Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q975H9

- SYI_SULTO

UniProt

Q975H9 - SYI_SULTO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSTOK273063:GJC7-503-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:STK_04310
    OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
    Taxonomic identifieri273063 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001015: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10471047Isoleucine--tRNA ligasePRO_0000098594Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273063.ST0431.

    Structurei

    3D structure databases

    ProteinModelPortaliQ975H9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi45 – 5612"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q975H9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSKFDPLST EKEILKFWEQ NKIYEKLKEY NSKFEKKFLF IDGPPYPSSP     50
    IPHIGTVWNK VIKDCILRYE RLAGFRTYDQ PGYDTHGLPI EVAIEKQLGI 100
    QKKSDIIEKI GVDNFVNKCK EFALNNASSL TANFKNIGIF MDWNNPYMTL 150
    HNDYISNSWM LIKKAYERGL LYKGVHVLHW CPRCETTLAD YEVSEYKEIE 200
    DPSIYVKFKV KGSENRYLVI WTTTPWTLPA NVFVMINKDY EYAEVEVNNE 250
    ILIMAKDRVS DVMKEAGIKN YKIVRTFKGV ELVGLQYIHP LADIVTAQKG 300
    LDNYHIVVDA GEEVTLTEGT GLVHSAPGHG DIDFEIGKKI GAPVVMLVND 350
    RGEFLPEAGK YVGKNVRSAA EEIINDLKQR NALLHSGKVV HRYPVCWRCK 400
    TPLILRAIEQ WFIGVSKLKE ELLKEIDNVK WVPDWGKTRI GNMVREIRDW 450
    VISRQRFWGT PLPIWICKNC GNIIVIGSRD ELEKVAITKV PEDLHRPWID 500
    KVVVRCNKCG GEAHRVPDVA DVWFDSGVAF FASLGKDWEK KWKEIGPVDL 550
    VLEGHDQLRG WFFSLLRAGV ILLDRAPYNA VLVHGFMLDE QGREMHKSLG 600
    NYVEPSQVTE NEKYGRDVLR LWLLRNTTWE DAKFSWKALD QTKRDLNIIW 650
    NVYVFASTYM NLDNFDPRIY NLDNLKQYLR IEDLWLLSRY NRVVKEVKES 700
    MNDFKVHELA NKMTSFIIDD ISRFYLRLVR KRAWNESNDP DKIAMYYVLY 750
    EVLKGSLVLL SPVIPFTTER IYQDFVVDKK DSVSMESLPE VKEEFINDKI 800
    EKEFELSKEI AEAGLNARAK AGIKLRWPIK NAYVFLVSDI DVEMANNVKE 850
    VIKFLLNTKD VEIGGIEGYR RFSKIKATPN RGTIGPDFKR LTNKIVEYIE 900
    NNSEKVASDI IAKGYHEVVI EGEPVQIKVN HVNILEETEE GYISARFNKG 950
    VVVLKKEISK EEEEEGIVRD IIRRIQFMRK QLNVQINDYI LLYIKAPEER 1000
    TEMLKKWTDY IKSETRAKEV IIGEVKGDLI LPWEIEDEEY IIGVSKS 1047
    Length:1,047
    Mass (Da):121,455
    Last modified:December 20, 2005 - v2
    Checksum:i7CE78F48A73547B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAK54280.1.
    RefSeqiNP_376312.2. NC_003106.2.

    Genome annotation databases

    EnsemblBacteriaiBAK54280; BAK54280; STK_04310.
    GeneIDi1458368.
    KEGGisto:ST0431.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAK54280.1 .
    RefSeqi NP_376312.2. NC_003106.2.

    3D structure databases

    ProteinModelPortali Q975H9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273063.ST0431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAK54280 ; BAK54280 ; STK_04310 .
    GeneIDi 1458368.
    KEGGi sto:ST0431.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.

    Enzyme and pathway databases

    BioCyci STOK273063:GJC7-503-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

    Entry informationi

    Entry nameiSYI_SULTO
    AccessioniPrimary (citable) accession number: Q975H9
    Secondary accession number(s): F9VMY3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3