Reviewed, UniProtKB/Swiss-Prot Q971K2 (G3P_SULTO)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	ST1356

Organism

Sulfolobus tokodaii [Complete proteome] [HAMAP]

Taxonomic identifier

111955 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145736
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	303	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q971K2-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: F5620C2D7A673303	FASTA	343	37,515
	10 20 30 40 50 60 MEKVKVAVNG YGTIGKRVAD AIRLQPDMEL IGVGKTSPNY EAIIADKKGI KIYTVKDNIG 70 80 90 100 110 120 KFEKSGIRVA GTIEDMVKEA DIVVDTTPNG VGATYKPLYQ SLGKNALFQG GEKADVADIS 130 140 150 160 170 180 FSALCNYDEA KGKKYIRVVS CNTTGMLRII CTMNKISKVE KVRATIVRRA ADPKEVKRGP 190 200 210 220 230 240 INSIVPDPAS VPSHHAKDVL TVIKGIDIVT MAVIAPTTLM HLHTMVLTVK DKVNRDDIIN 250 260 270 280 290 300 TFLNTPRIIL VNSSRTTVSS TAEIIEVSRD MGRVRYDIPE VVVFEDSIYT NGNEVFLMYG 310 320 330 340 VHQESIVVPE NIDAIRASLG LMGKEESIKV TNDTLGIMKG YLL

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed: 11572479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000023 Genomic DNA. Translation: BAB66418.1.
RefSeq	NP_377309.1.
3D structure databases
HSSP	HSSP built from PDB template 1B7G based on UniProtKB P39460.
ModBase	Search...
Genome annotation databases
GeneID	1459378.
GenomeReviews	Gene locus ST1356 in contig BA000023_GR.
KEGG	sto:ST1356.
NMPDR	fig|273063.1.peg.1477.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q971K2.
OMA	Q971K2. AIFQGGE.
Enzyme and pathway databases
BRENDA	1.2.1.59. 261167.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_SULTO

Accession

Primary (citable) accession number: Q971K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)