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Q975H9

- SYI_SULTO

UniProt

Q975H9 - SYI_SULTO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-503-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:STK_04310
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001015: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Isoleucine--tRNA ligasePRO_0000098594Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST0431.

Structurei

3D structure databases

ProteinModelPortaliQ975H9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5612"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q975H9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSKFDPLST EKEILKFWEQ NKIYEKLKEY NSKFEKKFLF IDGPPYPSSP
60 70 80 90 100
IPHIGTVWNK VIKDCILRYE RLAGFRTYDQ PGYDTHGLPI EVAIEKQLGI
110 120 130 140 150
QKKSDIIEKI GVDNFVNKCK EFALNNASSL TANFKNIGIF MDWNNPYMTL
160 170 180 190 200
HNDYISNSWM LIKKAYERGL LYKGVHVLHW CPRCETTLAD YEVSEYKEIE
210 220 230 240 250
DPSIYVKFKV KGSENRYLVI WTTTPWTLPA NVFVMINKDY EYAEVEVNNE
260 270 280 290 300
ILIMAKDRVS DVMKEAGIKN YKIVRTFKGV ELVGLQYIHP LADIVTAQKG
310 320 330 340 350
LDNYHIVVDA GEEVTLTEGT GLVHSAPGHG DIDFEIGKKI GAPVVMLVND
360 370 380 390 400
RGEFLPEAGK YVGKNVRSAA EEIINDLKQR NALLHSGKVV HRYPVCWRCK
410 420 430 440 450
TPLILRAIEQ WFIGVSKLKE ELLKEIDNVK WVPDWGKTRI GNMVREIRDW
460 470 480 490 500
VISRQRFWGT PLPIWICKNC GNIIVIGSRD ELEKVAITKV PEDLHRPWID
510 520 530 540 550
KVVVRCNKCG GEAHRVPDVA DVWFDSGVAF FASLGKDWEK KWKEIGPVDL
560 570 580 590 600
VLEGHDQLRG WFFSLLRAGV ILLDRAPYNA VLVHGFMLDE QGREMHKSLG
610 620 630 640 650
NYVEPSQVTE NEKYGRDVLR LWLLRNTTWE DAKFSWKALD QTKRDLNIIW
660 670 680 690 700
NVYVFASTYM NLDNFDPRIY NLDNLKQYLR IEDLWLLSRY NRVVKEVKES
710 720 730 740 750
MNDFKVHELA NKMTSFIIDD ISRFYLRLVR KRAWNESNDP DKIAMYYVLY
760 770 780 790 800
EVLKGSLVLL SPVIPFTTER IYQDFVVDKK DSVSMESLPE VKEEFINDKI
810 820 830 840 850
EKEFELSKEI AEAGLNARAK AGIKLRWPIK NAYVFLVSDI DVEMANNVKE
860 870 880 890 900
VIKFLLNTKD VEIGGIEGYR RFSKIKATPN RGTIGPDFKR LTNKIVEYIE
910 920 930 940 950
NNSEKVASDI IAKGYHEVVI EGEPVQIKVN HVNILEETEE GYISARFNKG
960 970 980 990 1000
VVVLKKEISK EEEEEGIVRD IIRRIQFMRK QLNVQINDYI LLYIKAPEER
1010 1020 1030 1040
TEMLKKWTDY IKSETRAKEV IIGEVKGDLI LPWEIEDEEY IIGVSKS
Length:1,047
Mass (Da):121,455
Last modified:December 20, 2005 - v2
Checksum:i7CE78F48A73547B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000023 Genomic DNA. Translation: BAK54280.1.
RefSeqiNP_376312.2. NC_003106.2.
WP_010978404.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54280; BAK54280; STK_04310.
GeneIDi1458368.
KEGGisto:ST0431.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000023 Genomic DNA. Translation: BAK54280.1 .
RefSeqi NP_376312.2. NC_003106.2.
WP_010978404.1. NC_003106.2.

3D structure databases

ProteinModelPortali Q975H9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273063.ST0431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAK54280 ; BAK54280 ; STK_04310 .
GeneIDi 1458368.
KEGGi sto:ST0431.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.

Enzyme and pathway databases

BioCyci STOK273063:GJC7-503-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Entry informationi

Entry nameiSYI_SULTO
AccessioniPrimary (citable) accession number: Q975H9
Secondary accession number(s): F9VMY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3