Q97E93 (KPRS_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	CA_C3221

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141126
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	132	1	Magnesium Potential
	Metal binding	134	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q97E93 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: BF42608FBDBF4832	FASTA	319	34,862
	10 20 30 40 50 60 MINHGKNIKI FTGNSYPSLA EEIADIIGVQ VGDSRVGKFS NGETAVDINE TVRGTDLFLI 70 80 90 100 110 120 QTLCEPVNDS IMELLIMLDA FKRASAGRIT AVIPNYAYAR QDRKAKARQP ITAKLMADLI 130 140 150 160 170 180 HTAGADRVLT MDLHAPQIQG FFDIPVDHLE GVPILAKYFI NQNSNTDDLI VVSPDIGGVK 190 200 210 220 230 240 RTRKFAEKLH APIAIIDKRR PKPNVSEVMS IIGDVKGKKA ILVDDMIDTA GSIVNAAEAL 250 260 270 280 290 300 VKMGAKEVSA CCTHGVLSGP AIERLENSPL KEVVILNTIP IEGDKRIDKI KVLSVAPIFA 310 EAIRRIYEDM PVSKIFQEE

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81157.1.
PIR	B97296.
RefSeq	NP_349817.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97E93.
SMR	Q97E93. Positions 7-317.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119403.
GenomeReviews	Gene locus CA_C3221 in contig AE001437_GR.
KEGG	cac:CA_C3221.
NMPDR	fig|272562.1.peg.3346.
PATRIC	32040867. VBICloAce74127_3400.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	TRISPTE.
PhylomeDB	Q97E93.
ProtClustDB	CLSK2751989.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3221-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_CLOAB

Accession

Primary (citable) accession number: Q97E93

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program