Q97B26 (HEM3_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	TV0633
ORF Names:	TVG0625731

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › Thermoplasma volcanium

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethane group By similarity. HAMAP-Rule MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Probable porphobilinogen deaminase HAMAP-Rule MF_00260		PRO_0000143033
Amino acid modifications
	Modified residue	233	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97B26 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 01067904DF95820D	FASTA	297	32,892
	10 20 30 40 50 60 MKIKIGTRPS NLAVAQANMV ASSLEAIGID TEIVKHRSAG DIDTKNPIYS IGKTGVFVQD 70 80 90 100 110 120 LNNMILRGEI DVAVHSAKDI PSEIENRLTI AATLKRGSFN DALVSKYDLR SLPISAKVGT 130 140 150 160 170 180 SSIRRIFQLK YARQDLKFEN IRGNIETRIA KMSELGLDAI VMAEAAIQRL GLNVEYSKLD 190 200 210 220 230 240 ENKFVPAPNQ GIIAVVSKKE GTVRKILEEI NDENTFEEMQ IERSVVQQLK LGCSTPVGIL 250 260 270 280 290 SRPSGKGHKI IAQFFSMQGD DVSVFEQYLN DLSDVDSLVS YIRENIPREY GYIASEE

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB59775.1.
RefSeq	NP_111153.1. NC_002689.2.
3D structure databases
ProteinModelPortal	Q97B26.
ModBase	Search...
Protein-protein interaction databases
STRING	273116.TVN0634.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB59775; BAB59775; BAB59775.
GeneID	1441740.
KEGG	tvo:TVN0634.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0181.
KO	K01749.
OMA	QPLRGNV.
ProtClustDB	PRK00072.
Enzyme and pathway databases
UniPathway	UPA00251; UER00319.
Family and domain databases
Gene3D	3.30.160.40. 1 hit.
HAMAP	MF_00260. Porphobil_deam.
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
PANTHER	PTHR11557. PTHR11557. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. hemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_THEVO

Accession

Primary (citable) accession number: Q97B26

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program