Q97EZ2 (LACG_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	CA_C2963

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260722
Sites
	Active site	160	1	Proton donor By similarity
	Active site	374	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97EZ2 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 5661CA39AAC5C3BE	FASTA	474	54,672
	10 20 30 40 50 60 MNNFGEDFIF GGATAAYQAE GATKEDGKGP CIWDEYLKKE GRFTGDTASD FYHKYKEDLK 70 80 90 100 110 120 FSRKFGVNGI RISIAWSRVI PDGKGEVNPK GLKFYSDLID ECIKNNVEPF VTLHHFDTPL 130 140 150 160 170 180 TLFKDGDWLN RNNIDYFVRF AKVCFEALGD RVKKWITFNE AWAVAQNGYI IGNFPPSIKY 190 200 210 220 230 240 DIPKAAQSMH NMMVAHAKVV ELYKSMNLDG EIGIVHTLEG KYPITDSKED KEAAYLDYMI 250 260 270 280 290 300 SNKFMLDACF KGEYPKETEK TINEIMSKNG GELKIYDGDL EVLKKASSKI DFLGMNYYSS 310 320 330 340 350 360 HFLKAYEGES RIHHNGTGEK GTSIFALKGI GERVNNPEVP TTDWDWPIYP KGLHDMLVRI 370 380 390 400 410 420 KNEYPNYKKI YVTENGMGYK DDFKDGKIDD TPRIDYINKH LEAILKAKNE GVVVKGYFVW 430 440 450 460 470 SLMDVLSWSN GYNKRYGLFY VDFKTQNRYA KKSAYWFKRI SYEKKLIDFS DIEY

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK80905.1.
PIR	F97264.
RefSeq	NP_349565.1. NC_003030.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
ProteinModelPortal	Q97EZ2.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119146.
GenomeReviews	Gene locus CA_C2963 in contig AE001437_GR.
KEGG	cac:CA_C2963.
NMPDR	fig|272562.1.peg.3094.
PATRIC	32040337. VBICloAce74127_3147.
Phylogenomic databases
HOGENOM	HBG316462.
OMA	GMGYKDE.
PhylomeDB	Q97EZ2.
ProtClustDB	PRK13511.
Enzyme and pathway databases
BioCyc	CACE272562:CAC2963-MONOMER.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR005928. 6P-beta-galactosidase. IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01220.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. LacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_CLOAB

Accession

Primary (citable) accession number: Q97EZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program