Reviewed, UniProtKB/Swiss-Prot Q97DC6 (LDH2_CLOAB)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	CA_C3552

Organism

Clostridium acetobutylicum [Complete proteome] [HAMAP]

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	L-lactate dehydrogenase 2 HAMAP MF_00488		PRO_0000168334
Regions
	Nucleotide binding	14 – 42	29	NAD By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	126	1	NAD or substrate By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97DC6-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 24B11585F6AA5247	FASTA	320	34,845
	10 20 30 40 50 60 MNFVKNKLVV VGAGMVGSAV LNSVLSLNLL SEVVIIDIND NKAKGEALDA SHTTSFAYSP 70 80 90 100 110 120 NVKVRAGNYE DCADAQIIVI TAGPSLKPDD KLDRLVLADT NVKVTDSIMK NICKYTKDAI 130 140 150 160 170 180 IIVVTNPVDI ATYYCQNNFD YPKNKIIGTG TLLDTARMRK IIGKKYNVDS KNVHGYVLGE 190 200 210 220 230 240 HGGSSFTSWS DVNIAGIPFN QLNDIFKDHY KVDKDEVDKE VRDSGIEVLK LKGYTSAGIA 250 260 270 280 290 300 MSVSRLVKAM LLNEQSILPV SSTLEGEYGI NDVALSIPCI ITSNGIEKKL EIPLSKDEVE 310 320 KLNKSADNLK SIIKGLNTNK

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81477.1.
PIR	B97336.
RefSeq	NP_350137.1.
3D structure databases
SMR	Q97DC6. Positions 5-317.
ModBase	Search...
Genome annotation databases
GeneID	1119734.
GenomeReviews	Gene locus CA_C3552 in contig AE001437_GR.
KEGG	cac:CAC3552.
NMPDR	fig|272562.1.peg.3666.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	CTEFGIG.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3552-MONOMER.
BRENDA	1.1.1.27. 2866.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_CLOAB

Accession

Primary (citable) accession number: Q97DC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)