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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

TV1299

Organism
Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107IronUniRule annotation1
Metal bindingi111IronUniRule annotation1
Metal bindingi128IronUniRule annotation1
Binding sitei160Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei173Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei177Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei257Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi285IronUniRule annotation1
Binding sitei354ATPUniRule annotation1
Active sitei445Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi333 – 341ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:TV1299
ORF Names:TVG1340828
OrganismiThermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)
Taxonomic identifieri273116 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001017 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036621 – 527Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST527

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi273116.TVN1276.

Structurei

3D structure databases

ProteinModelPortaliQ978W6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini330 – 527Protein kinaseUniRule annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 324Kae1Add BLAST324
Regioni128 – 132Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q978W6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLGLEGTA HTISCGILDE NSIMANVSSM YKPKTGGIHP TQAAAHHVDK
60 70 80 90 100
VSEVIAKAIE IAGIKPSDID LVAFSMGPGL GPSLRVTSTA ARTLAVTLKR
110 120 130 140 150
PIIGVNHPLG HIEIGKRLSG AQDPVMLYVS GGNTQVIAHL NGRYRVLGET
160 170 180 190 200
LDIGIGNMID KFARYAGIPF PGGPEIEKLA KDGRKLLTLP YSVKGMDTSF
210 220 230 240 250
SGILTSALEY LKKGEPVEDI SFSIQETAFS MLVEVLERAL YVSGKDEVLM
260 270 280 290 300
AGGVALNNRL REMVSEMGRE VDATTYMTDK NYCMDNGAMI AQAGLLMYKS
310 320 330 340 350
GIRMNIEDTS INPRYRIDEV DAPWVIEKNV KYRDAGAESR IVNTDFYGRS
360 370 380 390 400
AVKKIRIAKG YRLKELDERI RGERMKNEFT VIRRMRDAGI CVPIVYDYDP
410 420 430 440 450
FEKTLTLSQI QGELLRDVIR ARPNVMGNVG HDVAVMHKNK ISHGDLTVNN
460 470 480 490 500
IIVSDRICFI DASMGKVNAE LEDLAVDVYT LEDSINSLSE NGKTLMKEFK
510 520
MSYRANFPQA NDVLNIVEDI RRRHRYV
Length:527
Mass (Da):58,291
Last modified:October 1, 2001 - v1
Checksum:iE66A4EF292645941
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB60441.1.
RefSeqiWP_010917534.1. NC_002689.2.

Genome annotation databases

EnsemblBacteriaiBAB60441; BAB60441; BAB60441.
GeneIDi1441416.
KEGGitvo:TVG1340828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000011 Genomic DNA. Translation: BAB60441.1.
RefSeqiWP_010917534.1. NC_002689.2.

3D structure databases

ProteinModelPortaliQ978W6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273116.TVN1276.

Protocols and materials databases

DNASUi1441416.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB60441; BAB60441; BAB60441.
GeneIDi1441416.
KEGGitvo:TVG1340828.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAE1B_THEVO
AccessioniPrimary (citable) accession number: Q978W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.