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Q975H9 (SYI_SULTO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:STK_04310
OrganismSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]
Taxonomic identifier273063 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus ›

Protein attributes

Sequence length1047 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10471047Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_0000098594

Regions

Motif45 – 5612"HIGH" region HAMAP-Rule MF_02003
Motif594 – 5985"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site5971ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q975H9 [UniParc].

Last modified December 20, 2005. Version 2.
Checksum: 7CE78F48A73547B2

FASTA1,047121,455
        10         20         30         40         50         60 
MPSKFDPLST EKEILKFWEQ NKIYEKLKEY NSKFEKKFLF IDGPPYPSSP IPHIGTVWNK 

        70         80         90        100        110        120 
VIKDCILRYE RLAGFRTYDQ PGYDTHGLPI EVAIEKQLGI QKKSDIIEKI GVDNFVNKCK 

       130        140        150        160        170        180 
EFALNNASSL TANFKNIGIF MDWNNPYMTL HNDYISNSWM LIKKAYERGL LYKGVHVLHW 

       190        200        210        220        230        240 
CPRCETTLAD YEVSEYKEIE DPSIYVKFKV KGSENRYLVI WTTTPWTLPA NVFVMINKDY 

       250        260        270        280        290        300 
EYAEVEVNNE ILIMAKDRVS DVMKEAGIKN YKIVRTFKGV ELVGLQYIHP LADIVTAQKG 

       310        320        330        340        350        360 
LDNYHIVVDA GEEVTLTEGT GLVHSAPGHG DIDFEIGKKI GAPVVMLVND RGEFLPEAGK 

       370        380        390        400        410        420 
YVGKNVRSAA EEIINDLKQR NALLHSGKVV HRYPVCWRCK TPLILRAIEQ WFIGVSKLKE 

       430        440        450        460        470        480 
ELLKEIDNVK WVPDWGKTRI GNMVREIRDW VISRQRFWGT PLPIWICKNC GNIIVIGSRD 

       490        500        510        520        530        540 
ELEKVAITKV PEDLHRPWID KVVVRCNKCG GEAHRVPDVA DVWFDSGVAF FASLGKDWEK 

       550        560        570        580        590        600 
KWKEIGPVDL VLEGHDQLRG WFFSLLRAGV ILLDRAPYNA VLVHGFMLDE QGREMHKSLG 

       610        620        630        640        650        660 
NYVEPSQVTE NEKYGRDVLR LWLLRNTTWE DAKFSWKALD QTKRDLNIIW NVYVFASTYM 

       670        680        690        700        710        720 
NLDNFDPRIY NLDNLKQYLR IEDLWLLSRY NRVVKEVKES MNDFKVHELA NKMTSFIIDD 

       730        740        750        760        770        780 
ISRFYLRLVR KRAWNESNDP DKIAMYYVLY EVLKGSLVLL SPVIPFTTER IYQDFVVDKK 

       790        800        810        820        830        840 
DSVSMESLPE VKEEFINDKI EKEFELSKEI AEAGLNARAK AGIKLRWPIK NAYVFLVSDI 

       850        860        870        880        890        900 
DVEMANNVKE VIKFLLNTKD VEIGGIEGYR RFSKIKATPN RGTIGPDFKR LTNKIVEYIE 

       910        920        930        940        950        960 
NNSEKVASDI IAKGYHEVVI EGEPVQIKVN HVNILEETEE GYISARFNKG VVVLKKEISK 

       970        980        990       1000       1010       1020 
EEEEEGIVRD IIRRIQFMRK QLNVQINDYI LLYIKAPEER TEMLKKWTDY IKSETRAKEV 

      1030       1040 
IIGEVKGDLI LPWEIEDEEY IIGVSKS

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000023 Genomic DNA. Translation: BAK54280.1.
RefSeqNP_376312.2. NC_003106.2.

3D structure databases

ProteinModelPortalQ975H9.
ModBaseSearch...

Protein-protein interaction databases

STRING273063.ST0431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAK54280; BAK54280; STK_04310.
GeneID1458368.
KEGGsto:ST0431.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
ProtClustDBPRK06039.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. tRNAsyn_1a_bind. 2 hits.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_SULTO
AccessionPrimary (citable) accession number: Q975H9
Secondary accession number(s): F9VMY3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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