Q97E56 (SYN_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	CA_C3260

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176401

Sequences

	Sequence		Length	Mass (Da)
	Q97E56 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: BC70BD197D4ADB43	FASTA	463	53,440
	10 20 30 40 50 60 MENVLVKQLY RLSSEFGGKK VRLSGWVRTI RASKSFGFIE INDGSFFKNI QVVFDDKLDN 70 80 90 100 110 120 FKEISKFIIS STITVEGEFV LTPNAKQPFE IHAENITLEG NSDNDYPLQK KRHTLEYLRS 130 140 150 160 170 180 IAHLRPRSNT FSAVFRVRSL AAYAVHKFFQ ERDFVYVNTP LITASDAEGA GEMFQVTTLD 190 200 210 220 230 240 LKNPPKNEEG NIDFSKDFFG KKANLTVSGQ LSAETFALAF RNVYTFGPTF RAEESNTTRH 250 260 270 280 290 300 AAEFWMIEPE MAFAELSDYL DTAEEMVKYI INFVMENAPE EMEFFNTRID KGLFDRLHNV 310 320 330 340 350 360 VNSEFGRITY TEAVDILEKS GEKFEYPVKW GIDLQTEHER YLTDKVFKKP LFVTDYPKDI 370 380 390 400 410 420 KAFYMRINDD NKTVAAADLL VPGVGEIIGG SQREERLAVL EKRMEELNLN KEDYWWYLEL 430 440 450 460 RKYGETKHSG YGLGFERMIM YLTGISNIRD VIPFPRTTGS AEF

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81194.1.
PIR	G97300.
RefSeq	NP_349854.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97E56.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119442.
GenomeReviews	Gene locus CA_C3260 in contig AE001437_GR.
KEGG	cac:CA_C3260.
NMPDR	fig|272562.1.peg.3383.
PATRIC	32040947. VBICloAce74127_3438.
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	Q97E56.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3260-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_CLOAB

Accession

Primary (citable) accession number: Q97E56

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program