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Protein

Diphthine synthase

Gene

dphB

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.UniRule annotation

Catalytic activityi

3 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2].UniRule annotation

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei86S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei89S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei166S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei207S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei232S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine synthaseUniRule annotation (EC:2.1.1.98UniRule annotation)
Alternative name(s):
Diphthamide biosynthesis methyltransferaseUniRule annotation
Gene namesi
Name:dphBUniRule annotation
Ordered Locus Names:STK_12780
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001561291 – 254Diphthine synthaseAdd BLAST254

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273063.ST1278.

Structurei

3D structure databases

ProteinModelPortaliQ971V1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni114 – 115S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diphthine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04161. Archaea.
COG1798. LUCA.
HOGENOMiHOG000205302.
KOiK20215.
OMAiGGDTMVS.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q971V1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTLKLIGLG LSAKFVTREA IDEISKCNVV LFESYTSLSC DINLDFIKFL
60 70 80 90 100
NKNVIIVDRK FIENNIKEII KLLKEKEDVC IVTIGDPMIA TTHVSLIVEV
110 120 130 140 150
KDKGYNFKVI PGISVHCYII SKSMLSSYKF GKSVTITYPY NNKIDTTPYD
160 170 180 190 200
VIYDNFIRGL HTILYLDLKE DKIMTAKEAV ELLIEMEKIK KQGLVSDDRI
210 220 230 240 250
IIVGQRLGCD DEEVVALRLK EVFNYKFKEP PHIIVFPTDK LHFMEVEALK

CLMK
Length:254
Mass (Da):29,136
Last modified:December 1, 2001 - v1
Checksum:i5AE69909327DFB8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66319.1.
RefSeqiWP_010979297.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB66319; BAB66319; STK_12780.
GeneIDi1459277.
KEGGisto:STK_12780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66319.1.
RefSeqiWP_010979297.1. NC_003106.2.

3D structure databases

ProteinModelPortaliQ971V1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST1278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB66319; BAB66319; STK_12780.
GeneIDi1459277.
KEGGisto:STK_12780.

Phylogenomic databases

eggNOGiarCOG04161. Archaea.
COG1798. LUCA.
HOGENOMiHOG000205302.
KOiK20215.
OMAiGGDTMVS.

Enzyme and pathway databases

UniPathwayiUPA00559.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPHB_SULTO
AccessioniPrimary (citable) accession number: Q971V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.