ID   FTHS_THEVO              Reviewed;         536 AA.
AC   Q97CL3;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   27-MAR-2024, entry version 113.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=TV0088;
GN   ORFNames=TVG0090326;
OS   Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC
OS   15438 / GSS1).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1;
RX   PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA   Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y.,
RA   Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T.,
RA   Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT   "Archaeal adaptation to higher temperatures revealed by genomic sequence of
RT   Thermoplasma volcanium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; BA000011; BAB59230.1; -; Genomic_DNA.
DR   RefSeq; WP_010916345.1; NC_002689.2.
DR   AlphaFoldDB; Q97CL3; -.
DR   SMR; Q97CL3; -.
DR   STRING; 273116.gene:9380856; -.
DR   PaxDb; 273116-14324302; -.
DR   GeneID; 1441575; -.
DR   KEGG; tvo:TVG0090326; -.
DR   eggNOG; arCOG04541; Archaea.
DR   HOGENOM; CLU_003601_3_3_2; -.
DR   OrthoDB; 53075at2157; -.
DR   PhylomeDB; Q97CL3; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001017; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..536
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199416"
FT   BINDING         51..58
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   536 AA;  57913 MW;  5C560811AF6957D8 CRC64;
     MRDIEEILHE MGLSKDDYEL YGRYMAKLSL NLQEVNKKKA KLILVTAMTP TAAGEGKTTT
     TIGLGQAFKK LGKNVAIAIR EPSLGPCFGI KGGATGGGKS KVEPSDRINL LFTGDFPAIS
     AAHNLLSAMI NNHIYHGNEL NLDPKRITFP RTIDMNDRSL RSIVVGVGPR DMGAIANDNF
     VITPASEVMA ITGLSLNYKD LKEKLSRILA GFTVKNKPVF AADLKAEGSM AALLRDALKP
     NLVQTTEGVP AFVHTGPFGN IAHGTSSIVA DRIAMNLFDY VITEAGFGSD LGAEKFFNIV
     SRIGNLPINA VVLVATIRAL KLHGGSKKQG EDVEAVKEGS KNLLRHVANI RKFGVEPVVA
     LNKFPTDTEN EIMAIGEILD SHGIKWALSE VFEKGGEGGI ELANKVLSSI GDYQIKRTYE
     VREDLKTKIE KIAVNVYGAD GVIFEKKALN DLKKAEEIMN DPYVCMAKTQ YSFSDDASLL
     NDPHGFKVRV QSVNISSGAG FVVPILGEIM TMPGLPKRPA AENIDLAEDG EITGLF
//