Q971D0 (SYE_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	STK_14230

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus tokodaii

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000119732
Regions
	Motif	105 – 115	11	"HIGH" region HAMAP-Rule MF_00022

Sequences

	Sequence		Length	Mass (Da)
	Q971D0 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 5741806D8D2482CF	FASTA	566	65,517
	10 20 30 40 50 60 MEEIEELIYK YALQNAYKHG GKAQDKAVVS KIFVERPDLR SRAKEISEIA KKIVEKVNSM 70 80 90 100 110 120 SIQDQERELK EKYPDLLEEK KKEEPEKKTL PPIKVEGKFV TRFAPNPDGP IHLGNARAAI 130 140 150 160 170 180 ISYKYAEMYK GEFILRFDDT DPKVKKPIKE AYDWIRKDLK WLGIKWDKEV KASERLEFYY 190 200 210 220 230 240 SMAKELISKG FAYVDTCSEE EFKKMRDASK PCPNRLKSAE DNLYLFEKML NGEFKEGEAV 250 260 270 280 290 300 VRIKTDLSLP DPSQRDWVLL RIIDVKKNPH PITGDKYWIW PTYNFASALD DHDLGITHIF 310 320 330 340 350 360 RGKEHEVNAE KQKWIYNYMG WKYPYVEEFG RLRLEGFMMS KSKIRTVLEK GVSIDDPRLP 370 380 390 400 410 420 TLAGLRRRGI LPETIIETII TVGLKVSDAT ISFDNLAALN RKKLDPIAKR LMFVQQPKEF 430 440 450 460 470 480 IIELSEPIRA KIPYNPSKPS EYREILVNPG DKILLDAKDA EEGAVVRLME LCNVTISGNK 490 500 510 520 530 540 LIFHSKTLED AKKLNAKIIQ WVKKDEASNV EVIIPDPNEE KPPISGYGEK EIGNLKINEI 550 560 VQFIRFGFVR VDNKIDNKVT VIFSHE

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAK54565.1.
RefSeq	NP_377381.1. NC_003106.2.
3D structure databases
ProteinModelPortal	Q971D0.
ModBase	Search...
Protein-protein interaction databases
STRING	273063.ST1423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAK54565; BAK54565; STK_14230.
GeneID	1459453.
KEGG	sto:ST1423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000073585.
KO	K01885.
OMA	MRFAPNP.
ProtClustDB	PRK04156.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_A. Glu_tRNA_synth_A.
InterPro	IPR004526. Glu-tRNA-synth_Ib_arc/euk. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR022888. Glu_tRNA_synth_arc. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00463. gltX_arch. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SULTO

Accession

Primary (citable) accession number: Q971D0
Secondary accession number(s): F9VP57

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program