Q97DY9 (ALR2_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase 2
EC=5.1.1.1

Gene names

Name:	alr2
Ordered Locus Names:	CA_C3331

Organism

Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) [Reference proteome] [HAMAP]

Taxonomic identifier

272562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium acetobutylicum

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Alanine racemase 2 HAMAP-Rule MF_01201		PRO_0000114510
Sites
	Active site	60	1	Proton acceptor; specific for D-alanine By similarity
	Active site	288	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	332	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	60	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97DY9 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 1A13F287C5C7CE43	FASTA	395	44,613
	10 20 30 40 50 60 MEKKTVYNIT NSIAISEEEA VNCNIPEVNK AEINLSQLKR NFDIIQGFLK PNTKFMAVLK 70 80 90 100 110 120 GDAYGHGIRP IAKELINLKC DVFGVVRLIE AFTLRKAGIK TPIMLLAPIS PSQAAWVIRY 130 140 150 160 170 180 NIIPMVDSEE IVEALDQSAS QNDTIVKLHV KINTGLNRYG VNPENAVKFI REIHEKYLHV 190 200 210 220 230 240 QVDGVYTHFQ DPDYNPDFTH KQIECFNNII FQLQKQKLRP RIIHAANSTG IIRYPEAHYD 250 260 270 280 290 300 MVRCGTLLFG LEHEQGQRNM PKGIKTLMEL KGRIMKVRTI RAGEAGGYGS TFVAKKDSKV 310 320 330 340 350 360 AIIAFGYGDG ISRGWKEVLV AGKRVPVVNY FMDGLMVDIS NIDETVKELD EAVIVGNQGD 370 380 390 ESITWLEACK SLGSYVDEQI QCITERVPKK YFYEK

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81263.1.
PIR	D97309.
RefSeq	NP_349923.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97DY9.
ModBase	Search...
Protein-protein interaction databases
STRING	272562.CA_C3331.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK81263; AAK81263; CA_C3331.
GeneID	1119513.
KEGG	cac:CA_C3331.
PATRIC	32041093. VBICloAce74127_3511.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031444.
KO	K01775.
OMA	AYHYGLE.
Enzyme and pathway databases
BioCyc	CACE272562:GJIH-3427-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_CLOAB

Accession

Primary (citable) accession number: Q97DY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program