Q970V6 (PURL_SULTO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	STK_14950

Organism

Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]

Taxonomic identifier

273063 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100526
Regions
	Nucleotide binding	95 – 106	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q970V6 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DDCC43B6C9A5883C	FASTA	703	76,996
	10 20 30 40 50 60 MKITLSSYEM ELVRKKLARE PNEAEWLTID ALWSEHCSYK SSKVFLRSFP SEGEKVLMGI 70 80 90 100 110 120 EDWQDAGALD VGDGWAIVLK LESHNHPSAI DPFNGAATGV GGIIRDIISK GAKPIALLDM 130 140 150 160 170 180 IRVGNLSNPR NKWLLKNIIA GIGFYGNSIG VPVVGGELDF DDSYNDNPLV DVAGVGIVRK 190 200 210 220 230 240 DKIVPSVVKE PGLKIVIVGL TGLDGLGGAS FASRKLSGED EIGAVQIADP FAGKIVLDVT 250 260 270 280 290 300 LEIADKVEAI KDLGGGGLVV GVTEMANGLG AIVNLDKVPL RVKDLKPEEI LVSETQERML 310 320 330 340 350 360 FAVKEENVNE VCKAFEYYDY PCAVIGEFVK EPYIKFLYGG KEIVSLPSDL LLSPPRFIWE 370 380 390 400 410 420 IKKPKLIKSD KKPEVGLEES IRAILSRIIS KEWAYSQFDY EVGTSTVLKP GEADSALISL 430 440 450 460 470 480 PNGKLLALKG DANPDLCAED SYECGKSIVA EAYRNLASVG AIGIGVVDHL QFGDPKKPEV 490 500 510 520 530 540 YYSFVEAIRG IAEASKFFST PIVGGKVSFY NENKEGKAIK PTPLIVMAGL IKDKFLRNKV 550 560 570 580 590 600 VEDSYITLIG FTRDEMRGSL FGKIFGNYGE VPKARLNEDY LASQLVVNLI NDEKIFFAKD 610 620 630 640 650 660 INKGGLIASL FSILVKGMGV EIETSSIPSD TDDWIPKLYS ENGGRFIVLT NDPEYIIRKS 670 680 690 700 KGIHISVIGK ITKDQGIIKI DNKEINVNKE IDNYYNYLYE VMS

References

[1]

"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7." Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H. DNA Res. 8:123-140(2001) [PubMed: 11572479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000023 Genomic DNA. Translation: BAB66567.1.
RefSeq	NP_377458.1. NC_003106.2.
3D structure databases
ProteinModelPortal	Q970V6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1459531.
GenomeReviews	Gene locus ST1495 in contig BA000023_GR.
KEGG	sto:ST1495.
NMPDR	fig|273063.1.peg.1626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q970V6.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	STOK273063:ST1495-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_SULTO

Accession

Primary (citable) accession number: Q970V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	December 1, 2001
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program