Q97CQ0 (VATA_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type ATP synthase alpha chain
EC=3.6.3.14

Alternative name(s):
V-ATPase subunit A

Cleaved into the following chain:

1. Tvo AtpA intein
   Alternative name(s):
   Tvo VMA intein

Gene names

Name:	atpA
Ordered Locus Names:	TV0051
ORF Names:	TVG0054274

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › Thermoplasma volcanium

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. HAMAP-Rule MF_00309
Catalytic activity	ATP + H2O + H+(In) = ADP + phosphate + H+(Out). HAMAP-Rule MF_00309
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation Potential. HAMAP-Rule MF_00309
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP-Rule MF_00309
Sequence similarities	Belongs to the ATPase alpha/beta chains family.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP
Cellular_component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP proton-transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	V-type ATP synthase alpha chain, 1st part Potential		PRO_0000002473
	Chain	236 – 421	186	Tvo AtpA intein Potential		PRO_0000002474
	Chain	422 – 776	355	V-type ATP synthase alpha chain, 2nd part Potential		PRO_0000002475

Sequences

	Sequence		Length	Mass (Da)
	Q97CQ0 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 50FBF72E1F5D5C50	FASTA	776	86,528
	10 20 30 40 50 60 MGKIVRISGP VVVAEDIENA KMYDVVKVGE MGLIGEIIRI EGNRSTIQVY EDTAGIRPDE 70 80 90 100 110 120 KVENTMRPLS VELGPGLLKS IYDGIQRPLD VIKETSGDFI ARGLNPPPLD RKKEWDFVPA 130 140 150 160 170 180 VKKNDIVYPG QVIGTVQETS LITHRIIVPD GVSGKIKSIY EGKRTVEDVV CTISTEHGDV 190 200 210 220 230 240 DVNLMTTWPV RKARRVVRKL PPEIPLVTGQ RVIDALFPVA KGGTAAVPGP FGSGKCVSGE 250 260 270 280 290 300 TPVYLADGKT IKIKDLYSSE RKKEDNIVEA GSGEEIIHLK DPIQIYSYVD GTIVRSRSRL 310 320 330 340 350 360 LYKGKSSYLV RIETIGGRSV SVTPVHKLFV LTEKGIEEVM ASNLKVGDMI AAVAESESEA 370 380 390 400 410 420 RDCGMSEECV MEAEVYTSLE ATFDRVKSIA YEKGDFDVYD LSVPEYGRNF IGGEGLLVLH 430 440 450 460 470 480 NTVIQHQLAK WSDANIVVYI GCGERGNEMT EILTTFPELK DPVSGQPLMD RTVLIANTSN 490 500 510 520 530 540 MPVAAREASI YTGITIAEYY RDMGYDVALM ADSTSRWAEA LREISGRLEE MPGEEGYPAY 550 560 570 580 590 600 LGRRISEFYE RSGRARLVSP EDRFGSITVI GAVSPPGGDI SEPVSQNTLR VTRVFWALDA 610 620 630 640 650 660 SLANRRHFPS INWLNSYSLY TEDLRHWYDE NVAKDWGSLR SQAMDILQRE SELQEVAQLV 670 680 690 700 710 720 GYDAMPEKEK SILDVARIIR EDFLQQSAFD EIDSYCSLRK QYLMLKAIME LNSYQSMAID 730 740 750 760 770 HGVTMDNLSS LPVREKLSRM KIVPEDQVES YYSSIIKEIH KEYTSFIGEK NAEANI

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB59193.1.
RefSeq	NP_110571.1. NC_002689.2.
3D structure databases
ProteinModelPortal	Q97CQ0.
SMR	Q97CQ0. Positions 422-741.
ModBase	Search...
Protein-protein interaction databases
STRING	273116.TVN0052.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB59193; BAB59193; BAB59193.
GeneID	1441538.
KEGG	tvo:TVN0052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1372.
KO	K02117.
OMA	VHNCGER.
ProtClustDB	CLSK2560425.
Family and domain databases
Gene3D	1.10.1140.10. 1 hit.
HAMAP	MF_00309. ATP_synth_A_arch. Divergent sequence.
InterPro	IPR020003. ATPase_a/bsu_AS. IPR004100. ATPase_a/bsu_N. IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR024034. ATPase_F1_bsu/V1_C. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006141. Intein_splice_site. IPR022878. V-ATPase_su_A/alpha. [Graphical view]
Pfam	PF00006. ATP-synt_ab. 2 hits. PF00306. ATP-synt_ab_C. 1 hit. PF02874. ATP-synt_ab_N. 1 hit. [Graphical view]
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF47917. ATPase_a/b_C. 1 hit. SSF50615. ATPase_a/b_N. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS00152. ATPASE_ALPHA_BETA. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VATA_THEVO

Accession

Primary (citable) accession number: Q97CQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program