Reviewed, UniProtKB/Swiss-Prot Q97DY9 (ALR2_CLOAB)

Names and origin

Protein names

Recommended name:
    Alanine racemase 2
    EC=5.1.1.1

Gene names

Name:	alr2
Ordered Locus Names:	CA_C3331

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Alanine racemase 2 HAMAP MF_01201		PRO_0000114510
Sites
	Active site	60	1	Proton acceptor; specific for D-alanine By similarity
	Active site	288	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	60	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97DY9-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 1A13F287C5C7CE43	FASTA	395	44,613
	10 20 30 40 50 60 MEKKTVYNIT NSIAISEEEA VNCNIPEVNK AEINLSQLKR NFDIIQGFLK PNTKFMAVLK 70 80 90 100 110 120 GDAYGHGIRP IAKELINLKC DVFGVVRLIE AFTLRKAGIK TPIMLLAPIS PSQAAWVIRY 130 140 150 160 170 180 NIIPMVDSEE IVEALDQSAS QNDTIVKLHV KINTGLNRYG VNPENAVKFI REIHEKYLHV 190 200 210 220 230 240 QVDGVYTHFQ DPDYNPDFTH KQIECFNNII FQLQKQKLRP RIIHAANSTG IIRYPEAHYD 250 260 270 280 290 300 MVRCGTLLFG LEHEQGQRNM PKGIKTLMEL KGRIMKVRTI RAGEAGGYGS TFVAKKDSKV 310 320 330 340 350 360 AIIAFGYGDG ISRGWKEVLV AGKRVPVVNY FMDGLMVDIS NIDETVKELD EAVIVGNQGD 370 380 390 ESITWLEACK SLGSYVDEQI QCITERVPKK YFYEK

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001437 Genomic DNA. Translation: AAK81263.1.
PIR	D97309.
RefSeq	NP_349923.1.
3D structure databases
HSSP	HSSP built from PDB template 1BD0 based on UniProtKB P10724.
ModBase	Search...
Genome annotation databases
GeneID	1119513.
GenomeReviews	Gene locus CA_C3331 in contig AE001437_GR.
KEGG	cac:CAC3331.
NMPDR	fig|272562.1.peg.3452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97DY9.
OMA	AYHYGLE.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3331-MON.
BRENDA	5.1.1.1. 2866.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_CLOAB

Accession

Primary (citable) accession number: Q97DY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)