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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei120 – 1201Nucleophile; methyl group acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1076-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:STK_09670
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156Methylated-DNA--protein-cysteine methyltransferasePRO_0000139388Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273063.ST0967.

Structurei

Secondary structure

1
156
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Beta strandi12 – 187Combined sources
Beta strandi23 – 297Combined sources
Helixi34 – 363Combined sources
Helixi39 – 424Combined sources
Helixi43 – 5210Combined sources
Turni53 – 553Combined sources
Helixi71 – 8010Combined sources
Helixi91 – 977Combined sources
Helixi102 – 1109Combined sources
Turni115 – 1173Combined sources
Helixi120 – 1223Combined sources
Beta strandi126 – 1283Combined sources
Helixi136 – 14510Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WRJX-ray2.00A1-156[»]
ProteinModelPortaliQ973C7.
SMRiQ973C7. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ973C7.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiDEYFAGK.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q973C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVYGLYKSP FGPITVAKNE KGFVMLDFCD CAERSSLDND YFTDFFYKLD
60 70 80 90 100
LYFEGKKVDL TEPVDFKPFN EFRIRVFKEV MRIKWGEVRT YKQVADAVKT
110 120 130 140 150
SPRAVGTALS KNNVLLIIPC HRVIGEKSLG GYSRGVELKR KLLELEGIDV

AKFIEK
Length:156
Mass (Da):17,950
Last modified:December 1, 2001 - v1
Checksum:i07239C0E302A25AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54430.1.
RefSeqiWP_010978968.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54430; BAK54430; STK_09670.
GeneIDi1458939.
KEGGisto:STK_09670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54430.1.
RefSeqiWP_010978968.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WRJX-ray2.00A1-156[»]
ProteinModelPortaliQ973C7.
SMRiQ973C7. Positions 1-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK54430; BAK54430; STK_09670.
GeneIDi1458939.
KEGGisto:STK_09670.

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiDEYFAGK.

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1076-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ973C7.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
  2. "Crystal structure of O6-methylguanine methyltransferase from Sulfolobus tokodaii."
    Kuroda M., Tsunoda M., Nakamura K.T.
    Submitted (OCT-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS).

Entry informationi

Entry nameiOGT_SULTO
AccessioniPrimary (citable) accession number: Q973C7
Secondary accession number(s): F9VNS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: December 9, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.