 – 77

5

Beta strand

83 – 87

5

Helix

90 – 92

3

Helix

97 – 106

10

Beta strand

110 – 115

6

Helix

128 – 134

7

Beta strand

136 – 140

5

Turn

146 – 148

3

Beta strand

149 – 152

4

Beta strand

154 – 159

6

Beta strand

177 – 182

6

Turn

184 – 186

3

Sequences

	Sequence		Length	Mass (Da)
	Q97F65 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 6EDB551986B203BA	FASTA	195	22,303
	10 20 30 40 50 60 MYRPKDHGWV EVIVGPMYSG KSEELIRRIR RAKIAKQKIQ VFKPEIDNRY SKEDVVSHMG 70 80 90 100 110 120 EKEQAVAIKN SREILKYFEE DTEVIAIDEV QFFDDEIVEI VNKIAESGRR VICAGLDMDF 130 140 150 160 170 180 RGKPFGPIPE LMAIAEFVDK IQAICVVCGN PATRTQRLIN GKPAFYDDPV VLIGAMESYE 190 ARCRKCHVVP QKKEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"X-ray structure of clostridium acetobutylicum genomics target CAR26." Northeast structural genomics consortium (NESG) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-191 IN COMPLEX WITH ZINC IONS AND ADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE001437 Genomic DNA. Translation: AAK80830.1.

PIR

C97255.

RefSeq

NP_349490.1. NC_003030.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XX6	X-ray	2.00	A/B	1-191	[»]

ProteinModelPortal

Q97F65.

SMR

Q97F65. Positions 2-191.

ModBase

Search...

Protein-protein interaction databases

STRING

272562.CA_C2887.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK80830; AAK80830; CA_C2887.

GeneID

1119070.

KEGG

cac:CA_C2887.

PATRIC

32040185. VBICloAce74127_3071.

Phylogenomic databases

eggNOG

COG1435.

HOGENOM

HOG000076390.

KO

K00857.

OMA

KVHAICV.

ProtClustDB

PRK04296.

Enzyme and pathway databases

BioCyc

CACE272562:GJIH-2970-MONOMER.

BRENDA

2.7.1.21. 1452.

Family and domain databases

HAMAP

MF_00124. Thymidine_kinase.

InterPro

IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]

PANTHER

PTHR11441. PTHR11441. 1 hit.

Pfam

PF00265. TK. 1 hit.
[Graphical view]

PIRSF

PIRSF035805. TK_cell. 1 hit.

PROSITE

PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q97F65.

Entry information

Entry name

KITH_CLOAB

Accession

Primary (citable) accession number: Q97F65

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program