Reviewed, UniProtKB/Swiss-Prot Q97E66 (MURI_CLOAB)

Names and origin

Protein names

Recommended name:
    Glutamate racemase
    EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	CA_C3250

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Glutamate racemase HAMAP MF_00258		PRO_0000095465

Sequences

	Sequence		Length	Mass (Da)
	Q97E66-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 25826B159987253C	FASTA	256	28,703
	10 20 30 40 50 60 MSVKNNPIGV IDSGVGGISV LKEAVKQLPY ENFIYYGDSK NAPYGIKSVD EVRNLTFNVV 70 80 90 100 110 120 EKLLKLNIKA LVVACNTATS AAIDELREKY KNIPVIGIEP ALKPAVELKR RGKIIIMATP 130 140 150 160 170 180 MTLSEVKFKN LMEKYEKDSE IVKLPCPGLV ELIEDGIVEG EKMQEYLRNK FKDYEKDDIA 190 200 210 220 230 240 AFVLGCTHYP FVKKEIASIA RDVPIIDGSQ GTVMQLKRKL DKYDILNIGS EKGKIKIMNS 250 LKDDKILDLS HRLLDL

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81184.1.
PIR	E97299.
RefSeq	NP_349844.1.
3D structure databases
SMR	Q97E66. Positions 5-255.
ModBase	Search...
Genome annotation databases
GeneID	1119432.
GenomeReviews	Gene locus CA_C3250 in contig AE001437_GR.
KEGG	cac:CAC3250.
NMPDR	fig|272562.1.peg.3373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG645102.
OMA	LPCPGLV.
PhylomeDB	Q97E66.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3250-MONOMER.
BRENDA	5.1.1.3. 2866.
Family and domain databases
HAMAP	MF_00258. Glu_racemase. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
TIGRFAMs	TIGR00067. glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_CLOAB

Accession

Primary (citable) accession number: Q97E66

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)