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Q97ES0

- SYI_CLOAB

UniProt

Q97ES0 - SYI_CLOAB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei592 – 5921ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-3121-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CA_C3038
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10351035Isoleucine--tRNA ligasePRO_0000098533Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272562.CA_C3038.

    Structurei

    3D structure databases

    ProteinModelPortaliQ97ES0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi589 – 5935"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q97ES0-1 [UniParc]FASTAAdd to Basket

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    MYKKVDSSKS FVDIERDVLK LWHEKEIVKK SFNSNQDGEY FSFYDGPPTA     50
    NGRPHVGHII TRVMKDLIPR YKVMKGYKVP RKAGWDTHGL PVELEIEKKL 100
    GISGKPQIEE YGIEKFVKEC KESVFSYVSL WKDMSEKLGY WVDMENPYVT 150
    YHNDYIESVW WALKQMWDKD LLYKGHKIVP YCPRCGTALS SHEVAQGYKD 200
    VKEATAFVKF KVKGEENKYI LAWTTTPWTL PSNVALAINK AYDYVEVINN 250
    GEHLILAKAL LTVLEGEYEV VSEFKGEKLL GMEYEQLFKF ANPDKKAFYV 300
    VHGDFVTLSD GTGIVHIAPA YGEDDNMLGK KYDLPLINLV NGEGKFVDEV 350
    EPWKGLFVKK ADPKILEYMK ENGTLYKSEK FTHSYPHCWR CDTPLLYYPR 400
    DSWFVRMTSL RDKLVENNNK IHWYPDNIRT GRFGKFVENV IDWGISRDRY 450
    WGTPLPIWQC ECGHRDCVGS IEELKEKGIN VPENIELHKP YIDEVKLTCP 500
    KCGKPMTRTE EVIDCWFDSG SMPFAQLHYP FENKEVFENT FPAQFISEAV 550
    DQTRGWFYTL LAISTSIFDK SSFENCIVLG HVLDKHGLKM SKHKGNVVDP 600
    FDVLDNQGAD ACRWHFYTSS APWLPTRFSQ EDVAETQRKF LSTLWNVYSF 650
    YVLYAEIDKF NPNDYKDFVS GNVMDKWIVS RLNTLTKDVG NYLDSYGITQ 700
    AALEIQDFVD DLSNWYVRRN RSRYWTQELT DDKVGAYVTL YRVLVTLSKV 750
    AAPFIPFMTE QIYQSLVTSI NEGAEESVHL CKWPEYDESL IDSSLEEEMK 800
    LAISIVKLGR SARNGANIKN RQPLYEMRVS TKALPDYYGD IIRDELNVKK 850
    VEFGADLSKY VNFEIKPNLP VLGKSYGKLI PKIRKEISSM DQMKLAERVR 900
    SGEAVKIDVD GTEIELNSEN LLVTMQGLEG FAFAGIGEIG IVLETTITDE 950
    LREEGYLREV LSKVQNMRKE SGFEVADKIE IYVSGNEKLE NVIRKFEDTI 1000
    KKETLATDII YSENKEAAIY NINGEELNVF VKKNC 1035
    Length:1,035
    Mass (Da):119,411
    Last modified:October 1, 2001 - v1
    Checksum:i5FC26B022AFDAA86
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK80978.1.
    PIRiG97273.
    RefSeqiNP_349638.1. NC_003030.1.

    Genome annotation databases

    EnsemblBacteriaiAAK80978; AAK80978; CA_C3038.
    GeneIDi1119221.
    KEGGicac:CA_C3038.
    PATRICi32040485. VBICloAce74127_3221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE001437 Genomic DNA. Translation: AAK80978.1 .
    PIRi G97273.
    RefSeqi NP_349638.1. NC_003030.1.

    3D structure databases

    ProteinModelPortali Q97ES0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_C3038.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK80978 ; AAK80978 ; CA_C3038 .
    GeneIDi 1119221.
    KEGGi cac:CA_C3038.
    PATRICi 32040485. VBICloAce74127_3221.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-3121-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiSYI_CLOAB
    AccessioniPrimary (citable) accession number: Q97ES0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3