ID   ENDA_THEVO              Reviewed;         292 AA.
AC   Q97BQ3;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   27-MAR-2024, entry version 107.
DE   RecName: Full=tRNA-splicing endonuclease {ECO:0000255|HAMAP-Rule:MF_01834};
DE            EC=4.6.1.16 {ECO:0000255|HAMAP-Rule:MF_01834};
DE   AltName: Full=tRNA-intron endonuclease {ECO:0000255|HAMAP-Rule:MF_01834};
GN   Name=endA {ECO:0000255|HAMAP-Rule:MF_01834}; OrderedLocusNames=TV0402;
GN   ORFNames=TVG0390826;
OS   Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC
OS   15438 / GSS1).
OC   Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC   Thermoplasmataceae; Thermoplasma.
OX   NCBI_TaxID=273116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1;
RX   PubMed=11121031; DOI=10.1073/pnas.97.26.14257;
RA   Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y.,
RA   Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T.,
RA   Yamamoto Y., Aramaki H., Makino K., Suzuki M.;
RT   "Archaeal adaptation to higher temperatures revealed by genomic sequence of
RT   Thermoplasma volcanium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000).
CC   -!- FUNCTION: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at
CC       the 5'- and 3'-splice sites to release the intron. The products are an
CC       intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and
CC       5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged
CC       loops of 3 bases are separated by a stem of 4 bp (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC         half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC         a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01834};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01834}.
CC   -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family. Archaeal
CC       long subfamily. {ECO:0000255|HAMAP-Rule:MF_01834}.
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DR   EMBL; BA000011; BAB59544.1; -; Genomic_DNA.
DR   RefSeq; WP_010916658.1; NC_002689.2.
DR   AlphaFoldDB; Q97BQ3; -.
DR   SMR; Q97BQ3; -.
DR   STRING; 273116.gene:9381180; -.
DR   PaxDb; 273116-14324617; -.
DR   GeneID; 1440916; -.
DR   KEGG; tvo:TVG0390826; -.
DR   eggNOG; arCOG01701; Archaea.
DR   HOGENOM; CLU_965070_0_0_2; -.
DR   OrthoDB; 46045at2157; -.
DR   PhylomeDB; Q97BQ3; -.
DR   Proteomes; UP000001017; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR   CDD; cd22363; tRNA-intron_lyase_C; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   Gene3D; 3.40.1350.150; -; 1.
DR   HAMAP; MF_01834; EndA_long; 1.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR   InterPro; IPR006676; tRNA_splic.
DR   InterPro; IPR023516; tRNA_splic_arch_long.
DR   NCBIfam; TIGR00324; endA; 1.
DR   PANTHER; PTHR21227; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1.
DR   PANTHER; PTHR21227:SF0; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1.
DR   Pfam; PF01974; tRNA_int_endo; 1.
DR   SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 1.
PE   3: Inferred from homology;
KW   Lyase; tRNA processing.
FT   CHAIN           1..292
FT                   /note="tRNA-splicing endonuclease"
FT                   /id="PRO_0000109491"
FT   ACT_SITE        231
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
FT   ACT_SITE        238
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
FT   ACT_SITE        267
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01834"
SQ   SEQUENCE   292 AA;  33923 MW;  CC93607C8E2966E1 CRC64;
     MSDGLCNGIS FNVSEGHGPN YIVGRYKLGF VQQGILFLDP YECLYLYFKG RITFEDSEES
     ALKKLFSITS VERYTAYTLL KNKGYRVHED NGTIFFRKST EIPRSVVVVR EYDEIDVGKF
     FTERPDFYFT VDEEGDATIY RIDEIDIKGT ENNKIGKCSV TDFGDRHFTK DDLPHWMGTK
     FHEYRMLTDF EANLIEGKEP KGMAEEVYRD LLSRGCIVKT GFKYGTNFRV YEGEKSEHAE
     YLVSVVENKL IWYYISRAVR VASSVRKKMI FTTIIDGKIK YIMISRSKDI VI
//