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Protein

Glutamate--LysW ligase ArgX

Gene

argX

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.By similarity

Catalytic activityi

[LysW] + L-glutamate + ATP = [LysW]-L-glutamate + ADP + phosphate + H+.By similarity

Cofactori

Mg2+1 PublicationNote: Binds 2 magnesium ions per subunit.1 Publication

Pathwayi: L-arginine biosynthesis

This protein is involved in the pathway L-arginine biosynthesis, which is part of Amino-acid biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87ATP1 Publication1
Binding sitei127ATP1 Publication1
Binding sitei192Substrate1 Publication1
Binding sitei202ATP1 Publication1
Metal bindingi237Magnesium 11 Publication1
Metal bindingi250Magnesium 11 Publication1
Metal bindingi250Magnesium 21 Publication1
Metal bindingi252Magnesium 21 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi131 – 137ATP1 Publication7
Nucleotide bindingi167 – 178ATP1 PublicationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.B19. 6166.
UniPathwayiUPA00068.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--LysW ligase ArgXCurated (EC:6.3.2.-By similarity)
Gene namesi
Name:argX1 Publication
Synonyms:argE
Ordered Locus Names:STK_15050
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004229901 – 282Glutamate--LysW ligase ArgXAdd BLAST282

Interactioni

Subunit structurei

Homotetramer. Interacts with LysW.1 Publication

Protein-protein interaction databases

DIPiDIP-61747N.
STRINGi273063.ST1505.

Structurei

Secondary structure

1282
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Helixi12 – 23Combined sources12
Beta strandi27 – 32Combined sources6
Turni33 – 35Combined sources3
Beta strandi38 – 41Combined sources4
Helixi42 – 46Combined sources5
Beta strandi48 – 52Combined sources5
Helixi57 – 69Combined sources13
Beta strandi73 – 76Combined sources4
Helixi78 – 84Combined sources7
Helixi87 – 96Combined sources10
Beta strandi104 – 107Combined sources4
Helixi110 – 120Combined sources11
Beta strandi122 – 127Combined sources6
Turni133 – 136Combined sources4
Beta strandi138 – 140Combined sources3
Helixi143 – 153Combined sources11
Helixi159 – 162Combined sources4
Beta strandi164 – 168Combined sources5
Beta strandi172 – 182Combined sources11
Beta strandi185 – 193Combined sources9
Helixi203 – 205Combined sources3
Beta strandi208 – 210Combined sources3
Helixi216 – 229Combined sources14
Beta strandi232 – 241Combined sources10
Turni242 – 244Combined sources3
Beta strandi245 – 254Combined sources10
Helixi258 – 264Combined sources7
Helixi268 – 280Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VPBX-ray1.80A/B/C/D1-282[»]
3VPCX-ray1.87A/B/C/D1-282[»]
ProteinModelPortaliQ970U6.
SMRiQ970U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 277ATP-graspPROSITE-ProRule annotationAdd BLAST187

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni203 – 204Substrate binding1 Publication2
Regioni256 – 260Substrate binding1 Publication5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi259 – 260GF motif that is essential for ArgX substrate specificity1 Publication2

Sequence similaritiesi

Belongs to the RimK family. LysX subfamily.Curated

Phylogenomic databases

eggNOGiarCOG01589. Archaea.
COG0189. LUCA.
HOGENOMiHOG000228553.
KOiK19412.
OMAiINVAQEP.
OrthoDBiPOG093Z07VM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
PfamiView protein in Pfam
PF08443. RimK. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q970U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVVLIVDIV RQEEKLIAKA LEENKVQYDI INVAQEPLPF NKALGRYDVA
60 70 80 90 100
IIRPVSMYRA LYSSAVLEAA GVHTINSSDV INVCGDKILT YSKLYREGIP
110 120 130 140 150
IPDSIIALSA EAALKAYEQR GFPLIDKPPI GSWGRLVSLI RDVFEGKTII
160 170 180 190 200
EHRELMGNSA LKAHIVQEYI QYKGRDIRCI AIGEELLGCY ARNIPPNEWR
210 220 230 240 250
ANVALGGTPS NIEVDEKLKE TVVKAVSIVH GEFVSIDILE HPNKGYVVNE
260 270 280
LNDVPEFKGF MVATNINVAQ KLVEYIKENY SK
Length:282
Mass (Da):31,534
Last modified:December 1, 2001 - v1
Checksum:iB1690D7F15D6E75F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66577.1.
RefSeqiWP_010979555.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB66577; BAB66577; STK_15050.
GeneIDi1459541.
KEGGisto:STK_15050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66577.1.
RefSeqiWP_010979555.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VPBX-ray1.80A/B/C/D1-282[»]
3VPCX-ray1.87A/B/C/D1-282[»]
ProteinModelPortaliQ970U6.
SMRiQ970U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61747N.
STRINGi273063.ST1505.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB66577; BAB66577; STK_15050.
GeneIDi1459541.
KEGGisto:STK_15050.

Phylogenomic databases

eggNOGiarCOG01589. Archaea.
COG0189. LUCA.
HOGENOMiHOG000228553.
KOiK19412.
OMAiINVAQEP.
OrthoDBiPOG093Z07VM.

Enzyme and pathway databases

UniPathwayiUPA00068.
BRENDAi6.3.2.B19. 6166.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
PfamiView protein in Pfam
PF08443. RimK. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARGX_SULTO
AccessioniPrimary (citable) accession number: Q970U6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: December 1, 2001
Last modified: May 10, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.