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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei120Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:STK_09670
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393881 – 156Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST156

Interactioni

Protein-protein interaction databases

STRINGi273063.ST0967.

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Beta strandi12 – 18Combined sources7
Beta strandi23 – 29Combined sources7
Helixi34 – 36Combined sources3
Helixi39 – 42Combined sources4
Helixi43 – 52Combined sources10
Turni53 – 55Combined sources3
Helixi71 – 80Combined sources10
Helixi91 – 97Combined sources7
Helixi102 – 110Combined sources9
Turni115 – 117Combined sources3
Helixi120 – 122Combined sources3
Beta strandi126 – 128Combined sources3
Helixi136 – 145Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WRJX-ray2.00A1-156[»]
ProteinModelPortaliQ973C7.
SMRiQ973C7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ973C7.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiLCKIPFG.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q973C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVYGLYKSP FGPITVAKNE KGFVMLDFCD CAERSSLDND YFTDFFYKLD
60 70 80 90 100
LYFEGKKVDL TEPVDFKPFN EFRIRVFKEV MRIKWGEVRT YKQVADAVKT
110 120 130 140 150
SPRAVGTALS KNNVLLIIPC HRVIGEKSLG GYSRGVELKR KLLELEGIDV

AKFIEK
Length:156
Mass (Da):17,950
Last modified:December 1, 2001 - v1
Checksum:i07239C0E302A25AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54430.1.
RefSeqiWP_010978968.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAK54430; BAK54430; STK_09670.
GeneIDi1458939.
KEGGisto:STK_09670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAK54430.1.
RefSeqiWP_010978968.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WRJX-ray2.00A1-156[»]
ProteinModelPortaliQ973C7.
SMRiQ973C7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST0967.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK54430; BAK54430; STK_09670.
GeneIDi1458939.
KEGGisto:STK_09670.

Phylogenomic databases

eggNOGiarCOG02724. Archaea.
COG0350. LUCA.
HOGENOMiHOG000244137.
KOiK00567.
OMAiLCKIPFG.

Miscellaneous databases

EvolutionaryTraceiQ973C7.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00772. OGT. 1 hit.
InterProiIPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR008332. MethylG_MeTrfase_N.
IPR023546. MGMT.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiPS00374. MGMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGT_SULTO
AccessioniPrimary (citable) accession number: Q973C7
Secondary accession number(s): F9VNS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.