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Q97BQ3

- ENDA_THEVO

UniProt

Q97BQ3 - ENDA_THEVO

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei231 – 2311UniRule annotation
    Active sitei238 – 2381UniRule annotation
    Active sitei267 – 2671UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciTVOL273116:GC31-413-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:TV0402
    ORF Names:TVG0390826
    OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
    Taxonomic identifieri273116 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001017: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292tRNA-splicing endonucleasePRO_0000109491Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273116.TVN0400.

    Structurei

    3D structure databases

    ProteinModelPortaliQ97BQ3.
    SMRiQ97BQ3. Positions 1-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    KOiK01170.
    OMAiPYECLYL.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q97BQ3-1 [UniParc]FASTAAdd to Basket

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    MSDGLCNGIS FNVSEGHGPN YIVGRYKLGF VQQGILFLDP YECLYLYFKG    50
    RITFEDSEES ALKKLFSITS VERYTAYTLL KNKGYRVHED NGTIFFRKST 100
    EIPRSVVVVR EYDEIDVGKF FTERPDFYFT VDEEGDATIY RIDEIDIKGT 150
    ENNKIGKCSV TDFGDRHFTK DDLPHWMGTK FHEYRMLTDF EANLIEGKEP 200
    KGMAEEVYRD LLSRGCIVKT GFKYGTNFRV YEGEKSEHAE YLVSVVENKL 250
    IWYYISRAVR VASSVRKKMI FTTIIDGKIK YIMISRSKDI VI 292
    Length:292
    Mass (Da):33,923
    Last modified:October 1, 2001 - v1
    Checksum:iCC93607C8E2966E1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB59544.1.
    RefSeqiNP_110919.1. NC_002689.2.
    WP_010916658.1. NC_002689.2.

    Genome annotation databases

    EnsemblBacteriaiBAB59544; BAB59544; BAB59544.
    GeneIDi1440916.
    KEGGitvo:TVN0400.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB59544.1 .
    RefSeqi NP_110919.1. NC_002689.2.
    WP_010916658.1. NC_002689.2.

    3D structure databases

    ProteinModelPortali Q97BQ3.
    SMRi Q97BQ3. Positions 1-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273116.TVN0400.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB59544 ; BAB59544 ; BAB59544 .
    GeneIDi 1440916.
    KEGGi tvo:TVN0400.

    Phylogenomic databases

    eggNOGi COG1676.
    KOi K01170.
    OMAi PYECLYL.

    Enzyme and pathway databases

    BioCyci TVOL273116:GC31-413-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

    Entry informationi

    Entry nameiENDA_THEVO
    AccessioniPrimary (citable) accession number: Q97BQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3