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Q97AG7

- SYI_THEVO

UniProt

Q97AG7 - SYI_THEVO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei594 – 5941ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTVOL273116:GC31-856-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TV0843
    ORF Names:TVG0867938
    OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
    Taxonomic identifieri273116 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001017: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10281028Isoleucine--tRNA ligasePRO_0000098596Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273116.TVN0829.

    Structurei

    3D structure databases

    ProteinModelPortaliQ97AG7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi51 – 6111"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiRVEHMVE.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q97AG7-1 [UniParc]FASTAAdd to Basket

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    MQAQRYRLIE QGNTIMDIDN EILRYWNDHA INEKIFKKEG TKKFVFLEGP     50
    PTANGRPHIG HAMTRTIKDI VLRYNTMTGH KIYRRYGGWD CHGLPVELEA 100
    EKYFGFKTKS DIINYGVEKF NSYCRDSVFR YIDEWKTVDQ IIGFSIDHSG 150
    DYITLRNEYI ESEWYVLKYI YENGLLYKDY TVVPYCPRCE TSLSSHEVAQ 200
    GYKDVKDPSV YVRFKEKGSE NTYFVAWTTT PWTLPSNEFL VVNPDIEYSL 250
    IEYNNAKYYV ASSRAQYIFK DFKVLKKMKG FELAGKGYEQ LLPFLDPPAG 300
    ALRVVTGDFV TDIDGSGIVH AAPAFGSDDY QIGKRENVPI LNPVDKNGRF 350
    SDERLPWYGK KVREANEDII VYLKNNGLLI KSEKYEHSYP FCYRCDTPLL 400
    YYPLDAWFIR VSSVRDKLVE NNEKIRWKPD YLKHGRFGNF LDEAKDWNLS 450
    RDRFWGTPLP VWRCRNNHVR FIGSRKEIEE MGATVPQDLH RPYIDEVKFV 500
    CPDCGEEMRR EPYVIDTWFD SGSATYAAMH YPFEDNFDPS SDLPVSFITE 550
    AIDQTRGWFY VLHVISTIMF NKNAYDSALS ISFILDEQGR KMSKSKGNSV 600
    FALDYLKQVA PDSLRLFFLY GAPWKSKNLD RKIIDEVSRR VISTLLNVYS 650
    FFAYNANIDG FEFKGILEAK DTLDKWLISK INTFIIESRR AYDDLDFHEV 700
    VRLSMDFVDN LSNFYLRLSR RRFWAEDVTE DKLAAYSTLY TAIMTCSKVL 750
    APIVPFVSDY LYLSLHGPYE SIHLDSFPEP DTSKIDHDLE KRMDQAYSVI 800
    ETVRRIRQEI NIKGRQPVKE ILLSGNIDPE IIPVVSQEVN AKEIRIVSSE 850
    QRPLKYTVDL KMETAAPILR SSVNSVREAL KSIDGKAAFD AVQNGGKLRV 900
    LDHELTGEML NISTIPDPDY GYSRDEKNGI DVFVNKRIDR NEYLEGLARE 950
    IVRRIQLMRK EMNLDYTDRI NVWIDPVGDF SDAIDKFESY IKAETQCDSL 1000
    NVGHTDDLRK WEIGDETIGI RIEKVVPK 1028
    Length:1,028
    Mass (Da):119,494
    Last modified:October 1, 2001 - v1
    Checksum:iABBBCD972B46641F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB59985.1.
    RefSeqiNP_111348.1. NC_002689.2.

    Genome annotation databases

    EnsemblBacteriaiBAB59985; BAB59985; BAB59985.
    GeneIDi1441935.
    KEGGitvo:TVN0829.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB59985.1 .
    RefSeqi NP_111348.1. NC_002689.2.

    3D structure databases

    ProteinModelPortali Q97AG7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273116.TVN0829.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB59985 ; BAB59985 ; BAB59985 .
    GeneIDi 1441935.
    KEGGi tvo:TVN0829.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi RVEHMVE.

    Enzyme and pathway databases

    BioCyci TVOL273116:GC31-856-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

    Entry informationi

    Entry nameiSYI_THEVO
    AccessioniPrimary (citable) accession number: Q97AG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3