Reviewed, UniProtKB/Swiss-Prot Q97E82 (COMB_CLOAB)

Names and origin

Protein names

Recommended name:
    Probable 2-phosphosulfolactate phosphatase
    EC=3.1.3.71

Gene names

Name:	comB
Ordered Locus Names:	CA_C3233

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(2R)-2-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate. HAMAP MF_00490
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the comB family.

Ontologies

Keywords
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	coenzyme M biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	2-phosphosulfolactate phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 235

235

Probable 2-phosphosulfolactate phosphatase HAMAP MF_00490

PRO_0000081465

Secondary structure

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235

Helix Strand Turn

Details...

Beta strand

1 – 6

6

Helix

9 – 11

3

Helix

14 – 16

3

Turn

17 – 19

3

Beta strand

20 – 25

6

Turn

27 – 29

3

Helix

30 – 39

10

Beta strand

44 – 47

4

Helix

51 – 61

11

Helix

62 – 64

3

Beta strand

65 – 69

5

Helix

85 – 87

3

Helix

90 – 93

4

Beta strand

97 – 101

5

Helix

105 – 111

7

Turn

112 – 114

3

Beta strand

115 – 121

7

Helix

126 – 136

11

Beta strand

140 – 144

5

Helix

153 – 169

17

Beta strand

173 – 175

3

Helix

177 – 187

11

Helix

193 – 196

4

Helix

200 – 207

8

Helix

211 – 217

7

Beta strand

228 – 230

3

Sequences

	Sequence		Length	Mass (Da)
	Q97E82-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: A8BFB7DF07161EBD	FASTA	235	26,144
	10 20 30 40 50 60 MKIDLIISAD DIKEEKVKNK TAVVIDMLRA TSVITTALNN GCKRVVPVLT VEEALKKVKE 70 80 90 100 110 120 YGKDAILGGE RKGLKIEGFD FSNSPMEYTE DVVKGKTLIM TTTNGTRAIK GSETARDILI 130 140 150 160 170 180 GSVLNGEAVA EKIVELNNDV VIVNAGTYGE FSIDDFICSG YIINCVMDRM KKLELTDAAT 190 200 210 220 230 TAQYVYKTNE DIKGFVKYAK HYKRIMELGL KKDFEYCCKK DIVKLVPQYT NGEIL

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE001437 Genomic DNA. Translation: AAK81168.1.

PIR

E97297.

RefSeq

NP_349828.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VR0	X-ray	2.49	A/B/C	1-235	[»]

ModBase

Search...

Genome annotation databases

GeneID

1119415.

GenomeReviews

Gene locus CA_C3233 in contig AE001437_GR.

KEGG

cac:CAC3233.

NMPDR

fig|272562.1.peg.3357.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q97E82.

OMA

Q97E82. DFICSGY.

Enzyme and pathway databases

BioCyc

CACE272562:CAC3233-MON.

BRENDA

3.1.3.71. 2866.

Family and domain databases

HAMAP

MF_00490.
[Tree]

InterPro

IPR005238. 2-PSlactate_phosphatase.
[Graphical view]

Gene3D

G3DSA:3.90.1560.10. 2-PSlactate_phosphatase. 1 hit.

Pfam

PF04029. 2-ph_phosp. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

COMB_CLOAB

Accession

Primary (citable) accession number: Q97E82

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	October 1, 2001
Last modified:	May 5, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)