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Protein

Threonylcarbamoyl-AMP synthase

Gene

sua5

Organism
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine (By similarity). Probably catalyzes the conversion of L-threonine, HCO3-/CO2 and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Shows ATP hydrolysis activity in vitro, producing AMP.By similarity1 Publication

Catalytic activityi

L-threonine + ATP + HCO3- = L-threonylcarbamoyladenylate + diphosphate + H2O.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36L-threonine1 Publication1
Binding sitei59ATP1
Binding sitei63ATP1
Binding sitei68L-threonine1 Publication1
Binding sitei118ATP1
Binding sitei122L-threonine1 Publication1
Binding sitei142L-threonine; via carbonyl oxygen1 Publication1
Binding sitei144ATP; via amide nitrogen1
Binding sitei152ATP1
Binding sitei182L-threonine1 Publication1
Binding sitei196ATP1
Binding sitei237ATP1

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • double-stranded RNA binding Source: InterPro
  • L-threonylcarbamoyladenylate synthase Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.87. 6166.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonylcarbamoyl-AMP synthase (EC:2.7.7.87)
Short name:
TC-AMP synthase
Alternative name(s):
L-threonylcarbamoyladenylate synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Sua5
tRNA threonylcarbamoyladenosine biosynthesis protein Sua5
Gene namesi
Name:sua5
Ordered Locus Names:STK_15260
OrganismiSulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Taxonomic identifieri273063 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001015 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004238502 – 352Threonylcarbamoyl-AMP synthaseAdd BLAST351

Interactioni

Subunit structurei

Monomer.3 Publications

Protein-protein interaction databases

STRINGi273063.ST1526.

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi9 – 11Combined sources3
Helixi14 – 25Combined sources12
Beta strandi30 – 32Combined sources3
Beta strandi35 – 37Combined sources3
Beta strandi39 – 43Combined sources5
Helixi47 – 57Combined sources11
Beta strandi66 – 68Combined sources3
Helixi72 – 78Combined sources7
Beta strandi79 – 81Combined sources3
Helixi84 – 93Combined sources10
Beta strandi95 – 104Combined sources10
Helixi110 – 113Combined sources4
Beta strandi117 – 122Combined sources6
Helixi127 – 136Combined sources10
Beta strandi140 – 142Combined sources3
Beta strandi144 – 146Combined sources3
Helixi156 – 163Combined sources8
Turni164 – 166Combined sources3
Beta strandi167 – 172Combined sources6
Beta strandi183 – 186Combined sources4
Beta strandi188 – 191Combined sources4
Beta strandi193 – 196Combined sources4
Helixi202 – 208Combined sources7
Beta strandi240 – 246Combined sources7
Helixi249 – 251Combined sources3
Helixi252 – 259Combined sources8
Turni260 – 262Combined sources3
Beta strandi265 – 270Combined sources6
Helixi271 – 273Combined sources3
Helixi275 – 277Combined sources3
Beta strandi280 – 286Combined sources7
Helixi291 – 307Combined sources17
Beta strandi311 – 316Combined sources6
Turni321 – 323Combined sources3
Helixi324 – 334Combined sources11
Beta strandi339 – 342Combined sources4
Helixi343 – 349Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EQAX-ray1.80A1-352[»]
3AJEX-ray1.80A1-352[»]
4E1BX-ray1.80A1-352[»]
ProteinModelPortaliQ970S6.
SMRiQ970S6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ970S6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 200YrdC-likePROSITE-ProRule annotationAdd BLAST187

Sequence similaritiesi

Belongs to the SUA5 family.Curated
Contains 1 YrdC-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01952. Archaea.
COG0009. LUCA.
HOGENOMiHOG000076160.
KOiK07566.
OMAiAIRMPDH.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR005145. SUA5.
IPR010923. t(6)A37_SUA5.
IPR006070. YrdC-like_dom.
[Graphical view]
PfamiPF03481. SUA5. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
[Graphical view]
PIRSFiPIRSF004930. Tln_factor_SUA5. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00057. TIGR00057. 1 hit.
PROSITEiPS51163. YRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q970S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQIIKIDPL NPEIDKIKIA ADVIRNGGTV AFPTETVYGL GANAFDGNAC
60 70 80 90 100
LKIFQAKNRP VDNPLIVHIA DFNQLFEVAK DIPDKVLEIA QIVWPGPLTF
110 120 130 140 150
VLKKTERVPK EVTAGLDTVA VRMPAHPIAL QLIRESGVPI AAPSANLATR
160 170 180 190 200
PSPTKAEDVI VDLNGRVDVI IDGGHTFFGV ESTIINVTVE PPVLLRPGPF
210 220 230 240 250
TIEELKKLFG EIVIPEFAQG KKEAEIALAP GMKYKHYAPN TRLLLVENRN
260 270 280 290 300
IFKDVVSLLS KKYKVALLIP KELSKEFEGL QQIILGSDEN LYEVARNLFD
310 320 330 340 350
SFRELDKLNV DLGIMIGFPE RGIGFAIMNR ARKASGFSII KAISDVYKYV

NI
Length:352
Mass (Da):38,802
Last modified:December 1, 2001 - v1
Checksum:iA1D5B9FA171BD294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66597.1.
RefSeqiWP_010979575.1. NC_003106.2.

Genome annotation databases

EnsemblBacteriaiBAB66597; BAB66597; STK_15260.
GeneIDi1459561.
KEGGisto:STK_15260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000023 Genomic DNA. Translation: BAB66597.1.
RefSeqiWP_010979575.1. NC_003106.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EQAX-ray1.80A1-352[»]
3AJEX-ray1.80A1-352[»]
4E1BX-ray1.80A1-352[»]
ProteinModelPortaliQ970S6.
SMRiQ970S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273063.ST1526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB66597; BAB66597; STK_15260.
GeneIDi1459561.
KEGGisto:STK_15260.

Phylogenomic databases

eggNOGiarCOG01952. Archaea.
COG0009. LUCA.
HOGENOMiHOG000076160.
KOiK07566.
OMAiAIRMPDH.

Enzyme and pathway databases

BRENDAi2.7.7.87. 6166.

Miscellaneous databases

EvolutionaryTraceiQ970S6.
PROiQ970S6.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR005145. SUA5.
IPR010923. t(6)A37_SUA5.
IPR006070. YrdC-like_dom.
[Graphical view]
PfamiPF03481. SUA5. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
[Graphical view]
PIRSFiPIRSF004930. Tln_factor_SUA5. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00057. TIGR00057. 1 hit.
PROSITEiPS51163. YRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUA5_SULTO
AccessioniPrimary (citable) accession number: Q970S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.