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Protein

Phosphoenolpyruvate-protein phosphotransferase

Gene

CA_C3087

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei188Tele-phosphohistidine intermediateUniRule annotation1
Binding sitei295PEPUniRule annotation1
Binding sitei331PEPUniRule annotation1
Metal bindingi430MagnesiumUniRule annotation1
Metal bindingi454MagnesiumUniRule annotation1
Binding sitei464PEPUniRule annotation1
Active sitei501Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinaseUniRule annotationImported, Transferase
Biological processPhosphotransferase systemUniRule annotation, Sugar transportUniRule annotation, Transport
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, PyruvateImported

Enzyme and pathway databases

BioCyciCACE272562:GJIH-3053-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate-protein phosphotransferaseUniRule annotation (EC:2.7.3.9UniRule annotation)
Alternative name(s):
Phosphotransferase system, enzyme IUniRule annotation
Gene namesi
Ordered Locus Names:CA_C3087Imported
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)Imported
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000814 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi272562.CA_C3087

Structurei

3D structure databases

ProteinModelPortaliQ97EM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 125PEP-utilisers_NInterPro annotationAdd BLAST122
Domaini151 – 224PEP-utilizersInterPro annotationAdd BLAST74
Domaini251 – 538PEP-utilizers_CInterPro annotationAdd BLAST288

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni453 – 454PEP bindingUniRule annotation2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 59Sequence analysisAdd BLAST35
Coiled coili228 – 262Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105BZ3 Bacteria
COG1080 LUCA
HOGENOMiHOG000278513
KOiK08483
OMAiCNAEWAL

Family and domain databases

Gene3Di1.10.274.10, 1 hit
InterProiView protein in InterPro
IPR008279 PEP-util_enz_mobile_dom
IPR018274 PEP_util_AS
IPR000121 PEP_util_C
IPR023151 PEP_util_CS
IPR036637 Phosphohistidine_dom_sf
IPR024692 PTS_EI
IPR006318 PTS_EI-like
IPR008731 PTS_EIN
IPR036618 PtsI_HPr-bd_sf
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PfamiView protein in Pfam
PF05524 PEP-utilisers_N, 1 hit
PF00391 PEP-utilizers, 1 hit
PF02896 PEP-utilizers_C, 1 hit
PIRSFiPIRSF000732 PTS_enzyme_I, 1 hit
SUPFAMiSSF47831 SSF47831, 1 hit
SSF51621 SSF51621, 1 hit
SSF52009 SSF52009, 1 hit
TIGRFAMsiTIGR01417 PTS_I_fam, 1 hit
PROSITEiView protein in PROSITE
PS00742 PEP_ENZYMES_2, 1 hit
PS00370 PEP_ENZYMES_PHOS_SITE, 1 hit

Sequencei

Sequence statusi: Complete.

Q97EM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKGISASK GYAIGHVFIK ADNEVKIVEK KISDIESEKA RLQGAVEKAR
60 70 80 90 100
EQLTKIKEKA EKDLGADKAA VFESHMMFLD DPDFIGSAEN IISDEMINAE
110 120 130 140 150
KALENVMKNY VAIFEGIEDE YVKERIADVK DVGNRVLQNL AGNDMTSLAD
160 170 180 190 200
VDNNTVVVAH DLTPSDTAQL DKNRVIGFLT NIGGRTSHSA IMARTLEIPA
210 220 230 240 250
VVGLQDIVES VKNGDTIVVD GAEGIAIINP DEATLKEYEA KKAEYEKKQE
260 270 280 290 300
KLRELINVET RTKEGKRVEV CGNIGKAKDV EAVLQNGGDG VGLFRTEFLY
310 320 330 340 350
MDRDQMPTED EQFEAYKAVV EKMGEKPVVI RTLDIGGDKK LPYLPLPEEM
360 370 380 390 400
NPFLGYRAIR LCLGRKDIFK IQLRALLRAS VYGNLKIMFP MISSLEEFLS
410 420 430 440 450
AKEVLKECMG ELDKEGKKYN AKLETGIMVE IPAAAVNSEE LAKYVDFFSI
460 470 480 490 500
GTNDLIQYTL AADRMNEKVS YLYNPMHPAV LKLIKMTIQS AHKEGKWCGM
510 520 530
CGEMAGDEKA IPTLVEYGLD EFSMSASSIL TAKELIMKA
Length:539
Mass (Da):59,821
Last modified:October 1, 2001 - v1
Checksum:i4BB5A112FE38397C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001437 Genomic DNA Translation: AAK81027.1
PIRiH97279
RefSeqiNP_349687.1, NC_003030.1
WP_010966368.1, NC_003030.1

Genome annotation databases

EnsemblBacteriaiAAK81027; AAK81027; CA_C3087
GeneIDi1119270
KEGGicac:CA_C3087
PATRICifig|272562.8.peg.3270

Similar proteinsi

Entry informationi

Entry nameiQ97EM3_CLOAB
AccessioniPrimary (citable) accession number: Q97EM3
Entry historyiIntegrated into UniProtKB/TrEMBL: October 1, 2001
Last sequence update: October 1, 2001
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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