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Q97EL4 (THIM_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:CA_C3096
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000156928

Sites

Binding site411Substrate; via amide nitrogen By similarity
Binding site1171ATP By similarity
Binding site1701ATP By similarity
Binding site1971Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97EL4 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 32CCB7DAD5C6010E

FASTA27329,349
        10         20         30         40         50         60 
MLKERLENVR GTNPLIHNIT NYVTVNDCAN ILLACGASPI MADDIGEVEE ITSICRGLNI 

        70         80         90        100        110        120 
NIGTLNENTI ESMLKAGKMA NKLKHPVILD PVGVGASKLR RQTALKLLEE VKFTVIRGNI 

       130        140        150        160        170        180 
SEIKVLALGQ GITKGVDADV NDKVKDDTLD NVINFAKGFS KKTGAVIAIT GAIDIIANES 

       190        200        210        220        230        240 
KAYVVRNGNP NMSKITGTGC MLSAMTAAYI SANVDSPLEA TLASVCAMGI CGELAYNRLS 

       250        260        270 
KRDGNSSYRN YIIDEVFNLD GDKLDDAARY ESY 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK81036.1.
PIRA97281.
RefSeqNP_349696.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97EL4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C3096.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK81036; AAK81036; CA_C3096.
GeneID1119279.
KEGGcac:CA_C3096.
PATRIC32040601. VBICloAce74127_3279.

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMALPLMATH.
OrthoDBEOG628F8M.
ProtClustDBPRK09355.

Enzyme and pathway databases

BioCycCACE272562:GJIH-3179-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
ProtoNetSearch...

Entry information

Entry nameTHIM_CLOAB
AccessionPrimary (citable) accession number: Q97EL4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2001
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways