Reviewed, UniProtKB/Swiss-Prot Q979P8 (GUAAA_THEVO)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	TV1112
ORF Names:	TVG1144148

Organism

Thermoplasma volcanium [Complete proteome] [HAMAP]

Taxonomic identifier

50339 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_01510
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140236
Regions
	Domain	4 – 194	191	Glutamine amidotransferase type-1
Sites
	Active site	81	1	Nucleophile By similarity
	Active site	168	1	By similarity
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q979P8-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 83F5B81190425182	FASTA	202	22,881
	10 20 30 40 50 60 MIMKIYVVDN GGQWTHREWR VLRELGVETE IVPNDIESRK LDGLDGLVLS GGAPNIDEEM 70 80 90 100 110 120 DKLGSIGEYI EDHDYPVFGI CVGAQFIALH YGAQVVKAKH PEFGKTLVKI SEPANVLAGL 130 140 150 160 170 180 PENIIAWENH NDEIKNLTSD FVLTASSETC EVQAFYHKHK PLYAVQFHPE VEHTQFGREI 190 200 FKNFIAICQQ HKEKLINNVN NK

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / IFO 15438 / JCM 9571 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB60254.1.
RefSeq	NP_111607.1.
3D structure databases
SMR	Q979P8. Positions 2-193.
ModBase	Search...
Genome annotation databases
GeneID	1441228.
GenomeReviews	Gene locus TV1112 in contig BA000011_GR.
KEGG	tvo:TVN1088.
NMPDR	fig|273116.1.peg.1088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG292341.
OMA	GQYVHRI.
Enzyme and pathway databases
BRENDA	6.3.5.2. 256346.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR006220. Anth_synthII. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_THEVO

Accession

Primary (citable) accession number: Q979P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)