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Protein

Thymidylate synthase

Gene

thyA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25dUMPUniRule annotation1
Active sitei143NucleophileUniRule annotation1
Binding sitei1665,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei174dUMPUniRule annotation1
Binding sitei2625,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 124dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi163 – 166dUMPUniRule annotation4
Nucleotide bindingi204 – 206dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:CA_C3003
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409491 – 263Thymidylate synthaseAdd BLAST263

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272562.CA_C3003

Structurei

3D structure databases

ProteinModelPortaliQ97EV3
SMRiQ97EV3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q97EV3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKADEKYLD IIENILENGY YDNNRTGIET YKLPHQIMQF DLSKEFPILT
60 70 80 90 100
TKFVAFKTSV KELLWIYKDQ SNDVRKLQNE NVTIWDEWML EDGTIGKAYG
110 120 130 140 150
YQVAKFKQID NLIDTLKTDP QSRRMLISLW NIADLKDMSL TPCCYETLWD
160 170 180 190 200
VQNGKLNCML VQRSGDMPLG VPFNMCQYAV LVHLIAQVTG LKPGLFTHVI
210 220 230 240 250
NNAHIYKNQI EGMKIQLKRR DKAYEAPKLW INPNIKNFYD FTADDIKLID
260
YKHHDKIKME VAV
Length:263
Mass (Da):30,769
Last modified:October 1, 2001 - v1
Checksum:i833EABDB1E94EF6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK80944.1
PIRiE97269
RefSeqiNP_349604.1, NC_003030.1
WP_010966285.1, NC_003030.1

Genome annotation databases

EnsemblBacteriaiAAK80944; AAK80944; CA_C3003
GeneIDi1119186
KEGGicac:CA_C3003
PATRICifig|272562.8.peg.3187

Similar proteinsi

Entry informationi

Entry nameiTYSY_CLOAB
AccessioniPrimary (citable) accession number: Q97EV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2001
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health