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Q978W6

- KAE1B_THEVO

UniProt

Q978W6 - KAE1B_THEVO

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

TV1299

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi107 – 1071IronUniRule annotation
    Metal bindingi111 – 1111IronUniRule annotation
    Metal bindingi128 – 1281IronUniRule annotation
    Binding sitei160 – 1601Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei177 – 1771Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei257 – 2571Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi285 – 2851IronUniRule annotation
    Binding sitei354 – 3541ATPUniRule annotation
    Active sitei445 – 4451Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi333 – 3419ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. protein tyrosine kinase activity Source: InterPro
    7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciTVOL273116:GC31-1320-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:TV1299
    ORF Names:TVG1340828
    OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
    Taxonomic identifieri273116 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001017: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 527527Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000303662Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi273116.TVN1276.

    Structurei

    3D structure databases

    ProteinModelPortaliQ978W6.
    SMRiQ978W6. Positions 1-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini330 – 527198Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 324324Kae1Add
    BLAST
    Regioni128 – 1325Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    KOiK15904.
    OMAiRDNAGMI.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q978W6-1 [UniParc]FASTAAdd to Basket

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    MIVLGLEGTA HTISCGILDE NSIMANVSSM YKPKTGGIHP TQAAAHHVDK    50
    VSEVIAKAIE IAGIKPSDID LVAFSMGPGL GPSLRVTSTA ARTLAVTLKR 100
    PIIGVNHPLG HIEIGKRLSG AQDPVMLYVS GGNTQVIAHL NGRYRVLGET 150
    LDIGIGNMID KFARYAGIPF PGGPEIEKLA KDGRKLLTLP YSVKGMDTSF 200
    SGILTSALEY LKKGEPVEDI SFSIQETAFS MLVEVLERAL YVSGKDEVLM 250
    AGGVALNNRL REMVSEMGRE VDATTYMTDK NYCMDNGAMI AQAGLLMYKS 300
    GIRMNIEDTS INPRYRIDEV DAPWVIEKNV KYRDAGAESR IVNTDFYGRS 350
    AVKKIRIAKG YRLKELDERI RGERMKNEFT VIRRMRDAGI CVPIVYDYDP 400
    FEKTLTLSQI QGELLRDVIR ARPNVMGNVG HDVAVMHKNK ISHGDLTVNN 450
    IIVSDRICFI DASMGKVNAE LEDLAVDVYT LEDSINSLSE NGKTLMKEFK 500
    MSYRANFPQA NDVLNIVEDI RRRHRYV 527
    Length:527
    Mass (Da):58,291
    Last modified:October 1, 2001 - v1
    Checksum:iE66A4EF292645941
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB60441.1.
    RefSeqiNP_111795.1. NC_002689.2.
    WP_010917534.1. NC_002689.2.

    Genome annotation databases

    EnsemblBacteriaiBAB60441; BAB60441; BAB60441.
    GeneIDi1441416.
    KEGGitvo:TVN1276.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000011 Genomic DNA. Translation: BAB60441.1 .
    RefSeqi NP_111795.1. NC_002689.2.
    WP_010917534.1. NC_002689.2.

    3D structure databases

    ProteinModelPortali Q978W6.
    SMRi Q978W6. Positions 1-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273116.TVN1276.

    Protocols and materials databases

    DNASUi 1441416.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB60441 ; BAB60441 ; BAB60441 .
    GeneIDi 1441416.
    KEGGi tvo:TVN1276.

    Phylogenomic databases

    eggNOGi COG0533.
    KOi K15904.
    OMAi RDNAGMI.

    Enzyme and pathway databases

    BioCyci TVOL273116:GC31-1320-MONOMER.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR009220. tRNA_threonyl_synthase/kinase.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

    Entry informationi

    Entry nameiKAE1B_THEVO
    AccessioniPrimary (citable) accession number: Q978W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3