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Q978W6

- KAE1B_THEVO

UniProt

Q978W6 - KAE1B_THEVO

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Protein
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Gene
TV1299, TVG1340828
Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron By similarity
Metal bindingi111 – 1111Iron By similarity
Metal bindingi128 – 1281Iron By similarity
Binding sitei160 – 1601Threonylcarbamoyl-AMP By similarity
Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei177 – 1771Threonylcarbamoyl-AMP By similarity
Binding sitei257 – 2571Threonylcarbamoyl-AMP By similarity
Metal bindingi285 – 2851Iron By similarity
Binding sitei354 – 3541ATP By similarity
Active sitei445 – 4451Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi333 – 3419ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine kinase activity Source: UniProtKB-KW
  5. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  6. protein tyrosine kinase activity Source: InterPro
  7. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  8. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTVOL273116:GC31-1320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:TV1299
ORF Names:TVG1340828
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001017: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
PRO_0000303662Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi273116.TVN1276.

Structurei

3D structure databases

ProteinModelPortaliQ978W6.
SMRiQ978W6. Positions 1-325.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini330 – 527198Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 324324Kae1UniRule annotation
Add
BLAST
Regioni128 – 1325Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
KOiK15904.
OMAiRDNAGMI.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q978W6-1 [UniParc]FASTAAdd to Basket

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MIVLGLEGTA HTISCGILDE NSIMANVSSM YKPKTGGIHP TQAAAHHVDK    50
VSEVIAKAIE IAGIKPSDID LVAFSMGPGL GPSLRVTSTA ARTLAVTLKR 100
PIIGVNHPLG HIEIGKRLSG AQDPVMLYVS GGNTQVIAHL NGRYRVLGET 150
LDIGIGNMID KFARYAGIPF PGGPEIEKLA KDGRKLLTLP YSVKGMDTSF 200
SGILTSALEY LKKGEPVEDI SFSIQETAFS MLVEVLERAL YVSGKDEVLM 250
AGGVALNNRL REMVSEMGRE VDATTYMTDK NYCMDNGAMI AQAGLLMYKS 300
GIRMNIEDTS INPRYRIDEV DAPWVIEKNV KYRDAGAESR IVNTDFYGRS 350
AVKKIRIAKG YRLKELDERI RGERMKNEFT VIRRMRDAGI CVPIVYDYDP 400
FEKTLTLSQI QGELLRDVIR ARPNVMGNVG HDVAVMHKNK ISHGDLTVNN 450
IIVSDRICFI DASMGKVNAE LEDLAVDVYT LEDSINSLSE NGKTLMKEFK 500
MSYRANFPQA NDVLNIVEDI RRRHRYV 527
Length:527
Mass (Da):58,291
Last modified:October 1, 2001 - v1
Checksum:iE66A4EF292645941
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000011 Genomic DNA. Translation: BAB60441.1.
RefSeqiNP_111795.1. NC_002689.2.
WP_010917534.1. NC_002689.2.

Genome annotation databases

EnsemblBacteriaiBAB60441; BAB60441; BAB60441.
GeneIDi1441416.
KEGGitvo:TVN1276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000011 Genomic DNA. Translation: BAB60441.1 .
RefSeqi NP_111795.1. NC_002689.2.
WP_010917534.1. NC_002689.2.

3D structure databases

ProteinModelPortali Q978W6.
SMRi Q978W6. Positions 1-325.
ModBasei Search...

Protein-protein interaction databases

STRINGi 273116.TVN1276.

Protocols and materials databases

DNASUi 1441416.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB60441 ; BAB60441 ; BAB60441 .
GeneIDi 1441416.
KEGGi tvo:TVN1276.

Phylogenomic databases

eggNOGi COG0533.
KOi K15904.
OMAi RDNAGMI.

Enzyme and pathway databases

BioCyci TVOL273116:GC31-1320-MONOMER.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1.

Entry informationi

Entry nameiKAE1B_THEVO
AccessioniPrimary (citable) accession number: Q978W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2001
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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