Reviewed, UniProtKB/Swiss-Prot Q976G4 (DNLI_SULTO)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	ST0223

Organism

Sulfolobus tokodaii [Complete proteome] [HAMAP]

Taxonomic identifier

111955 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	DNA ligase HAMAP MF_00407		PRO_0000059619
Sites
	Active site	260	1	N6-AMP-lysine intermediate By similarity
	Binding site	258	1	ATP By similarity
	Binding site	265	1	ATP By similarity
	Binding site	280	1	ATP By similarity
	Binding site	310	1	ATP By similarity
	Binding site	350	1	ATP By similarity
	Binding site	427	1	ATP By similarity
	Binding site	433	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q976G4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 9D85DA4458000539	FASTA	600	68,025
	10 20 30 40 50 60 MEFKLIAEYF DKLEKISSRL QLTALLTDLF KKADKNVIDK VVYLIQGKLW PDFLGYPELG 70 80 90 100 110 120 VGEKLLIKAI SIAVNVKEEV VEEQLKVVGD LGEVAMRLKK TPQSASILSF LGAQSNEGLT 130 140 150 160 170 180 VEETYESLTK IALASGEGSR DIKIRSLAGL LKKASPLEAK YIVRFVDGRL RVGIGDATIM 190 200 210 220 230 240 DALSTAFTGS TSFRPLIERA YNLRADLGNI AKIIAQQGVE ALKDIKPQVG IPIRPMLAER 250 260 270 280 290 300 MSDPAEILAK VGGEALVDYK YDGERAQIHK KDKEVYIFSR RLENITRMYP DVVEYVREYI 310 320 330 340 350 360 NANEVIIEGE IVAVDPESNE IRPFQELMHR KRKNDINEAI KEYPVNVYLF DLMLYEDADY 370 380 390 400 410 420 TMKPLPERRK KLEEVIKPND KLHIAHHIYT NNVDKLMEFF YDAISNGAEG VMVKSVAKDS 430 440 450 460 470 480 IYQAGSRGFL WIKLKRDYQS EMADSVDLVV VGAFYGRGKR GGKLSSLLMA AYDPETDTFK 490 500 510 520 530 540 TVCKVASGFS DAELDELQKK LMEIKLDKKD PRVDSQLEPD IWVEPKYVAE IIGAEITLSP 550 560 570 580 590 600 EHTCCKDMVS KGAGLSVRFP RFIRWRDDKS IEDATTPKEI YEMYKMKLRK KEEEQHTDEA

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed: 11572479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000023 Genomic DNA. Translation: BAB65183.1.
RefSeq	NP_376074.1.
3D structure databases
SMR	Q976G4. Positions 1-590.
ModBase	Search...
Genome annotation databases
GeneID	1458113.
GenomeReviews	Gene locus ST0223 in contig BA000023_GR.
KEGG	sto:ST0223.
NMPDR	fig|273063.1.peg.242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q976G4.
OMA	Q976G4. RNWLKLK.
Enzyme and pathway databases
BRENDA	6.5.1.1. 261167.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_SULTO

Accession

Primary (citable) accession number: Q976G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	December 1, 2001
Last modified:	May 5, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)