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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei231UniRule annotation1
Active sitei238UniRule annotation1
Active sitei267UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciTVOL273116:G1G3C-423-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:TV0402
ORF Names:TVG0390826
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001017 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094911 – 292tRNA-splicing endonucleaseAdd BLAST292

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273116.TVN0400

Structurei

3D structure databases

ProteinModelPortaliQ97BQ3
SMRiQ97BQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
KOiK01170
OMAiPYECLYL
OrthoDBiPOG093Z0DMC

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q97BQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGLCNGIS FNVSEGHGPN YIVGRYKLGF VQQGILFLDP YECLYLYFKG
60 70 80 90 100
RITFEDSEES ALKKLFSITS VERYTAYTLL KNKGYRVHED NGTIFFRKST
110 120 130 140 150
EIPRSVVVVR EYDEIDVGKF FTERPDFYFT VDEEGDATIY RIDEIDIKGT
160 170 180 190 200
ENNKIGKCSV TDFGDRHFTK DDLPHWMGTK FHEYRMLTDF EANLIEGKEP
210 220 230 240 250
KGMAEEVYRD LLSRGCIVKT GFKYGTNFRV YEGEKSEHAE YLVSVVENKL
260 270 280 290
IWYYISRAVR VASSVRKKMI FTTIIDGKIK YIMISRSKDI VI
Length:292
Mass (Da):33,923
Last modified:October 1, 2001 - v1
Checksum:iCC93607C8E2966E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA Translation: BAB59544.1
RefSeqiWP_010916658.1, NC_002689.2

Genome annotation databases

EnsemblBacteriaiBAB59544; BAB59544; BAB59544
GeneIDi1440916
KEGGitvo:TVG0390826

Entry informationi

Entry nameiENDA_THEVO
AccessioniPrimary (citable) accession number: Q97BQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2001
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health