Q976M1 (SYW_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	STK_01690

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus tokodaii

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136734
Regions
	Motif	82 – 90	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	254 – 258	5	"KMSKS" region HAMAP-Rule MF_00140

Sequences

	Sequence		Length	Mass (Da)
	Q976M1 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 109F5A56AF7D7159	FASTA	381	44,718
	10 20 30 40 50 60 MAQDFNVTPW EVKGKVDYDK LIVQFGTQKI TSELKEKIKS IINDELHVML RRDVFFSHRD 70 80 90 100 110 120 LDLVLKDYQD GKGFFLYTGR APSLGMHIGH LIPFIFTKWL QDKFNVNLYI EITDDEKFMR 130 140 150 160 170 180 NPEYTLDQTR QWAYDNILDI IAVGFNPDKT FIFQDTEYIR NMYPIAIKIA KKLTFSEVRA 190 200 210 220 230 240 TFGLDTSSNI GIIWYPALQI APTMFEKRRC LIPAGIDQDP YWRLQRDIAE SLGYYKAAQI 250 260 270 280 290 300 HSKFLPPLTG PEGKMSSSQP ETAIYLTDDP KTVERKIMKY AFSGGQPTIE LHRKYGGNPD 310 320 330 340 350 360 IDVSFQWLYM FFEPDDNKIK KIEEDYRSGA LLTGELKQIL IEKLNDFLEE HRQKREEAKK 370 380 LVNVFKYDGE LAREMWRKIH E

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAK54171.1.
RefSeq	NP_376017.2. NC_003106.2.
3D structure databases
ProteinModelPortal	Q976M1.
ModBase	Search...
Protein-protein interaction databases
STRING	273063.ST0169.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAK54171; BAK54171; STK_01690.
GeneID	1458052.
KEGG	sto:ST0169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000224742.
KO	K01867.
ProtClustDB	PRK12285.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_A. Trp_tRNA_synth_A.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR020653. Tryptophan-tRNA-ligase_arc. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_SULTO

Accession

Primary (citable) accession number: Q976M1
Secondary accession number(s): F9VMM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program