Q97CR6 (DP2L_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	TV0035
ORF Names:	TVG0041370

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	1088 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1088	1088	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152581

Sequences

	Sequence		Length	Mass (Da)
	Q97CR6 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 62B550A0A9BE7892	FASTA	1,088	122,362
	10 20 30 40 50 60 MTILHSTMLS EVLDLESYRK KISDEVRECF DLASQARELG MDVTDKVEIP LASDMAERIE 70 80 90 100 110 120 ALIGISGIAD QIRDLSKKMS REEVSLEMSR RVANIFKDNK KEALDKAIRV GLAILTEGIL 130 140 150 160 170 180 VAPLEGIADV YIGKNADGSD YVGISYAGPI RGAGGTAQAL SVLIGDVVRR ELGITRYIPT 190 200 210 220 230 240 EEEIERYIEE IESYDRIKHL QYLPTPDEIK LVVKNSPVCI DGEGSEEEEV SGHRDMARVK 250 260 270 280 290 300 TNRIRGGMCL VLCEGLVQKA RKILKYTSSM HLEEWSFLSS ISGKSDDKGS KKSDKFLKDI 310 320 330 340 350 360 VAGRPVFSHP SRPGGFRLRY GRSRVSGLAA ASLNPATMYI MGKFIAIGSQ IKVELPGKAA 370 380 390 400 410 420 AVTPCDTIDG PTVLLRNGDH VKINNIEKAK ELYDEVVEIT DAGEILIAYG DFLENNYNLP 430 440 450 460 470 480 TASFTKEWWM QYLPDGLDGN EVDQFRAVEL SRQYNIPLHP DFDYYWHDIS FEELEYLISA 490 500 510 520 530 540 VENGRLSDNS FLIPYSASEV LIKLGVQFKR SGNFLVLNQY YPLLVSLGYD VFEDKIKRVK 550 560 570 580 590 600 PYQRKSSVLE TVNYLSGITI KPRAPTRVGS RLGRPEKAGD RKMKPMVHSL FPVESYGEAR 610 620 630 640 650 660 RSILNANKKT DGTYKAEVFF YRCTSCGYES PSPTCPKCGS RSKPIGTKNS EIDLSVILRK 670 680 690 700 710 720 AEDRLGIKLD ELKEFKGVKK LMSKEKVAEP IEKGILRAIH GISVNKDGTC RFDMSDIPIT 730 740 750 760 770 780 HFRYKEIGIS KEKLSELGYE VKDVNEIFPQ DVIIPRKAAK YLFDVSKFID DLLVRYYGLP 790 800 810 820 830 840 PFYSLKSEED LVGHLVIGLA PHTSGGVVGR IIGFSDVNAF YAHPFFHAAK RRNCDGDEDS 850 860 870 880 890 900 VMLLMDGFLN FSARYLPSTT GGLMDAPLVL SVLINPDEID KEALNVDTLQ KYPLIFYEAT 910 920 930 940 950 960 ERHAAPSELE ETMMTMKVRI KKTATYRNSS YTFDTSDINK GVLVSSYKTL ATMDDKISEQ 970 980 990 1000 1010 1020 LGLARRIRAV DADDVAARVI STHFLPDMYG NFRRFFSQEF RCTKCNAKYR RIPLSGRCMK 1030 1040 1050 1060 1070 1080 CGSDSLTLTI HKGSVIKYLD ETLKIEKEYN IPKYLKERID NLARTIKETF PDEEKPDAAI KITGLDMY

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB59177.1.
RefSeq	NP_110554.1. NC_002689.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1441521.
GenomeReviews	Gene locus TV0035 in contig BA000011_GR.
NMPDR	fig|273116.1.peg.35.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_THEVO

Accession

Primary (citable) accession number: Q97CR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	October 1, 2001
Last modified:	May 31, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program