Q975G5 (PSA_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PsmA

Gene names

Name:	psmA
Ordered Locus Names:	STK_04460

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus tokodaii

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation By similarity. HAMAP-Rule MF_00289
Catalytic activity	Cleavage of peptide bonds with very broad specificity. HAMAP-Rule MF_00289
Enzyme regulation	The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. HAMAP-Rule MF_00289
Subunit structure	The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00289.
Sequence similarities	Belongs to the peptidase T1A family.
Sequence caution	The sequence BAB65436.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Proteasome
Molecular function	Hydrolase Protease Threonine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular_function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Proteasome subunit alpha HAMAP-Rule MF_00289		PRO_0000124188

Sequences

	Sequence		Length	Mass (Da)
	Q975G5 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 7CAD8AAD4CCF8C19	FASTA	242	26,667
	10 20 30 40 50 60 MAFGPAAMGY DRAITIFSPD GSLYQVDYAF EAVKKGWTTL GVKTKAGVVL IGEKRKATQL 70 80 90 100 110 120 LDVDSIEKVF ILDDHVGCSF AGLASDGRIL IDYARSQALQ HRLIYDEPIN IDYLTKLVSD 130 140 150 160 170 180 VKQMYTQHGG VRPFGVALII GGIDRGKTPK LLMTEPSGQF MPYYAVAIGQ GGYTATEYFE 190 200 210 220 230 240 KNYREDLNMQ DTILLGIRAL ASTLKPGEKL APSNIEVGFA DVDSGMFRKM SFEERASILQ KL

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAB65436.1. Different initiation.
RefSeq	NP_376327.1. NC_003106.2.
3D structure databases
ProteinModelPortal	Q975G5.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	273063.ST0446.
Protein family/group databases
MEROPS	T01.973.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB65436; BAB65436; STK_04460.
GeneID	1458383.
KEGG	sto:ST0446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0638.
HOGENOM	HOG000091085.
KO	K03432.
Enzyme and pathway databases
BioCyc	STOK273063:GJC7-519-MONOMER.
Family and domain databases
Gene3D	3.60.20.10. 1 hit.
HAMAP	MF_00289_A. Proteasome_A_A.
InterPro	IPR029055. Ntn_hydrolases_N. IPR019982. Proteasome_asu_arc. IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
SUPFAM	SSF56235. SSF56235. 1 hit.
TIGRFAMs	TIGR03633. arc_protsome_A. 1 hit.
PROSITE	PS00388. PROTEASOME_ALPHA_1. 1 hit. PS51475. PROTEASOME_ALPHA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA_SULTO

Accession

Primary (citable) accession number: Q975G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	June 11, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program