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Q97BQ3

- ENDA_THEVO

UniProt

Q97BQ3 - ENDA_THEVO

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei231 – 2311UniRule annotation
Active sitei238 – 2381UniRule annotation
Active sitei267 – 2671UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciTVOL273116:GC31-413-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:TV0402
ORF Names:TVG0390826
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001017: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292tRNA-splicing endonucleasePRO_0000109491Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273116.TVN0400.

Structurei

3D structure databases

ProteinModelPortaliQ97BQ3.
SMRiQ97BQ3. Positions 1-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
KOiK01170.
OMAiPYECLYL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q97BQ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDGLCNGIS FNVSEGHGPN YIVGRYKLGF VQQGILFLDP YECLYLYFKG
60 70 80 90 100
RITFEDSEES ALKKLFSITS VERYTAYTLL KNKGYRVHED NGTIFFRKST
110 120 130 140 150
EIPRSVVVVR EYDEIDVGKF FTERPDFYFT VDEEGDATIY RIDEIDIKGT
160 170 180 190 200
ENNKIGKCSV TDFGDRHFTK DDLPHWMGTK FHEYRMLTDF EANLIEGKEP
210 220 230 240 250
KGMAEEVYRD LLSRGCIVKT GFKYGTNFRV YEGEKSEHAE YLVSVVENKL
260 270 280 290
IWYYISRAVR VASSVRKKMI FTTIIDGKIK YIMISRSKDI VI
Length:292
Mass (Da):33,923
Last modified:October 1, 2001 - v1
Checksum:iCC93607C8E2966E1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000011 Genomic DNA. Translation: BAB59544.1.
RefSeqiNP_110919.1. NC_002689.2.
WP_010916658.1. NC_002689.2.

Genome annotation databases

EnsemblBacteriaiBAB59544; BAB59544; BAB59544.
GeneIDi1440916.
KEGGitvo:TVN0400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000011 Genomic DNA. Translation: BAB59544.1 .
RefSeqi NP_110919.1. NC_002689.2.
WP_010916658.1. NC_002689.2.

3D structure databases

ProteinModelPortali Q97BQ3.
SMRi Q97BQ3. Positions 1-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273116.TVN0400.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB59544 ; BAB59544 ; BAB59544 .
GeneIDi 1440916.
KEGGi tvo:TVN0400.

Phylogenomic databases

eggNOGi COG1676.
KOi K01170.
OMAi PYECLYL.

Enzyme and pathway databases

BioCyci TVOL273116:GC31-413-MONOMER.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Entry informationi

Entry nameiENDA_THEVO
AccessioniPrimary (citable) accession number: Q97BQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2001
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3