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Q970U6

- ARGX_SULTO

UniProt

Q970U6 - ARGX_SULTO

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Protein

Glutamate--LysW ligase ArgX

Gene

argE

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent formation of a covalent bond between the amino group of glutamate and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.By similarity

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871ATP
Binding sitei127 – 1271ATP
Binding sitei192 – 1921Substrate
Binding sitei202 – 2021ATP
Metal bindingi237 – 2371Magnesium 11 Publication
Metal bindingi250 – 2501Magnesium 11 Publication
Metal bindingi250 – 2501Magnesium 21 Publication
Metal bindingi252 – 2521Magnesium 21 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi131 – 1377ATP
Nucleotide bindingi167 – 17812ATPAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-KW
  2. cellular protein modification process Source: InterPro
  3. lysine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1677-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--LysW ligase ArgX (EC:6.3.2.-)
Gene namesi
Name:argE
Synonyms:argX
Ordered Locus Names:STK_15050
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001015: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Glutamate--LysW ligase ArgXPRO_0000422990Add
BLAST

Interactioni

Subunit structurei

Homodimer (By similarity). Homotetramer. Interacts with LysW.By similarity1 Publication

Protein-protein interaction databases

STRINGi273063.ST1505.

Structurei

Secondary structure

1
282
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98Combined sources
Helixi12 – 2312Combined sources
Beta strandi27 – 326Combined sources
Turni33 – 353Combined sources
Beta strandi38 – 414Combined sources
Helixi42 – 465Combined sources
Beta strandi48 – 525Combined sources
Helixi57 – 6913Combined sources
Beta strandi73 – 764Combined sources
Helixi78 – 847Combined sources
Helixi87 – 9610Combined sources
Beta strandi104 – 1074Combined sources
Helixi110 – 12011Combined sources
Beta strandi122 – 1276Combined sources
Turni133 – 1364Combined sources
Beta strandi138 – 1403Combined sources
Helixi143 – 15311Combined sources
Helixi159 – 1624Combined sources
Beta strandi164 – 1685Combined sources
Beta strandi172 – 18211Combined sources
Beta strandi185 – 1939Combined sources
Helixi203 – 2053Combined sources
Beta strandi208 – 2103Combined sources
Helixi216 – 22914Combined sources
Beta strandi232 – 24110Combined sources
Turni242 – 2443Combined sources
Beta strandi245 – 25410Combined sources
Helixi258 – 2647Combined sources
Helixi268 – 28013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VPBX-ray1.80A/B/C/D1-282[»]
3VPCX-ray1.87A/B/C/D1-282[»]
ProteinModelPortaliQ970U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 277187ATP-graspPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 2042Substrate binding
Regioni256 – 2605Substrate binding

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi259 – 2602GF motif that is essential for ArgX substrate specificity

Sequence similaritiesi

Belongs to the RimK family. LysX subfamily.Curated
Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0189.
HOGENOMiHOG000228553.
KOiK05827.
OMAiNIELEVY.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view]
PfamiPF08443. RimK. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q970U6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVVLIVDIV RQEEKLIAKA LEENKVQYDI INVAQEPLPF NKALGRYDVA
60 70 80 90 100
IIRPVSMYRA LYSSAVLEAA GVHTINSSDV INVCGDKILT YSKLYREGIP
110 120 130 140 150
IPDSIIALSA EAALKAYEQR GFPLIDKPPI GSWGRLVSLI RDVFEGKTII
160 170 180 190 200
EHRELMGNSA LKAHIVQEYI QYKGRDIRCI AIGEELLGCY ARNIPPNEWR
210 220 230 240 250
ANVALGGTPS NIEVDEKLKE TVVKAVSIVH GEFVSIDILE HPNKGYVVNE
260 270 280
LNDVPEFKGF MVATNINVAQ KLVEYIKENY SK
Length:282
Mass (Da):31,534
Last modified:December 1, 2001 - v1
Checksum:iB1690D7F15D6E75F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66577.1.
RefSeqiNP_377468.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB66577; BAB66577; STK_15050.
GeneIDi1459541.
KEGGisto:ST1505.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66577.1 .
RefSeqi NP_377468.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VPB X-ray 1.80 A/B/C/D 1-282 [» ]
3VPC X-ray 1.87 A/B/C/D 1-282 [» ]
ProteinModelPortali Q970U6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273063.ST1505.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB66577 ; BAB66577 ; STK_15050 .
GeneIDi 1459541.
KEGGi sto:ST1505.

Phylogenomic databases

eggNOGi COG0189.
HOGENOMi HOG000228553.
KOi K05827.
OMAi NIELEVY.

Enzyme and pathway databases

BioCyci STOK273063:GJC7-1677-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProi IPR011761. ATP-grasp.
IPR013651. ATP-grasp_RimK-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR011870. LysX_arch.
IPR016185. PreATP-grasp_dom.
IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
[Graphical view ]
Pfami PF08443. RimK. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR02144. LysX_arch. 1 hit.
TIGR00768. rimK_fam. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH LYSW; ADP AND MAGNESIUM, SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH LYSW.
    Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7.

Entry informationi

Entry nameiARGX_SULTO
AccessioniPrimary (citable) accession number: Q970U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3