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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTVOL273116:GC31-856-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TV0843
ORF Names:TVG0867938
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001017 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Isoleucine--tRNA ligasePRO_0000098596Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273116.TVN0829.

Structurei

3D structure databases

ProteinModelPortaliQ97AG7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiWGTPLNI.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q97AG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAQRYRLIE QGNTIMDIDN EILRYWNDHA INEKIFKKEG TKKFVFLEGP
60 70 80 90 100
PTANGRPHIG HAMTRTIKDI VLRYNTMTGH KIYRRYGGWD CHGLPVELEA
110 120 130 140 150
EKYFGFKTKS DIINYGVEKF NSYCRDSVFR YIDEWKTVDQ IIGFSIDHSG
160 170 180 190 200
DYITLRNEYI ESEWYVLKYI YENGLLYKDY TVVPYCPRCE TSLSSHEVAQ
210 220 230 240 250
GYKDVKDPSV YVRFKEKGSE NTYFVAWTTT PWTLPSNEFL VVNPDIEYSL
260 270 280 290 300
IEYNNAKYYV ASSRAQYIFK DFKVLKKMKG FELAGKGYEQ LLPFLDPPAG
310 320 330 340 350
ALRVVTGDFV TDIDGSGIVH AAPAFGSDDY QIGKRENVPI LNPVDKNGRF
360 370 380 390 400
SDERLPWYGK KVREANEDII VYLKNNGLLI KSEKYEHSYP FCYRCDTPLL
410 420 430 440 450
YYPLDAWFIR VSSVRDKLVE NNEKIRWKPD YLKHGRFGNF LDEAKDWNLS
460 470 480 490 500
RDRFWGTPLP VWRCRNNHVR FIGSRKEIEE MGATVPQDLH RPYIDEVKFV
510 520 530 540 550
CPDCGEEMRR EPYVIDTWFD SGSATYAAMH YPFEDNFDPS SDLPVSFITE
560 570 580 590 600
AIDQTRGWFY VLHVISTIMF NKNAYDSALS ISFILDEQGR KMSKSKGNSV
610 620 630 640 650
FALDYLKQVA PDSLRLFFLY GAPWKSKNLD RKIIDEVSRR VISTLLNVYS
660 670 680 690 700
FFAYNANIDG FEFKGILEAK DTLDKWLISK INTFIIESRR AYDDLDFHEV
710 720 730 740 750
VRLSMDFVDN LSNFYLRLSR RRFWAEDVTE DKLAAYSTLY TAIMTCSKVL
760 770 780 790 800
APIVPFVSDY LYLSLHGPYE SIHLDSFPEP DTSKIDHDLE KRMDQAYSVI
810 820 830 840 850
ETVRRIRQEI NIKGRQPVKE ILLSGNIDPE IIPVVSQEVN AKEIRIVSSE
860 870 880 890 900
QRPLKYTVDL KMETAAPILR SSVNSVREAL KSIDGKAAFD AVQNGGKLRV
910 920 930 940 950
LDHELTGEML NISTIPDPDY GYSRDEKNGI DVFVNKRIDR NEYLEGLARE
960 970 980 990 1000
IVRRIQLMRK EMNLDYTDRI NVWIDPVGDF SDAIDKFESY IKAETQCDSL
1010 1020
NVGHTDDLRK WEIGDETIGI RIEKVVPK
Length:1,028
Mass (Da):119,494
Last modified:October 1, 2001 - v1
Checksum:iABBBCD972B46641F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB59985.1.
RefSeqiNP_111348.1. NC_002689.2.
WP_010917087.1. NC_002689.2.

Genome annotation databases

EnsemblBacteriaiBAB59985; BAB59985; BAB59985.
GeneIDi1441935.
KEGGitvo:TVN0829.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB59985.1.
RefSeqiNP_111348.1. NC_002689.2.
WP_010917087.1. NC_002689.2.

3D structure databases

ProteinModelPortaliQ97AG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273116.TVN0829.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB59985; BAB59985; BAB59985.
GeneIDi1441935.
KEGGitvo:TVN0829.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiWGTPLNI.

Enzyme and pathway databases

BioCyciTVOL273116:GC31-856-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Entry informationi

Entry nameiSYI_THEVO
AccessioniPrimary (citable) accession number: Q97AG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2001
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.