Q97A76 (PIP_THEVO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline iminopeptidase
Short name=PIP
EC=3.4.11.5

Alternative name(s):
Prolyl aminopeptidase
Short name=PAP
Tricorn protease-interacting factor F1

Gene names

Name:	pip
Ordered Locus Names:	TV0934
ORF Names:	TVG0960913

Organism

Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]

Taxonomic identifier

273116 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. In conjunction with the three factors F1, F2 and F3, Tricorn degrades oligopeptides in a sequential manner, yielding free amino acids By similarity.
Catalytic activity	Release of N-terminal proline from a peptide.
Subunit structure	Part of the tricorn proteolytic complex By similarity.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Proline iminopeptidase		PRO_0000080852
Sites
	Active site	107	1	Nucleophile By similarity
	Active site	246	1	By similarity
	Active site	273	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q97A76 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 821B4C15E3EDD860	FASTA	295	33,830
	10 20 30 40 50 60 MRKLSRCEDG YVKIQGIYIY YKVCKAENEK AKLMTLHGGP GMSHDYLLSL TDLAEKGITV 70 80 90 100 110 120 LFYDQFGCGR SEEPEKEKFT IDYGVEEAEA VKKNIFGDDK VFLMGSSYGG ALALAYAVKY 130 140 150 160 170 180 QAHLKGLIIS GGLSSVPLTV KEMQRLIDEL PEKYRNAIRK YGEVGDYQNP EYQEAVNYFY 190 200 210 220 230 240 HQHLLRSEDW PPEVLKSLEY AEERNVYRTM NGPNEFTITG TIRDWDITDK IGIISVPTLI 250 260 270 280 290 TVGEFDEVTQ NVAEVIHSKI DNSQLIVFKA CSHLTMWEDR DEYNRILLQF IEKNI

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB60076.1.
RefSeq	NP_111429.1. NC_002689.2.
3D structure databases
ProteinModelPortal	Q97A76.
SMR	Q97A76. Positions 7-295.
ModBase	Search...
Protein family/group databases
MEROPS	S33.005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1442016.
GenomeReviews	Gene locus TV0934 in contig BA000011_GR.
NMPDR	fig|273116.1.peg.910.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG515521.
OMA	MENITAA.
ProtClustDB	CLSK374918.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR005945. Pept_S33_TRI_F1. IPR002410. Peptidase_S33. [Graphical view]
PIRSF	PIRSF005539. Pept_S33_TRI_F1. 1 hit.
PRINTS	PR00111. ABHYDROLASE. PR00793. PROAMNOPTASE.
TIGRFAMs	TIGR01250. Pro_imino_pep_2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIP_THEVO

Accession

Primary (citable) accession number: Q97A76

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2001
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program