Reviewed, UniProtKB/Swiss-Prot Q97E86 (NAMA_CLOAB)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	CA_C3229

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	NADPH dehydrogenase HAMAP MF_01614		PRO_0000216120
Regions
	Nucleotide binding	21 – 25	5	FMN By similarity
Sites
	Binding site	26	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97E86-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 5958453FA93F2EDF	FASTA	339	37,677
	10 20 30 40 50 60 MKIFEAYTIK NMCLKNRIVM PPMCMYSSDN TGNINDFHLV HYTTRSIGGV GFIIVEATGI 70 80 90 100 110 120 TPNGRISDKD LGIWSEKHAE GLSFLVKEVK KYGSKIAIQL NHSGRKYEGT SGEPVAPSAL 130 140 150 160 170 180 AFDENSKTPK ELTKNEIKEI ILAFKAAAKR AEKAGFDAIE IHGAHGYLIN QFLSPLSNFR 190 200 210 220 230 240 DDEYGGSTEN RTRFLKEVLE AVREVWPKEK PILLRVSAED YRGGSGITPN EMVNIINIVK 250 260 270 280 290 300 DLIDVVDVSS GGVAPAHINL YPGYQVKLSE VIKNECNVPT IAVGLICDIN MVEEILSNNR 310 320 330 ADLVALGREL LRNPYFVLSS ARLKNINIDF PKPYERAFN

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001437 Genomic DNA. Translation: AAK81164.1.
PIR	A97297.
RefSeq	NP_349824.1.
3D structure databases
HSSP	HSSP built from PDB template 1OYC based on UniProtKB Q02899.
ModBase	Search...
Genome annotation databases
GeneID	1119411.
GenomeReviews	Gene locus CA_C3229 in contig AE001437_GR.
KEGG	cac:CAC3229.
NMPDR	fig|272562.1.peg.3353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97E86.
OMA	Q97E86. RGLTVRN.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3229-MON.
BRENDA	1.6.99.1. 2866.
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_CLOAB

Accession

Primary (citable) accession number: Q97E86

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2001
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)