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Protein

Alpha-aminoadipate--LysW ligase LysX

Gene

lysX

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent formation of a covalent bond between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.By similarity

Catalytic activityi

ATP + [lysine-biosynthesis-protein LysW]-C-terminal-L-glutamate + L-2-aminoadipate = ADP + phosphate + [lysine-biosynthesis-protein LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Pathwayi: L-lysine biosynthesis via AAA pathway

This protein is involved in step 1 of the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route).
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Alpha-aminoadipate--LysW ligase LysX (lysX)
  2. Acetylglutamate/acetylaminoadipate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC)
  4. Acetylornithine/acetyl-lysine aminotransferase (argD)
  5. N-acetyl-lysine deacetylase (lysK)
This subpathway is part of the pathway L-lysine biosynthesis via AAA pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-lysine from L-alpha-aminoadipate (Thermus route), the pathway L-lysine biosynthesis via AAA pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911ATPBy similarity
Binding sitei131 – 1311ATPBy similarity
Binding sitei196 – 1961ATPBy similarity
Binding sitei205 – 2051ATPBy similarity
Metal bindingi240 – 2401Magnesium 1By similarity
Metal bindingi253 – 2531Magnesium 1By similarity
Metal bindingi253 – 2531Magnesium 2By similarity
Metal bindingi255 – 2551Magnesium 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi135 – 1417ATPPROSITE-ProRule annotation
Nucleotide bindingi171 – 18212ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-211-MONOMER.
UniPathwayiUPA00033; UER00035.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-aminoadipate--LysW ligase LysX (EC:6.3.2.43)
Short name:
AAA--LysW ligase LysX
Gene namesi
Name:lysX
Ordered Locus Names:STK_01920
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Alpha-aminoadipate--LysW ligase LysXPRO_0000205501Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi273063.ST0192.

Structurei

3D structure databases

ProteinModelPortaliQ976J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 280186ATP-graspPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi262 – 2632N-[TS] motif that is essential for LysX substrate specificity

Sequence similaritiesi

Belongs to the RimK family. LysX subfamily.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01589. Archaea.
COG0189. LUCA.
HOGENOMiHOG000228553.
KOiK05827.
OMAiWKTNTAL.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q976J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILGVIYDLL RWEEKNLIQE ARKLGHTVIP IYTKDFYYFY NNDSNETLGD
60 70 80 90 100
LDVVIQRNTS HARAVITSTI FENLSYKTIN DSSTLIKCEN KLYTLSLLSK
110 120 130 140 150
HGIRVPKTIV AFSKEKALEL ANKLSYPVVI KPVEGSWGRM VARAIDEDTL
160 170 180 190 200
RNFLEYQEYT TLQFRYIYLI QEFVKKPDRD IRIFTIGDEA PVGIYRVNSR
210 220 230 240 250
NWKTNTALGA KAEPLKIDEE LQDLALKVKD IIGGFFLGID VFEDPERGYI
260 270 280
INEVNGVPEY KNTVRVNNFN VSEYLIRKIE EWIKK
Length:285
Mass (Da):33,107
Last modified:December 1, 2001 - v1
Checksum:i6077CF2995B7DFBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB65148.1.
RefSeqiWP_010978130.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB65148; BAB65148; STK_01920.
GeneIDi1458076.
KEGGisto:STK_01920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB65148.1.
RefSeqiWP_010978130.1. NC_003106.2.

3D structure databases

ProteinModelPortaliQ976J9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB65148; BAB65148; STK_01920.
GeneIDi1458076.
KEGGisto:STK_01920.

Phylogenomic databases

eggNOGiarCOG01589. Archaea.
COG0189. LUCA.
HOGENOMiHOG000228553.
KOiK05827.
OMAiWKTNTAL.

Enzyme and pathway databases

UniPathwayiUPA00033; UER00035.
BioCyciSTOK273063:GJC7-211-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLYSX_SULTO
AccessioniPrimary (citable) accession number: Q976J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: December 1, 2001
Last modified: December 9, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.