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Protein

Threonylcarbamoyl-AMP synthase

Gene

sua5

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine (By similarity). Probably catalyzes the conversion of L-threonine, HCO3-/CO2 and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Shows ATP hydrolysis activity in vitro, producing AMP.By similarity1 Publication

Catalytic activityi

L-threonine + ATP + HCO3- = L-threonylcarbamoyladenylate + diphosphate + H2O.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361L-threonine1 Publication
Binding sitei59 – 591ATP
Binding sitei63 – 631ATP
Binding sitei68 – 681L-threonine1 Publication
Binding sitei118 – 1181ATP
Binding sitei122 – 1221L-threonine1 Publication
Binding sitei142 – 1421L-threonine; via carbonyl oxygen1 Publication
Binding sitei144 – 1441ATP; via amide nitrogen
Binding sitei152 – 1521ATP
Binding sitei182 – 1821L-threonine1 Publication
Binding sitei196 – 1961ATP
Binding sitei237 – 2371ATP

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • double-stranded RNA binding Source: InterPro
  • L-threonylcarbamoyladenylate synthase Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1697-MONOMER.
BRENDAi2.7.7.87. 6166.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyl-AMP synthase (EC:2.7.7.87)
Short name:
TC-AMP synthase
Alternative name(s):
L-threonylcarbamoyladenylate synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Sua5
tRNA threonylcarbamoyladenosine biosynthesis protein Sua5
Gene namesi
Name:sua5
Ordered Locus Names:STK_15260
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 352351Threonylcarbamoyl-AMP synthasePRO_0000423850Add
BLAST

Interactioni

Subunit structurei

Monomer.3 Publications

Protein-protein interaction databases

STRINGi273063.ST1526.

Structurei

Secondary structure

1
352
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Beta strandi9 – 113Combined sources
Helixi14 – 2512Combined sources
Beta strandi30 – 323Combined sources
Beta strandi35 – 373Combined sources
Beta strandi39 – 435Combined sources
Helixi47 – 5711Combined sources
Beta strandi66 – 683Combined sources
Helixi72 – 787Combined sources
Beta strandi79 – 813Combined sources
Helixi84 – 9310Combined sources
Beta strandi95 – 10410Combined sources
Helixi110 – 1134Combined sources
Beta strandi117 – 1226Combined sources
Helixi127 – 13610Combined sources
Beta strandi140 – 1423Combined sources
Beta strandi144 – 1463Combined sources
Helixi156 – 1638Combined sources
Turni164 – 1663Combined sources
Beta strandi167 – 1726Combined sources
Beta strandi183 – 1864Combined sources
Beta strandi188 – 1914Combined sources
Beta strandi193 – 1964Combined sources
Helixi202 – 2087Combined sources
Beta strandi240 – 2467Combined sources
Helixi249 – 2513Combined sources
Helixi252 – 2598Combined sources
Turni260 – 2623Combined sources
Beta strandi265 – 2706Combined sources
Helixi271 – 2733Combined sources
Helixi275 – 2773Combined sources
Beta strandi280 – 2867Combined sources
Helixi291 – 30717Combined sources
Beta strandi311 – 3166Combined sources
Turni321 – 3233Combined sources
Helixi324 – 33411Combined sources
Beta strandi339 – 3424Combined sources
Helixi343 – 3497Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EQAX-ray1.80A1-352[»]
3AJEX-ray1.80A1-352[»]
4E1BX-ray1.80A1-352[»]
ProteinModelPortaliQ970S6.
SMRiQ970S6. Positions 2-351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ970S6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 200187YrdC-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SUA5 family.Curated
Contains 1 YrdC-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01952. Archaea.
COG0009. LUCA.
HOGENOMiHOG000076160.
KOiK07566.
OMAiAIRMPDH.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR005145. SUA5.
IPR010923. t(6)A37_SUA5.
IPR006070. YrdC-like_dom.
[Graphical view]
PfamiPF03481. SUA5. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
[Graphical view]
PIRSFiPIRSF004930. Tln_factor_SUA5. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00057. TIGR00057. 1 hit.
PROSITEiPS51163. YRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q970S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQIIKIDPL NPEIDKIKIA ADVIRNGGTV AFPTETVYGL GANAFDGNAC
60 70 80 90 100
LKIFQAKNRP VDNPLIVHIA DFNQLFEVAK DIPDKVLEIA QIVWPGPLTF
110 120 130 140 150
VLKKTERVPK EVTAGLDTVA VRMPAHPIAL QLIRESGVPI AAPSANLATR
160 170 180 190 200
PSPTKAEDVI VDLNGRVDVI IDGGHTFFGV ESTIINVTVE PPVLLRPGPF
210 220 230 240 250
TIEELKKLFG EIVIPEFAQG KKEAEIALAP GMKYKHYAPN TRLLLVENRN
260 270 280 290 300
IFKDVVSLLS KKYKVALLIP KELSKEFEGL QQIILGSDEN LYEVARNLFD
310 320 330 340 350
SFRELDKLNV DLGIMIGFPE RGIGFAIMNR ARKASGFSII KAISDVYKYV

NI
Length:352
Mass (Da):38,802
Last modified:December 1, 2001 - v1
Checksum:iA1D5B9FA171BD294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66597.1.
RefSeqiWP_010979575.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB66597; BAB66597; STK_15260.
GeneIDi1459561.
KEGGisto:STK_15260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66597.1.
RefSeqiWP_010979575.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EQAX-ray1.80A1-352[»]
3AJEX-ray1.80A1-352[»]
4E1BX-ray1.80A1-352[»]
ProteinModelPortaliQ970S6.
SMRiQ970S6. Positions 2-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST1526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB66597; BAB66597; STK_15260.
GeneIDi1459561.
KEGGisto:STK_15260.

Phylogenomic databases

eggNOGiarCOG01952. Archaea.
COG0009. LUCA.
HOGENOMiHOG000076160.
KOiK07566.
OMAiAIRMPDH.

Enzyme and pathway databases

BioCyciSTOK273063:GJC7-1697-MONOMER.
BRENDAi2.7.7.87. 6166.

Miscellaneous databases

EvolutionaryTraceiQ970S6.
PROiQ970S6.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR005145. SUA5.
IPR010923. t(6)A37_SUA5.
IPR006070. YrdC-like_dom.
[Graphical view]
PfamiPF03481. SUA5. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
[Graphical view]
PIRSFiPIRSF004930. Tln_factor_SUA5. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00057. TIGR00057. 1 hit.
PROSITEiPS51163. YRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUA5_SULTO
AccessioniPrimary (citable) accession number: Q970S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.