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Q97EZ2 (LACG_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-galactosidase

EC=3.2.1.85
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG
Gene names
Name:lacG
Ordered Locus Names:CA_C2963
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP-Rule MF_01574

Pathway

Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlactose catabolic process via tagatose-6-phosphate

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phospho-beta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4744746-phospho-beta-galactosidase HAMAP-Rule MF_01574
PRO_0000260722

Sites

Active site1601Proton donor By similarity
Active site3741Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97EZ2 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 5661CA39AAC5C3BE

FASTA47454,672
        10         20         30         40         50         60 
MNNFGEDFIF GGATAAYQAE GATKEDGKGP CIWDEYLKKE GRFTGDTASD FYHKYKEDLK 

        70         80         90        100        110        120 
FSRKFGVNGI RISIAWSRVI PDGKGEVNPK GLKFYSDLID ECIKNNVEPF VTLHHFDTPL 

       130        140        150        160        170        180 
TLFKDGDWLN RNNIDYFVRF AKVCFEALGD RVKKWITFNE AWAVAQNGYI IGNFPPSIKY 

       190        200        210        220        230        240 
DIPKAAQSMH NMMVAHAKVV ELYKSMNLDG EIGIVHTLEG KYPITDSKED KEAAYLDYMI 

       250        260        270        280        290        300 
SNKFMLDACF KGEYPKETEK TINEIMSKNG GELKIYDGDL EVLKKASSKI DFLGMNYYSS 

       310        320        330        340        350        360 
HFLKAYEGES RIHHNGTGEK GTSIFALKGI GERVNNPEVP TTDWDWPIYP KGLHDMLVRI 

       370        380        390        400        410        420 
KNEYPNYKKI YVTENGMGYK DDFKDGKIDD TPRIDYINKH LEAILKAKNE GVVVKGYFVW 

       430        440        450        460        470 
SLMDVLSWSN GYNKRYGLFY VDFKTQNRYA KKSAYWFKRI SYEKKLIDFS DIEY 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK80905.1.
PIRF97264.
RefSeqNP_349565.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97EZ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C2963.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK80905; AAK80905; CA_C2963.
GeneID1119146.
KEGGcac:CA_C2963.
PATRIC32040337. VBICloAce74127_3147.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088632.
KOK01220.
OMAANESIPR.
OrthoDBEOG60658J.

Enzyme and pathway databases

BioCycCACE272562:GJIH-3046-MONOMER.
UniPathwayUPA00542; UER00605.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
HAMAPMF_01574. LacG.
InterProIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01233. lacG. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACG_CLOAB
AccessionPrimary (citable) accession number: Q97EZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries