Q97BD7 (RIFK_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	TV0519
ORF Names:	TVG0510159

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Riboflavin kinase HAMAP MF_01285		PRO_0000322108
Regions
	Nucleotide binding	102 – 107	6	CDP By similarity
	Nucleotide binding	200 – 203	4	CDP By similarity
	Region	1 – 92	92	H-T-H motif-like HAMAP MF_01285
	Region	93 – 220	128	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	131	1	Magnesium By similarity
	Metal binding	133	1	Magnesium By similarity
	Binding site	188	1	FMN By similarity
	Binding site	195	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97BD7 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 3ED2D5F033A08FE3	FASTA	220	24,808
	10 20 30 40 50 60 MDTSDQYYRA IKKIKEASDS SNKAYLTSSK LANMLGLSQQ SASRIIIDLE KLGYITRTVS 70 80 90 100 110 120 KRGQLLTITE KGLDLLYTEF AELSRILSIK SNIVMTGIVV PGMGEGKYYI SRKQYIIQFQ 130 140 150 160 170 180 EKLGIIPYLG TLNIKVDPSS IPELRKLRGF TGIHIEGFRT EDRTFGSVKA FRCKTNGVPS 190 200 210 220 FLIMPERTVY TDVVEIISDK YLRNELNLKD GDEVTIEVTA

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB59661.1.
RefSeq	NP_111038.1. NC_002689.2.
3D structure databases
ProteinModelPortal	Q97BD7.
SMR	Q97BD7. Positions 5-206.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1441035.
GenomeReviews	Gene locus TV0519 in contig BA000011_GR.
KEGG	tvo:TVN0519.
NMPDR	fig|273116.1.peg.519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	PRK14165.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_THEVO

Accession

Primary (citable) accession number: Q97BD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2001
Last modified:	November 16, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program