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Q97BQ3 (ENDA_THEVO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:TV0402
ORF Names:TVG0390826
OrganismThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]
Taxonomic identifier273116 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000109491

Sites

Active site2311 By similarity
Active site2381 By similarity
Active site2671 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97BQ3 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: CC93607C8E2966E1

FASTA29233,923
        10         20         30         40         50         60 
MSDGLCNGIS FNVSEGHGPN YIVGRYKLGF VQQGILFLDP YECLYLYFKG RITFEDSEES 

        70         80         90        100        110        120 
ALKKLFSITS VERYTAYTLL KNKGYRVHED NGTIFFRKST EIPRSVVVVR EYDEIDVGKF 

       130        140        150        160        170        180 
FTERPDFYFT VDEEGDATIY RIDEIDIKGT ENNKIGKCSV TDFGDRHFTK DDLPHWMGTK 

       190        200        210        220        230        240 
FHEYRMLTDF EANLIEGKEP KGMAEEVYRD LLSRGCIVKT GFKYGTNFRV YEGEKSEHAE 

       250        260        270        280        290 
YLVSVVENKL IWYYISRAVR VASSVRKKMI FTTIIDGKIK YIMISRSKDI VI 

« Hide

References

[1]"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium."
Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M.
Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000011 Genomic DNA. Translation: BAB59544.1.
RefSeqNP_110919.1. NC_002689.2.

3D structure databases

ProteinModelPortalQ97BQ3.
SMRQ97BQ3. Positions 1-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273116.TVN0400.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB59544; BAB59544; BAB59544.
GeneID1440916.
KEGGtvo:TVN0400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1676.
KOK01170.
OMAPYECLYL.
ProtClustDBPRK09300.

Enzyme and pathway databases

BioCycTVOL273116:GC31-413-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENDA_THEVO
AccessionPrimary (citable) accession number: Q97BQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families