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Protein

Proteasome-activating nucleotidase

Gene

pan

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi178 – 1836ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome-activating nucleotidaseUniRule annotation
Short name:
PANUniRule annotation
Alternative name(s):
Proteasomal ATPaseUniRule annotation
Proteasome regulatory ATPaseUniRule annotation
Proteasome regulatory particleUniRule annotation
Gene namesi
Name:panUniRule annotation
Ordered Locus Names:STK_03300
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Proteasome-activating nucleotidasePRO_0000084753Add
BLAST

Proteomic databases

PRIDEiQ975U2.

Interactioni

Subunit structurei

Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST0330.

Structurei

3D structure databases

ProteinModelPortaliQ975U2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni390 – 3923Docks into pockets in the proteasome alpha-ring to cause gate openingUniRule annotation

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili19 – 5335UniRule annotationAdd
BLAST

Domaini

Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiarCOG01306. Archaea.
COG1222. LUCA.
HOGENOMiHOG000225146.
KOiK03420.
OMAiEREVNRT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00553. PAN. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q975U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDELDSYRA KHYNSDDPIV RLLEEKIESL TKELEKLRQD LNWYKGELEK
60 70 80 90 100
LLAPPYIEAI VLDILPDGKV IVRSSSGPNL IVNVSSNIDI KKLKPGSLVA
110 120 130 140 150
LTQRGSTIVE VLPEREDAYV KSFEVIEKPN VHYSDIGGLN EQINEIREVI
160 170 180 190 200
ELPLKNPELF KEIGIDPPKG VLLYGPPGTG KTLLAKAVAT ESNATFIQVV
210 220 230 240 250
ASEFAQKFVG EGARIVREVF ELARRKAPSI VFIDEIDAIG AKRVDMGTSG
260 270 280 290 300
EREIQRTLMQ LLAEIDGFKP LDNVKIIAAT NRLDILDPAL LRPGRFDRLI
310 320 330 340 350
EVPLPNFEGR KEIFRIYLQK MKTDGNIRYD ILASMTEGFS GAEIKNVCTE
360 370 380 390
AGYIAIRNGR KYVNMTDLIT AIEKIKAKNN KAKNTDRTEK YV
Length:392
Mass (Da):43,967
Last modified:June 6, 2002 - v2
Checksum:i8F12D91619A2AAF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54240.1.
RefSeqiWP_052846270.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54240; BAK54240; STK_03300.
GeneIDi25400198.
KEGGisto:STK_03300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54240.1.
RefSeqiWP_052846270.1. NC_003106.2.

3D structure databases

ProteinModelPortaliQ975U2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0330.

Proteomic databases

PRIDEiQ975U2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK54240; BAK54240; STK_03300.
GeneIDi25400198.
KEGGisto:STK_03300.

Phylogenomic databases

eggNOGiarCOG01306. Archaea.
COG1222. LUCA.
HOGENOMiHOG000225146.
KOiK03420.
OMAiEREVNRT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00553. PAN. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR023501. Nucleotidase_PAN.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAN_SULTO
AccessioniPrimary (citable) accession number: Q975U2
Secondary accession number(s): F9VMU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: September 7, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.