Reviewed, UniProtKB/Swiss-Prot Q97BJ8 (G3P_THEVO)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	TV0457
ORF Names:	TVG0444310

Organism

Thermoplasma volcanium [Complete proteome] [HAMAP]

Taxonomic identifier

50339 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145738
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	138 – 140	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	192 – 193	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	139	1	Nucleophile By similarity
	Binding site	109	1	NAD; via amide nitrogen By similarity
	Binding site	167	1	NAD By similarity
	Binding site	169	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	299	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97BJ8-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: C9BD53DFD4722F34	FASTA	338	37,374
	10 20 30 40 50 60 MIRVGINGYG TIGRRVANAV SSQDDMIVVG IVKTKPDYIS EVASRRFKIF VPDSSYMKAF 70 80 90 100 110 120 QDAGIKVEGT LDNLLDDAEI IVDATPEGMG EKNKPLYIKK KAKAIFEGGE EPDVAETSFN 130 140 150 160 170 180 AYSNYNDAIG KSYVRVVSCN TTGLARTLYP IQQAFGVKHV EATLIRRATD QNDSSKGPIN 190 200 210 220 230 240 AVEPSLKIPS HHAPDLKTVM GNIDVNTVAI KVPTTLMHVH VVQVDTEKNA SNDGVLEAWN 250 260 270 280 290 300 NYRRIIHVKK DDGIKSTAQI MDLAREFGRD RSDLYEIAIW EGSVSAKANR ISYIQAVHQE 310 320 330 SDVIPENVDA IRAMFNLADK EKSIEKTDKS LGIEKRVY

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / IFO 15438 / JCM 9571 / GSS1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000011 Genomic DNA. Translation: BAB59599.1.
RefSeq	NP_110977.1.
3D structure databases
HSSP	HSSP built from PDB template 1CF2 based on UniProtKB P10618.
ModBase	Search...
Genome annotation databases
GeneID	1440974.
GenomeReviews	Gene locus TV0457 in contig BA000011_GR.
KEGG	tvo:TVN0458.
NMPDR	fig|273116.1.peg.458.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q97BJ8.
OMA	Q97BJ8. AIFQGGE.
Enzyme and pathway databases
BRENDA	1.2.1.59. 256346.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_THEVO

Accession

Primary (citable) accession number: Q97BJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	October 1, 2001
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)