Reviewed, UniProtKB/Swiss-Prot Q97DD9 (MURA2_CLOAB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Synonyms:	murZ
Ordered Locus Names:	CA_C3539

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity.
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000178864
Sites
	Active site	117	1	Proton donor By similarity
	Binding site	117	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97DD9-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 0D1346B6A8CCF629	FASTA	418	44,327
	10 20 30 40 50 60 MDKLVVNGGN PLFGSVEIGG AKNAAVAILP AAIMASEGIS VIDNIPDIQD IQRLERIITS 70 80 90 100 110 120 LGCKVKRVQN TVEIDSTNLT SVNADTEDGS KMRASYYLIG ALLGRFGKAK VELPGGCPIG 130 140 150 160 170 180 VRPIDQHIKG FEALGATVKI SHGTVEAQAD KLIGTNIYFD VVSVGATINL MLASVFAEGT 190 200 210 220 230 240 TVLENAAKEP HIVDVANFLN SMGANIKGAG TDVIRIAGVE KLKGCNYSVI PDQIEAATYM 250 260 270 280 290 300 IATAACGGCV TIKNVIPKHL ESISAKLIEM GADIKEGDDY VTIESHKNLK GVNIKTLPYP 310 320 330 340 350 360 GFPTDAQQPM STLLSISQGR SIVNESIWES RLKHVDELKK MGANIKVEGT VAIIDGVEKL 370 380 390 400 410 TGANVKATDL RAGAAMVIAA LAAEGVSEIS CIEHIDRGYP HIEDKFKELG ANIRREKI

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE001437 Genomic DNA. Translation: AAK81464.1.
PIR	E97334.
RefSeq	NP_350124.1.
3D structure databases
HSSP	HSSP built from PDB template 1EJD based on UniProtKB P33038.
ModBase	Search...
Genome annotation databases
GeneID	1119721.
GenomeReviews	Gene locus CA_C3539 in contig AE001437_GR.
KEGG	cac:CAC3539.
NMPDR	fig|272562.1.peg.3653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97DD9.
OMA	IDNIPDI.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3539-MON.
BRENDA	2.5.1.7. 2866.
Family and domain databases
HAMAP	MF_00111. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_CLOAB

Accession

Primary (citable) accession number: Q97DD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2001
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)