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Q970U6 (ARGX_SULTO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--LysW ligase ArgX

EC=6.3.2.-
Gene names
Name:argE
Synonyms:argX
Ordered Locus Names:STK_15050
OrganismSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) [Complete proteome] [HAMAP]
Taxonomic identifier273063 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW By similarity.

Cofactor

Binds 2 magnesium ions per subunit.

Subunit structure

Homodimer By similarity. Homotetramer. Interacts with LysW. Ref.2

Sequence similarities

Belongs to the RimK family. LysX subfamily.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Glutamate--LysW ligase ArgX
PRO_0000422990

Regions

Domain91 – 277187ATP-grasp
Nucleotide binding131 – 1377ATP
Nucleotide binding167 – 17812ATP
Region203 – 2042Substrate binding
Region256 – 2605Substrate binding
Motif259 – 2602GF motif that is essential for ArgX substrate specificity

Sites

Metal binding2371Magnesium 1
Metal binding2501Magnesium 1
Metal binding2501Magnesium 2
Metal binding2521Magnesium 2
Binding site871ATP
Binding site1271ATP
Binding site1921Substrate
Binding site2021ATP

Secondary structure

....................................................... 282
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q970U6 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: B1690D7F15D6E75F

FASTA28231,534
        10         20         30         40         50         60 
MRVVLIVDIV RQEEKLIAKA LEENKVQYDI INVAQEPLPF NKALGRYDVA IIRPVSMYRA 

        70         80         90        100        110        120 
LYSSAVLEAA GVHTINSSDV INVCGDKILT YSKLYREGIP IPDSIIALSA EAALKAYEQR 

       130        140        150        160        170        180 
GFPLIDKPPI GSWGRLVSLI RDVFEGKTII EHRELMGNSA LKAHIVQEYI QYKGRDIRCI 

       190        200        210        220        230        240 
AIGEELLGCY ARNIPPNEWR ANVALGGTPS NIEVDEKLKE TVVKAVSIVH GEFVSIDILE 

       250        260        270        280 
HPNKGYVVNE LNDVPEFKGF MVATNINVAQ KLVEYIKENY SK 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, Sulfolobus tokodaii strain7."
Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T. expand/collapse author list , Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.
DNA Res. 8:123-140(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
[2]"Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus."
Ouchi T., Tomita T., Horie A., Yoshida A., Takahashi K., Nishida H., Lassak K., Taka H., Mineki R., Fujimura T., Kosono S., Nishiyama C., Masui R., Kuramitsu S., Albers S.V., Kuzuyama T., Nishiyama M.
Nat. Chem. Biol. 9:277-283(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH LYSW; ADP AND MAGNESIUM, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH LYSW.
Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000023 Genomic DNA. Translation: BAB66577.1.
RefSeqNP_377468.1. NC_003106.2.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VPBX-ray1.80A/B/C/D1-282[»]
3VPCX-ray1.87A/B/C/D1-282[»]
ProteinModelPortalQ970U6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273063.ST1505.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB66577; BAB66577; STK_15050.
GeneID1459541.
KEGGsto:ST1505.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000228553.
KOK05827.
OMANIELEVY.

Enzyme and pathway databases

BioCycSTOK273063:GJC7-1677-MONOMER.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamPF08443. RimK. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameARGX_SULTO
AccessionPrimary (citable) accession number: Q970U6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: December 1, 2001
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references