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Q97EB3 (FTHS_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:CA_C3201
OrganismClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]
Taxonomic identifier272562 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_0000199337

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97EB3 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 7B0629B679B832FE

FASTA55660,507
        10         20         30         40         50         60 
MKTDIEIAQE AKMEPIVKIA EKIGLNEDDI DLYGKYKCKI SLDVLKQNKN KQDGKLVLVT 

        70         80         90        100        110        120 
AINPTPAGEG KSTVTVGLGE ALCKMNKNTV IALREPSLGP VFGIKGGAAG GGYAQVVPME 

       130        140        150        160        170        180 
DINLHFTGDM HAITSANNLL CAAIDNHIHQ GNSLKIDQRR IVFKRVMDMN DRALRSIVVG 

       190        200        210        220        230        240 
LGGKVNGFPR EDGFMITVAS EIMAILCLAN DLMDLKERMG KILIAYDLDG NPVYCRDLKV 

       250        260        270        280        290        300 
EGAMAMLMKD AMKPNLVQTL ENTPAIIHGG PFANIAHGCN SILATKMALK LGDYVITEAG 

       310        320        330        340        350        360 
FGADLGAEKF LDIKCRYGNL NPDCVVLVAT IRALKHHGGA LKEDLSKPNA KVLEKGLSNL 

       370        380        390        400        410        420 
GKQIENIKKY GVPVVVAINK FITDSEEEIK CIEEYCSKQG VKVSLTEVWE KGGEGGTDLA 

       430        440        450        460        470        480 
NKVLDTLENE KSNFKYLYDE KLSIKEKMDI IAKEIYGADG VQYTPQANKQ IKEIEKFNLD 

       490        500        510        520        530        540 
KLPICVAKTQ YSLSDNPALL GRPTNFTINV KEVRVSNGAG FVVVQTGNIM TMPGLPKTPA 

       550 
ANKMDIFEDG SIVGLF 

« Hide

References

[1]"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.
J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE001437 Genomic DNA. Translation: AAK81137.1.
PIRF97293.
RefSeqNP_349797.1. NC_003030.1.

3D structure databases

ProteinModelPortalQ97EB3.
SMRQ97EB3. Positions 4-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272562.CA_C3201.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK81137; AAK81137; CA_C3201.
GeneID1119383.
KEGGcac:CA_C3201.
PATRIC32040821. VBICloAce74127_3380.

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMALKHHGGV.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycCACE272562:GJIH-3293-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOAB
AccessionPrimary (citable) accession number: Q97EB3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways