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Q975R3 (ENDA_SULTO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:STK_03580
OrganismSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]
Taxonomic identifier273063 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01833

Subunit structure

Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180180tRNA-splicing endonuclease HAMAP-Rule MF_01833
PRO_0000109482

Sites

Active site1171 By similarity
Active site1251 By similarity
Active site1561 By similarity

Secondary structure

....................................... 180
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q975R3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 40615B98F1C05085

FASTA18020,507
        10         20         30         40         50         60 
MIGELVKDKI LIKNIEDARL IYKMGYYGKP IGISKPKSAE EINSELILSL IEGVYLVKKG 

        70         80         90        100        110        120 
KLEIVSNGER LDFERLYQIG VTQIPRFRIL YSVYEDLREK GYVVRSGIKY GADFAVYTIG 

       130        140        150        160        170        180 
PGIEHAPYLV IALDENSQIS SNEILGFGRV SHSTRKELIL GIVNLTNGKI RYIMFKWLKM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000023 Genomic DNA. Translation: BAK54248.1.
RefSeqNP_376228.1. NC_003106.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CV8X-ray2.80A/B1-180[»]
ProteinModelPortalQ975R3.
SMRQ975R3. Positions 1-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273063.ST0358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAK54248; BAK54248; STK_03580.
GeneID1458281.
KEGGsto:ST0358.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000107823.
KOK01170.
OMAALKFGSH.

Enzyme and pathway databases

BioCycSTOK273063:GJC7-416-MONOMER.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01833. EndA_short.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR016442. tRNA_splic_arch_short.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ975R3.

Entry information

Entry nameENDA_SULTO
AccessionPrimary (citable) accession number: Q975R3
Secondary accession number(s): F9VMV1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references