Reviewed, UniProtKB/Swiss-Prot Q97DK4 (GUAC_CLOAB)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	CA_C3471

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	GMP reductase HAMAP MF_01511		PRO_0000093754
Regions
	Nucleotide binding	204 – 227	24	NADP By similarity
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97DK4-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: FA6105BC53D2D466	FASTA	327	36,280
	10 20 30 40 50 60 MESVFDYEDI QLIPAKCIVR SRSECDTSVI LGEHSFRLPV VPANMQTIID ENIALFLAQN 70 80 90 100 110 120 GYFYIMHRFE PEKRLSFIKN MKSKGLFASI SVGVKREEYD FIKQLAQENL SPEYITIDIA 130 140 150 160 170 180 HGHSNTVIEM IQHIKKYLPK SFVIAGNVGT PEAVRELEHA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCSKAA SKPIIADGGI RTPGDIAKSI RFGATMVMIG SLFAGHEESP 250 260 270 280 290 300 GETIEKDGKL YKEYFGSASE FQKGEKRNVE GKKMFVEYKG PLKDTLIEME QDLQSSISYA 310 320 GGKSLDAIRT VDYVIVKNSI FNGDRIY

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81399.1.
PIR	D97326.
RefSeq	NP_350059.1.
3D structure databases
SMR	Q97DK4. Positions 1-321.
ModBase	Search...
Genome annotation databases
GeneID	1119653.
GenomeReviews	Gene locus CA_C3471 in contig AE001437_GR.
KEGG	cac:CAC3471.
NMPDR	fig|272562.1.peg.3588.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
PhylomeDB	Q97DK4.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3471-MONOMER.
BRENDA	1.7.1.7. 2866.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_CLOAB

Accession

Primary (citable) accession number: Q97DK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)