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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 3 Mg2+ cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei82Important for tRNA non-discriminationUniRule annotation1
Binding sitei167AspartateUniRule annotation1
Binding sitei210AspartateUniRule annotation1
Metal bindingi352Magnesium 2UniRule annotation1
Metal bindingi352Magnesium 3UniRule annotation1
Binding sitei352ATPUniRule annotation1
Metal bindingi355Magnesium 2UniRule annotation1
Binding sitei355AspartateUniRule annotation1
Binding sitei359AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi210 – 212ATPUniRule annotation3
Nucleotide bindingi400 – 403ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:STK_02050
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001110071 – 429Aspartate--tRNA(Asp/Asn) ligaseAdd BLAST429

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST0205.

Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Helixi12 – 14Combined sources3
Beta strandi18 – 31Combined sources14
Beta strandi34 – 41Combined sources8
Beta strandi44 – 50Combined sources7
Helixi57 – 60Combined sources4
Beta strandi68 – 77Combined sources10
Beta strandi79 – 81Combined sources3
Helixi82 – 84Combined sources3
Beta strandi85 – 96Combined sources12
Beta strandi106 – 108Combined sources3
Helixi114 – 119Combined sources6
Helixi121 – 125Combined sources5
Helixi128 – 150Combined sources23
Beta strandi160 – 164Combined sources5
Beta strandi174 – 177Combined sources4
Beta strandi180 – 184Combined sources5
Helixi189 – 199Combined sources11
Beta strandi200 – 209Combined sources10
Beta strandi216 – 218Combined sources3
Beta strandi221 – 231Combined sources11
Helixi235 – 256Combined sources22
Helixi258 – 264Combined sources7
Beta strandi276 – 279Combined sources4
Helixi280 – 289Combined sources10
Helixi302 – 312Combined sources11
Beta strandi315 – 320Combined sources6
Helixi324 – 326Combined sources3
Beta strandi340 – 348Combined sources9
Beta strandi351 – 359Combined sources9
Helixi363 – 372Combined sources10
Helixi377 – 380Combined sources4
Helixi381 – 384Combined sources4
Helixi385 – 388Combined sources4
Beta strandi394 – 400Combined sources7
Helixi401 – 409Combined sources9
Helixi414 – 416Combined sources3
Beta strandi418 – 420Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYDX-ray2.30A/B1-429[»]
ProteinModelPortaliQ976I3.
SMRiQ976I3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ976I3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 192AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00406. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226032.
KOiK01876.
OMAiMENENAP.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF10. PTHR22594:SF10. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q976I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRSHFIADV TPEYDGKEVI WAGWVHLLRD LGGKKFIILR DKTGLGQVVV
60 70 80 90 100
DKNSSAFGIS QELTQESVIQ VRGIVKADKR APRGIELHAE EITLLSKAKA
110 120 130 140 150
PLPLDVSGKV KADIDTRLRE RVLDLRRQEM QAVIKIQSLA LKAFRETLYK
160 170 180 190 200
EGFIEIFTPK IIASATEGGA QLFPVIYFGK EAFLAQSPQL YKELMAGVVE
210 220 230 240 250
RVFEVAPAWR AEESDTPFHL AEFISMDVEM AFADYNDVMQ LLEKILHNIV
260 270 280 290 300
KTIKEEGKEE LKILNYEPPE VKIPIKRLKY TEAIEILRSK GYNIKFGDDI
310 320 330 340 350
GTPELRILNE ELKEDLYFIV DWPSDARPFY TKSKSENPEL SESFDLIYKF
360 370 380 390 400
LEIVSGSTRN HKREVLEEAL KKKGLKPESF EFFLKWFDYG MPPHAGFGMG
410 420
LARLMVMLTG IQSVKEIVPF PRDKKRLTP
Length:429
Mass (Da):49,074
Last modified:December 1, 2001 - v1
Checksum:i5BABA8F4D9437D69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54191.1.
RefSeqiWP_010978146.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54191; BAK54191; STK_02050.
GeneIDi1458094.
KEGGisto:STK_02050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54191.1.
RefSeqiWP_010978146.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYDX-ray2.30A/B1-429[»]
ProteinModelPortaliQ976I3.
SMRiQ976I3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK54191; BAK54191; STK_02050.
GeneIDi1458094.
KEGGisto:STK_02050.

Phylogenomic databases

eggNOGiarCOG00406. Archaea.
COG0017. LUCA.
HOGENOMiHOG000226032.
KOiK01876.
OMAiMENENAP.

Miscellaneous databases

EvolutionaryTraceiQ976I3.

Family and domain databases

HAMAPiMF_02075. Asp_tRNA_synth_type2. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004523. Asp-tRNA_synthase_arc.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF10. PTHR22594:SF10. 1 hit.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00458. aspS_nondisc. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYDND_SULTO
AccessioniPrimary (citable) accession number: Q976I3
Secondary accession number(s): F9VMP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.