Q97BD5 (PURL_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	TV0521
ORF Names:	TVG0510425

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100528
Regions
	Nucleotide binding	120 – 131	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q97BD5 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: EBDDF8DC566FAE6A	FASTA	759	84,194
	10 20 30 40 50 60 MIYRHIKIRD MNDDDLINMS KRHGLSLSLD EMKAVRDYFI KEGRDPIDAE IHAIAQSWSE 70 80 90 100 110 120 HCSYKSSKFY LKKYLTNLKT DYTILAMEDD AGVVEFDKDY AYVLKMESHN HPSAVEPYGG 130 140 150 160 170 180 AATGIGGIVR DVVCMGAQPI ALVDSLFMGD VASQKYDALL SPRYIFQGVV GGIRDYGNRI 190 200 210 220 230 240 GVPNVAGSVY FDQSYNSNPL VNAGCIGIVR KDRIVRSKSY KAGDKLLLVG GKTGRDGIHG 250 260 270 280 290 300 VNFASATLTR ISKSSRNAIQ LGNPIVEHPM MRAVLEANEL GIIKAMKDLG GGGLSSAATE 310 320 330 340 350 360 MVFAGGFGAE ISLDDIKLKD TDMSGWEIWI SESQERMLVE VLPEDVEKMK EIAEKWSLDF 370 380 390 400 410 420 SILGTVVEGK KITVRYKNKK IIDMDIEFLD KAPVYQRPFE RKNIEKKVSI PSEPEDLNDF 430 440 450 460 470 480 ALNFVSRLNN SARFNVVRQY DHTVRGSTIV GPFSGRPNLE THSDATVIKP LEDSMRGLLI 490 500 510 520 530 540 TSGSRPNFVS IDPYNGTLET LSEAVRNIVS TGGKPNSVVD ALNFGNPERQ DIMGQFVESV 550 560 570 580 590 600 RAIGDFCRKF SLPVVAGNVS FYNEFRNKDI MPTPTIMMVG IIDDVTKART TYFKKNKSQI 610 620 630 640 650 660 YLVGTPCDNL SGSEYARMNN IFDGFLPAPN LSELQLEIEK IGKFSPYILS AHDVSDGGLF 670 680 690 700 710 720 MALAEMSFGS GIGFNIDLGN VSASRSSVKL FSECGNQIVL EIDPIHEEEF TAEFKDLKIV 730 740 750 RIGETGGDRI IIDEYGMNLV DLPVQDLRER WEHGLDAYI

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB59663.1.
RefSeq	NP_111040.1. NC_002689.2.
3D structure databases
ProteinModelPortal	Q97BD5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1441037.
GenomeReviews	Gene locus TV0521 in contig BA000011_GR.
KEGG	tvo:TVN0521.
NMPDR	fig|273116.1.peg.521.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q97BD5.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_THEVO

Accession

Primary (citable) accession number: Q97BD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program