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Protein

Phosphoenolpyruvate-protein phosphotransferase

Gene

CA_C3087

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei188 – 1881Tele-phosphohistidine intermediateUniRule annotation
Binding sitei295 – 2951SubstrateUniRule annotation
Binding sitei331 – 3311SubstrateUniRule annotation
Metal bindingi430 – 4301MagnesiumUniRule annotation
Binding sitei430 – 4301SubstrateUniRule annotation
Binding sitei451 – 4511Substrate; via carbonyl oxygenUniRule annotation
Binding sitei452 – 4521Substrate; via amide nitrogenUniRule annotation
Binding sitei453 – 4531SubstrateUniRule annotation
Metal bindingi454 – 4541MagnesiumUniRule annotation
Binding sitei454 – 4541Substrate; via amide nitrogenUniRule annotation
Active sitei501 – 5011Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

Phosphotransferase systemUniRule annotation, Sugar transportUniRule annotation, Transport

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, PyruvateImported

Enzyme and pathway databases

BioCyciCACE272562:GJIH-3170-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate-protein phosphotransferaseUniRule annotation (EC:2.7.3.9UniRule annotation)
Alternative name(s):
Phosphotransferase system, enzyme IUniRule annotation
Gene namesi
Ordered Locus Names:CA_C3087Imported
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)Imported
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiQ97EM3.

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_C3087.

Structurei

3D structure databases

ProteinModelPortaliQ97EM3.
SMRiQ97EM3. Positions 4-538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 125122PEP-utilisers_NInterPro annotationAdd
BLAST
Domaini151 – 22474PEP-utilizersInterPro annotationAdd
BLAST
Domaini251 – 538288PEP-utilizers_CInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 5935Sequence analysisAdd
BLAST
Coiled coili228 – 26235Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
HOGENOMiHOG000278513.
KOiK08483.
OMAiNIEIGIM.

Family and domain databases

Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR024692. PTS_EI.
IPR006318. PTS_EI-like.
IPR008731. PTS_EIN.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000732. PTS_enzyme_I. 1 hit.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97EM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKGISASK GYAIGHVFIK ADNEVKIVEK KISDIESEKA RLQGAVEKAR
60 70 80 90 100
EQLTKIKEKA EKDLGADKAA VFESHMMFLD DPDFIGSAEN IISDEMINAE
110 120 130 140 150
KALENVMKNY VAIFEGIEDE YVKERIADVK DVGNRVLQNL AGNDMTSLAD
160 170 180 190 200
VDNNTVVVAH DLTPSDTAQL DKNRVIGFLT NIGGRTSHSA IMARTLEIPA
210 220 230 240 250
VVGLQDIVES VKNGDTIVVD GAEGIAIINP DEATLKEYEA KKAEYEKKQE
260 270 280 290 300
KLRELINVET RTKEGKRVEV CGNIGKAKDV EAVLQNGGDG VGLFRTEFLY
310 320 330 340 350
MDRDQMPTED EQFEAYKAVV EKMGEKPVVI RTLDIGGDKK LPYLPLPEEM
360 370 380 390 400
NPFLGYRAIR LCLGRKDIFK IQLRALLRAS VYGNLKIMFP MISSLEEFLS
410 420 430 440 450
AKEVLKECMG ELDKEGKKYN AKLETGIMVE IPAAAVNSEE LAKYVDFFSI
460 470 480 490 500
GTNDLIQYTL AADRMNEKVS YLYNPMHPAV LKLIKMTIQS AHKEGKWCGM
510 520 530
CGEMAGDEKA IPTLVEYGLD EFSMSASSIL TAKELIMKA
Length:539
Mass (Da):59,821
Last modified:October 1, 2001 - v1
Checksum:i4BB5A112FE38397C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK81027.1.
PIRiH97279.
RefSeqiNP_349687.1. NC_003030.1.
WP_010966368.1. NC_003030.1.

Genome annotation databases

EnsemblBacteriaiAAK81027; AAK81027; CA_C3087.
GeneIDi1119270.
KEGGicac:CA_C3087.
PATRICi32040583. VBICloAce74127_3270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA. Translation: AAK81027.1.
PIRiH97279.
RefSeqiNP_349687.1. NC_003030.1.
WP_010966368.1. NC_003030.1.

3D structure databases

ProteinModelPortaliQ97EM3.
SMRiQ97EM3. Positions 4-538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_C3087.

Proteomic databases

PRIDEiQ97EM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK81027; AAK81027; CA_C3087.
GeneIDi1119270.
KEGGicac:CA_C3087.
PATRICi32040583. VBICloAce74127_3270.

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
HOGENOMiHOG000278513.
KOiK08483.
OMAiNIEIGIM.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-3170-MONOMER.

Family and domain databases

Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR024692. PTS_EI.
IPR006318. PTS_EI-like.
IPR008731. PTS_EIN.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000732. PTS_enzyme_I. 1 hit.
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ97EM3_CLOAB
AccessioniPrimary (citable) accession number: Q97EM3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2001
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.