Reviewed, UniProtKB/Swiss-Prot Q978F3 (PGMI_THEVO)

Names and origin

Protein names

Recommended name:
    Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
    Glucose-6-phosphate isomerase
      Short name=GPI
    EC=5.3.1.9
    Phosphoglucose isomerase
      Short name=PGI
    Mannose-6-phosphate isomerase
    EC=5.3.1.8
    Phosphomannose isomerase
      Short name=PMI

Gene names

Ordered Locus Names:	TV1464
ORF Names:	TVG1514630

Organism

Thermoplasma volcanium [Complete proteome] [HAMAP]

Taxonomic identifier

50339 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency By similarity.
Catalytic activity	D-glucose 6-phosphate = D-fructose 6-phosphate. D-mannose 6-phosphate = D-fructose 6-phosphate.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the PGI/PMI family. Contains 1 SIS domain.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Bifunctional phosphoglucose/phosphomannose isomerase		PRO_0000227798
Regions
	Domain	16 – 147	132	SIS
Sites
	Active site	196	1	Proton acceptor By similarity
	Active site	212	1	Proton donor By similarity
	Active site	300	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q978F3-1 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 09BD551B9A286EA6	FASTA	304	34,558
	10 20 30 40 50 60 MQFSEELLTL KDQVRFDKSF KVGKYDKIVI AGMGGSGIAG RIFSEIYDEK PVFLVDDYDI 70 80 90 100 110 120 PSFVDDKTEF IAISYSGNTE ETISASEEAA KKHANVHAIT SGGSLSKMGF DTIIIPSGLQ 130 140 150 160 170 180 PRSSIGYLLM PLVNTFIKPK IGDVDEAYRL LSETDKDNEE EKSIASEIYN GEHIPVIYGS 190 200 210 220 230 240 RPFRAIAYRW KTQFNENAKV LAYSNYFSEL NHNDTMPLKD TYRKDEFYFL VFRSDDSRFS 250 260 270 280 290 300 KRITVTEKIT GNSFRMVDVR GSSLLAKLFY LIHFGDYLTY HLAELRGVDP QDVSLIEKLK KEIA

References

[1]

"Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium." Kawashima T., Amano N., Koike H., Makino S., Higuchi S., Kawashima-Ohya Y., Watanabe K., Yamazaki M., Kanehori K., Kawamoto T., Nunoshiba T., Yamamoto Y., Aramaki H., Makino K., Suzuki M. Proc. Natl. Acad. Sci. U.S.A. 97:14257-14262(2000) [PubMed: 11121031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51530 / DSM 4299 / IFO 15438 / JCM 9571 / GSS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000011 Genomic DNA. Translation: BAB60606.1.
RefSeq	NP_111957.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1442154.
GenomeReviews	Gene locus TV1464 in contig BA000011_GR.
KEGG	tvo:TVN1438.
NMPDR	fig|273116.1.peg.1438.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507538.
OMA	GNTEETL.
Enzyme and pathway databases
BRENDA	5.3.1.8. 256346. 5.3.1.9. 256346.
Family and domain databases
InterPro	IPR011857. Glu6P/Mann6P_isomerase. IPR019490. Glu6P/Mann6P_isomerase_C. IPR001347. SIS. [Graphical view]
Pfam	PF10432. bact-PGI_C. 1 hit. PF01380. SIS. 1 hit. [Graphical view]
TIGRFAMs	TIGR02128. G6PI_arch. 1 hit.
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGMI_THEVO

Accession

Primary (citable) accession number: Q978F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	October 1, 2001
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)