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Q970U6

- ARGX_SULTO

UniProt

Q970U6 - ARGX_SULTO

Protein

Glutamate--LysW ligase ArgX

Gene

argE

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.By similarity

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871ATP
    Binding sitei127 – 1271ATP
    Binding sitei192 – 1921Substrate
    Binding sitei202 – 2021ATP
    Metal bindingi237 – 2371Magnesium 11 Publication
    Metal bindingi250 – 2501Magnesium 11 Publication
    Metal bindingi250 – 2501Magnesium 21 Publication
    Metal bindingi252 – 2521Magnesium 21 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi131 – 1377ATP
    Nucleotide bindingi167 – 17812ATPAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ligase activity Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-KW
    2. cellular protein modification process Source: InterPro
    3. lysine biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSTOK273063:GJC7-1677-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--LysW ligase ArgX (EC:6.3.2.-)
    Gene namesi
    Name:argE
    Synonyms:argX
    Ordered Locus Names:STK_15050
    OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
    Taxonomic identifieri273063 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001015: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 282282Glutamate--LysW ligase ArgXPRO_0000422990Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer By similarity. Homotetramer. Interacts with LysW.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi273063.ST1505.

    Structurei

    Secondary structure

    1
    282
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Helixi12 – 2312
    Beta strandi27 – 326
    Turni33 – 353
    Beta strandi38 – 414
    Helixi42 – 465
    Beta strandi48 – 525
    Helixi57 – 6913
    Beta strandi73 – 764
    Helixi78 – 847
    Helixi87 – 9610
    Beta strandi104 – 1074
    Helixi110 – 12011
    Beta strandi122 – 1276
    Turni133 – 1364
    Beta strandi138 – 1403
    Helixi143 – 15311
    Helixi159 – 1624
    Beta strandi164 – 1685
    Beta strandi172 – 18211
    Beta strandi185 – 1939
    Helixi203 – 2053
    Beta strandi208 – 2103
    Helixi216 – 22914
    Beta strandi232 – 24110
    Turni242 – 2443
    Beta strandi245 – 25410
    Helixi258 – 2647
    Helixi268 – 28013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VPBX-ray1.80A/B/C/D1-282[»]
    3VPCX-ray1.87A/B/C/D1-282[»]
    ProteinModelPortaliQ970U6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 277187ATP-graspPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni203 – 2042Substrate binding
    Regioni256 – 2605Substrate binding

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi259 – 2602GF motif that is essential for ArgX substrate specificity

    Sequence similaritiesi

    Belongs to the RimK family. LysX subfamily.Curated
    Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000228553.
    KOiK05827.
    OMAiNIELEVY.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR011870. LysX_arch.
    IPR016185. PreATP-grasp_dom.
    IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
    [Graphical view]
    PfamiPF08443. RimK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
    TIGR00768. rimK_fam. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q970U6-1 [UniParc]FASTAAdd to Basket

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    MRVVLIVDIV RQEEKLIAKA LEENKVQYDI INVAQEPLPF NKALGRYDVA    50
    IIRPVSMYRA LYSSAVLEAA GVHTINSSDV INVCGDKILT YSKLYREGIP 100
    IPDSIIALSA EAALKAYEQR GFPLIDKPPI GSWGRLVSLI RDVFEGKTII 150
    EHRELMGNSA LKAHIVQEYI QYKGRDIRCI AIGEELLGCY ARNIPPNEWR 200
    ANVALGGTPS NIEVDEKLKE TVVKAVSIVH GEFVSIDILE HPNKGYVVNE 250
    LNDVPEFKGF MVATNINVAQ KLVEYIKENY SK 282
    Length:282
    Mass (Da):31,534
    Last modified:December 1, 2001 - v1
    Checksum:iB1690D7F15D6E75F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAB66577.1.
    RefSeqiNP_377468.1. NC_003106.2.
    WP_010979555.1. NC_003106.2.

    Genome annotation databases

    EnsemblBacteriaiBAB66577; BAB66577; STK_15050.
    GeneIDi1459541.
    KEGGisto:ST1505.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000023 Genomic DNA. Translation: BAB66577.1 .
    RefSeqi NP_377468.1. NC_003106.2.
    WP_010979555.1. NC_003106.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VPB X-ray 1.80 A/B/C/D 1-282 [» ]
    3VPC X-ray 1.87 A/B/C/D 1-282 [» ]
    ProteinModelPortali Q970U6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273063.ST1505.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB66577 ; BAB66577 ; STK_15050 .
    GeneIDi 1459541.
    KEGGi sto:ST1505.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000228553.
    KOi K05827.
    OMAi NIELEVY.

    Enzyme and pathway databases

    BioCyci STOK273063:GJC7-1677-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProi IPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR011870. LysX_arch.
    IPR016185. PreATP-grasp_dom.
    IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
    [Graphical view ]
    Pfami PF08443. RimK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR02144. LysX_arch. 1 hit.
    TIGR00768. rimK_fam. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH LYSW; ADP AND MAGNESIUM, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH LYSW.
      Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

    Entry informationi

    Entry nameiARGX_SULTO
    AccessioniPrimary (citable) accession number: Q970U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2013
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3