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Protein

CCA-adding enzyme

Gene

cca

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATP or CTPUniRule annotation
Binding sitei45 – 451ATP or CTPUniRule annotation
Metal bindingi54 – 541MagnesiumUniRule annotation
Metal bindingi56 – 561MagnesiumUniRule annotation
Metal bindingi107 – 1071MagnesiumUniRule annotation
Binding sitei130 – 1301ATP or CTPUniRule annotation
Binding sitei150 – 1501ATP or CTPUniRule annotation
Binding sitei159 – 1591ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1054-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:STK_09520
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 413413CCA-adding enzymePRO_0000139084Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST0952.

Structurei

3D structure databases

ProteinModelPortaliQ973E7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiKGTWLKG.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR006116. 2-5-oligoadenylate_synth_N.
IPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q973E7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIEEEVLKR IKPSKEDEEK LRERAQIILD RLKGYNAEIE GSFRKGTWLK
60 70 80 90 100
GDTDIDIFVF FPKSVGKEYL REKALKELIE RFRDLNYKIA YAEHPYLIVY
110 120 130 140 150
VDDVEIDVVP ALSIESGEEA ITAADRTPFH TKYVISHLDE KGRDEVRLLK
160 170 180 190 200
RFLKGIGVYG AEIKVKGFSG YVTELLIIYY GSFKEVLRNA SKFRPPVRIE
210 220 230 240 250
LVRPKKEFDS PLIVPDPVDP KRNASSAVSL KSLATFALAS KIYLEKPSME
260 270 280 290 300
FFFPSKPGRQ TIKGDILLVK VKIEESTVED IIWGQVWRNV EKLKTLISHE
310 320 330 340 350
GYRVIDISAW GDAENITIGI QLESKSIGEY YLNVGPYFYL HNVKNFIEEN
360 370 380 390 400
ENVWIGEDGR LYSIKRRRYT VEEIIKRNLS FKQKFTYELQ WLTEEVDDPW
410
INLFLRKTPS WLK
Length:413
Mass (Da):48,137
Last modified:December 1, 2001 - v1
Checksum:i3FA5FDE72A5AAC42
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54422.1.
RefSeqiWP_010978948.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54422; BAK54422; STK_09520.
GeneIDi1458917.
KEGGisto:STK_09520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54422.1.
RefSeqiWP_010978948.1. NC_003106.2.

3D structure databases

ProteinModelPortaliQ973E7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK54422; BAK54422; STK_09520.
GeneIDi1458917.
KEGGisto:STK_09520.

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiKGTWLKG.

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1054-MONOMER.

Family and domain databases

HAMAPiMF_01264. CCA_arch.
InterProiIPR006116. 2-5-oligoadenylate_synth_N.
IPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Entry informationi

Entry nameiCCA_SULTO
AccessioniPrimary (citable) accession number: Q973E7
Secondary accession number(s): F9VNR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 1, 2001
Last modified: December 9, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.