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Q975R3

- ENDA_SULTO

UniProt

Q975R3 - ENDA_SULTO

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.UniRule annotation

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei117 – 1171UniRule annotation
    Active sitei125 – 1251UniRule annotation
    Active sitei156 – 1561UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciSTOK273063:GJC7-416-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:STK_03580
    OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
    Taxonomic identifieri273063 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001015: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 180180tRNA-splicing endonucleasePRO_0000109482Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi273063.ST0358.

    Structurei

    Secondary structure

    1
    180
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Beta strandi9 – 124
    Helixi15 – 2511
    Beta strandi28 – 303
    Beta strandi46 – 494
    Helixi50 – 589
    Beta strandi63 – 664
    Beta strandi69 – 713
    Helixi73 – 8311
    Helixi87 – 9913
    Beta strandi103 – 1064
    Helixi108 – 1103
    Beta strandi112 – 1176
    Beta strandi128 – 1347
    Beta strandi138 – 1403
    Helixi141 – 1444
    Beta strandi147 – 1493
    Beta strandi157 – 1637
    Turni165 – 1673
    Beta strandi170 – 1789

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CV8X-ray2.80A/B1-180[»]
    ProteinModelPortaliQ975R3.
    SMRiQ975R3. Positions 1-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ975R3.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000107823.
    KOiK01170.
    OMAiALKFGSH.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01833. EndA_short.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q975R3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIGELVKDKI LIKNIEDARL IYKMGYYGKP IGISKPKSAE EINSELILSL    50
    IEGVYLVKKG KLEIVSNGER LDFERLYQIG VTQIPRFRIL YSVYEDLREK 100
    GYVVRSGIKY GADFAVYTIG PGIEHAPYLV IALDENSQIS SNEILGFGRV 150
    SHSTRKELIL GIVNLTNGKI RYIMFKWLKM 180
    Length:180
    Mass (Da):20,507
    Last modified:December 1, 2001 - v1
    Checksum:i40615B98F1C05085
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAK54248.1.
    RefSeqiNP_376228.1. NC_003106.2.

    Genome annotation databases

    EnsemblBacteriaiBAK54248; BAK54248; STK_03580.
    GeneIDi1458281.
    KEGGisto:ST0358.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAK54248.1 .
    RefSeqi NP_376228.1. NC_003106.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CV8 X-ray 2.80 A/B 1-180 [» ]
    ProteinModelPortali Q975R3.
    SMRi Q975R3. Positions 1-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273063.ST0358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAK54248 ; BAK54248 ; STK_03580 .
    GeneIDi 1458281.
    KEGGi sto:ST0358.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000107823.
    KOi K01170.
    OMAi ALKFGSH.

    Enzyme and pathway databases

    BioCyci STOK273063:GJC7-416-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q975R3.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01833. EndA_short.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR016442. tRNA_splic_arch_short.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005285. tRNA_splic_archaea. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

    Entry informationi

    Entry nameiENDA_SULTO
    AccessioniPrimary (citable) accession number: Q975R3
    Secondary accession number(s): F9VMV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3