Q97D80 (DAPA2_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase 2
Short name=DHDPS 2
EC=4.2.1.52

Gene names

Name:	dapA2
Ordered Locus Names:	CA_C3600

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Dihydrodipicolinate synthase 2 HAMAP MF_00418		PRO_0000103104
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	161	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97D80 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 527DBFE31C587C6E	FASTA	286	32,332
	10 20 30 40 50 60 MKIEGVLIPL ITPFKDGKVD LASYEKLIRH YSKKGVAGFM PLATTGETPT LSDYEYQSIL 70 80 90 100 110 120 EKTVECNELN LPIYVGFGGN NTEKMTKDIK MLDKYNIKGI LSVCPYYNRP DQRGIYEHFK 130 140 150 160 170 180 RISESTSLDI VLYNIPYRTG RNIENDTIRR LSELDNIVGV KDACGDFTQT TELLLNRPDN 190 200 210 220 230 240 FSILTGEDAF FYSTLMLGGD GGIMASAHLN TEKYVEVFNK SKQNDYKSAL EIWKQVANMI 250 260 270 280 PLLFEEPNPA PIKYCLSKTG VIASEALRLP LVPISENLKE KLNKFL

References

[1]

"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. Translation: AAK81523.1.
PIR	H97341.
RefSeq	NP_350183.1. NC_003030.1.
3D structure databases
ProteinModelPortal	Q97D80.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1119782.
GenomeReviews	Gene locus CA_C3600 in contig AE001437_GR.
KEGG	cac:CA_C3600.
NMPDR	fig|272562.1.peg.3712.
PATRIC	32041669. VBICloAce74127_3790.
Phylogenomic databases
HOGENOM	HBG358848.
OMA	PYRTGVR.
PhylomeDB	Q97D80.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	CACE272562:CAC3600-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA2_CLOAB

Accession

Primary (citable) accession number: Q97D80

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program