Q97EV1 (ADD_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine deaminase
EC=3.5.4.4

Alternative name(s):
Adenosine aminohydrolase

Gene names

Name:	add
Ordered Locus Names:	CA_C3005

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Adenosine + H2O = inosine + NH3. HAMAP MF_00540
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00540
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenosine deaminase subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Adenosine deaminase HAMAP MF_00540		PRO_0000194365
Sites
	Active site	203	1	Proton donor By similarity
	Metal binding	16	1	Zinc; catalytic By similarity
	Metal binding	18	1	Zinc; catalytic By similarity
	Metal binding	200	1	Zinc; catalytic By similarity
	Metal binding	281	1	Zinc; catalytic By similarity
	Binding site	18	1	Substrate By similarity
	Binding site	20	1	Substrate By similarity
	Binding site	173	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Site	224	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97EV1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: FC386C9C80DC789B	FASTA	334	38,023
	10 20 30 40 50 60 MNIKEQIINL PKVELHCHLD GSLRPETVLD LCLKENINIP YENPEDFKSS LKISKNCSSL 70 80 90 100 110 120 KEYLEKFYFP IRVMQKKENI YRVTMELLED SKKDGIEYTE IRFAPFQHTE QDLNENDVVE 130 140 150 160 170 180 AALEALQDGE SKLGIHSNLI LCSLRHDPVE RSIDLVNLAN SYNEGVCAVD LAGNESDFPP 190 200 210 220 230 240 ELHKEAFDLA YDNGIKITIH AGETGIAENI LKSIKLLHAD RIGHGIFAYK SEEILQYVIE 250 260 270 280 290 300 NQVPLEMCPK SNVDTKAVKN YKNHPFKKYF DLGVKVTLNT DNRTVSNVSL VDEYLNLANI 310 320 330 FDFGIEEIKT VIRNGISASF ATEEFKVNLL KKLD

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK80946.1.
PIR	G97269.
RefSeq	NP_349606.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97EV1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119188.
GenomeReviews	Gene locus CA_C3005 in contig AE001437_GR.
KEGG	cac:CA_C3005.
NMPDR	fig|272562.1.peg.3135.
PATRIC	32040421. VBICloAce74127_3189.
Phylogenomic databases
HOGENOM	HBG630382.
OMA	ITLECCA.
PhylomeDB	Q97EV1.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3005-MONOMER.
Family and domain databases
HAMAP	MF_00540. A_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
PROSITE	PS00485. A_DEAMINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADD_CLOAB

Accession

Primary (citable) accession number: Q97EV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program