Q97EB3 (FTHS_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	CA_C3201

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_0000199337
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97EB3 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 7B0629B679B832FE	FASTA	556	60,507
	10 20 30 40 50 60 MKTDIEIAQE AKMEPIVKIA EKIGLNEDDI DLYGKYKCKI SLDVLKQNKN KQDGKLVLVT 70 80 90 100 110 120 AINPTPAGEG KSTVTVGLGE ALCKMNKNTV IALREPSLGP VFGIKGGAAG GGYAQVVPME 130 140 150 160 170 180 DINLHFTGDM HAITSANNLL CAAIDNHIHQ GNSLKIDQRR IVFKRVMDMN DRALRSIVVG 190 200 210 220 230 240 LGGKVNGFPR EDGFMITVAS EIMAILCLAN DLMDLKERMG KILIAYDLDG NPVYCRDLKV 250 260 270 280 290 300 EGAMAMLMKD AMKPNLVQTL ENTPAIIHGG PFANIAHGCN SILATKMALK LGDYVITEAG 310 320 330 340 350 360 FGADLGAEKF LDIKCRYGNL NPDCVVLVAT IRALKHHGGA LKEDLSKPNA KVLEKGLSNL 370 380 390 400 410 420 GKQIENIKKY GVPVVVAINK FITDSEEEIK CIEEYCSKQG VKVSLTEVWE KGGEGGTDLA 430 440 450 460 470 480 NKVLDTLENE KSNFKYLYDE KLSIKEKMDI IAKEIYGADG VQYTPQANKQ IKEIEKFNLD 490 500 510 520 530 540 KLPICVAKTQ YSLSDNPALL GRPTNFTINV KEVRVSNGAG FVVVQTGNIM TMPGLPKTPA 550 ANKMDIFEDG SIVGLF

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81137.1.
PIR	F97293.
RefSeq	NP_349797.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97EB3.
SMR	Q97EB3. Positions 4-554.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119383.
GenomeReviews	Gene locus CA_C3201 in contig AE001437_GR.
KEGG	cac:CA_C3201.
NMPDR	fig|272562.1.peg.3326.
PATRIC	32040821. VBICloAce74127_3380.
Phylogenomic databases
HOGENOM	HBG677721.
OMA	EIMAVLC.
PhylomeDB	Q97EB3.
ProtClustDB	PRK13505.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3201-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_CLOAB

Accession

Primary (citable) accession number: Q97EB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program