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Q97AN8 (SYL_THEVO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:TV0772
ORF Names:TVG0775903
OrganismThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]
Taxonomic identifier273116 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length910 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 910910Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152149

Regions

Motif50 – 6011"HIGH" region HAMAP-Rule MF_00049
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q97AN8 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: C21870CE07188ED3

FASTA910104,994
        10         20         30         40         50         60 
MDYSRCCGST NSRVIYMNID EKWQNAWERD HVFEPKIDER KKFMITVPWP YTNGSLHVGH 

        70         80         90        100        110        120 
GRTYTLGDII ARYKRSRNYN VLFPMGFHQS GTPILAFSER IRAGDASTIA LYRSYLSEYG 

       130        140        150        160        170        180 
EKDIDGWLEK FKDPRNIADY FSNAIINDFK HLGYSIDWTR KFTSADEFYQ NVVKWQFHKL 

       190        200        210        220        230        240 
NEKGLIKQDK YPILYSIDDD NAVGEDDIKD GDTDKVSVEE YTAVFFESNS YSLIAASLRP 

       250        260        270        280        290        300 
ETLFGVTNIW INPTGEYVKI KIGDKIAVVS KEAVDKLKYQ RNDVSVIGPI SAESIQRKKF 

       310        320        330        340        350        360 
TTPFGKEVPV YKADFVDTDN GTGVVYSVPS HSVYDFVYYR RKKSGQTPVV IEAPLKMPEV 

       370        380        390        400        410        420 
EIKFDLNSKE GLDEATKELY KSEFYYGKLV NSGEYTGLTV RDAREKIKKD LIGSGKAIIF 

       430        440        450        460        470        480 
YETSRKAVTR GGSKVIVAVL PDQWFIDYSA DWLKKLSHDM LNRMMIYPEM YRNVMNDAID 

       490        500        510        520        530        540 
WLKERPCARR RGLGTKLPFD DRWVIESLSD STIYPAVYTT SIQMRKLYEN GKLDENAIER 

       550        560        570        580        590        600 
IFDGGEVQND EERTARNEFS YWYPVDIRLT AVPHISNHLS FYVMNHAAIF PPEKWPSGLI 

       610        620        630        640        650        660 
ISGLVVSNGA KISKSKGNVV SLLEITKKYS ADIYRLYVAV QADVSSTMDW NENDLSNIVR 

       670        680        690        700        710        720 
RFNEFKTIMD SFKPDTSELN FEETWFVSRF AERLKQFMDQ MDGFQIRDAY INIFYGTLND 

       730        740        750        760        770        780 
LKYAVNRGAS QNRSLASIIA DWLRALMPVI SHHAEEYWHR YVSNTYVSIN PFDDNFAEKY 

       790        800        810        820        830        840 
ERLAKVYGLS TSEFYQVMDY VEHIIQDINN IISVTGIEPK SVEITVANED VIKASREFLS 

       850        860        870        880        890        900 
NSVSERSKRY LQYLAKRRKD IVVYPFNEID ILRRNSSYIS RQVKADVSIN TGDIINGKIA 

       910 
VPGKPVIHIT 

« Hide

References

[1]"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium."
Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M.
Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000011 Genomic DNA. Translation: BAB59914.1.
RefSeqNP_111280.1. NC_002689.2.

3D structure databases

ProteinModelPortalQ97AN8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273116.TVN0761.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB59914; BAB59914; BAB59914.
GeneID1441867.
KEGGtvo:TVN0761.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAQNDEERT.

Enzyme and pathway databases

BioCycTVOL273116:GC31-786-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEVO
AccessionPrimary (citable) accession number: Q97AN8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2001
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries