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Protein

Enolase

Gene

eno

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (TVG1076108)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146SubstrateUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Active sitei197Proton donorUniRule annotation1
Metal bindingi233MagnesiumUniRule annotation1
Metal bindingi274MagnesiumUniRule annotation1
Binding sitei274SubstrateUniRule annotation1
Metal bindingi301MagnesiumUniRule annotation1
Binding sitei301SubstrateUniRule annotation1
Active sitei326Proton acceptorUniRule annotation1
Binding sitei326Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei377SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciTVOL273116:G1G3C-1073-MONOMER
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:TV1011
ORF Names:TVG1033459
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001017 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340381 – 401EnolaseAdd BLAST401

Interactioni

Protein-protein interaction databases

STRINGi273116.TVN0981

Structurei

3D structure databases

ProteinModelPortaliQ979Z9
SMRiQ979Z9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni353 – 356Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01169 Archaea
COG0148 LUCA
KOiK01689
OMAiQEFLVVP
OrthoDBiPOG093Z03D9

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q979Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELPIEDVRV RKVLDSRGNF TVEADVYIPG GFGRTSAPAG ASTGETEVIA
60 70 80 90 100
FSKKGIDESI KFFETNVRRS IIGFNALDQK GFDALITDLD GSGNFSNLGG
110 120 130 140 150
NLSTALSMSV AKAVSAHLGI PLYRYVGGIN HSMPRPIGNV IGGGKHARNG
160 170 180 190 200
TSIQEFLVSA QGKTFMESAY VNVLVHRKIG DILSEKMKDI SIGVGDERAW
210 220 230 240 250
SVNISDEEAF EVLNQAVNEV SSETKVKIYT GLDFAADSLY ENGKYVYKHT
260 270 280 290 300
VRSRDEQIDY AISINKDFGV YYIEDPMFDT DFEGFAEITK KIGDKAMIVG
310 320 330 340 350
DDLYTTNPDR IRKGIELGST NAVLIKVNQI GTLTKAQEAA SLASSAGLKN
360 370 380 390 400
VVSHRSGETT DDFLAHLSVA FSSTFVKTGT IGGERIAKLN ELMRIEECLT

S
Length:401
Mass (Da):43,517
Last modified:October 1, 2001 - v1
Checksum:iC14F77CA1F87016C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA Translation: BAB60153.1
RefSeqiWP_010917239.1, NC_002689.2

Genome annotation databases

EnsemblBacteriaiBAB60153; BAB60153; BAB60153
GeneIDi1441121
KEGGitvo:TVG1033459

Similar proteinsi

Entry informationi

Entry nameiENO_THEVO
AccessioniPrimary (citable) accession number: Q979Z9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: October 1, 2001
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health