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Q978W6 (KAE1B_THEVO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
  2. Probable serine/threonine-protein kinase BUD32 homolog
    EC=2.7.11.1
Gene names
Ordered Locus Names:TV1299
ORF Names:TVG1340828
OrganismThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) [Complete proteome] [HAMAP]
Taxonomic identifier273116 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma ›

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / YgjD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000303662

Regions

Domain330 – 527198Protein kinase
Nucleotide binding333 – 3419ATP By similarity
Region1 – 324324Kae1 HAMAP-Rule MF_01447

Sites

Active site4451Proton acceptor By similarity
Metal binding1071Iron By similarity
Metal binding1111Iron By similarity
Metal binding1281Iron By similarity
Metal binding2851Iron By similarity
Binding site3541ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q978W6 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: E66A4EF292645941

FASTA52758,291
        10         20         30         40         50         60 
MIVLGLEGTA HTISCGILDE NSIMANVSSM YKPKTGGIHP TQAAAHHVDK VSEVIAKAIE 

        70         80         90        100        110        120 
IAGIKPSDID LVAFSMGPGL GPSLRVTSTA ARTLAVTLKR PIIGVNHPLG HIEIGKRLSG 

       130        140        150        160        170        180 
AQDPVMLYVS GGNTQVIAHL NGRYRVLGET LDIGIGNMID KFARYAGIPF PGGPEIEKLA 

       190        200        210        220        230        240 
KDGRKLLTLP YSVKGMDTSF SGILTSALEY LKKGEPVEDI SFSIQETAFS MLVEVLERAL 

       250        260        270        280        290        300 
YVSGKDEVLM AGGVALNNRL REMVSEMGRE VDATTYMTDK NYCMDNGAMI AQAGLLMYKS 

       310        320        330        340        350        360 
GIRMNIEDTS INPRYRIDEV DAPWVIEKNV KYRDAGAESR IVNTDFYGRS AVKKIRIAKG 

       370        380        390        400        410        420 
YRLKELDERI RGERMKNEFT VIRRMRDAGI CVPIVYDYDP FEKTLTLSQI QGELLRDVIR 

       430        440        450        460        470        480 
ARPNVMGNVG HDVAVMHKNK ISHGDLTVNN IIVSDRICFI DASMGKVNAE LEDLAVDVYT 

       490        500        510        520 
LEDSINSLSE NGKTLMKEFK MSYRANFPQA NDVLNIVEDI RRRHRYV

« Hide

References

[1]"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium."
Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M.
Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000011 Genomic DNA. Translation: BAB60441.1.
RefSeqNP_111795.1. NC_002689.2.

3D structure databases

ProteinModelPortalQ978W6.
SMRQ978W6. Positions 1-325.
ModBaseSearch...

Protein-protein interaction databases

STRING273116.TVN1276.

Protocols and materials databases

DNASU1441416.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB60441; BAB60441; BAB60441.
GeneID1441416.
KEGGtvo:TVN1276.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0533.
KOK15904.
OMAGNCLDQF.
ProtClustDBPRK09605.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR000905. Gcp-like_dom.
IPR022495. Kae1-assoc_kinase_Bud32.
IPR017861. KAE1/YgjD.
IPR022449. Kae1_arc.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERPTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. Kinase_like. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. False negative.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_THEVO
AccessionPrimary (citable) accession number: Q978W6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2001
Last modified: May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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